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Hello,I failed to identify this anamorph, which gr

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Hi again Could you help me with this paper? NANN

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anamorph on Pistacia-leaves in Portugal
Lothar Krieglsteiner, 14-05-2019 18:24
Lothar Krieglsteiner

9.4.19, near Aljezur (west Coast).


The fungus was very common there and I also found it elsewhere.


The conidia were about 3,5 µm in diameter.


Who knows such a fungus?


Best regards, Lothar

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David Malloch, 14-05-2019 18:49
David Malloch
Re : anamorph on Pistacia-leaves in Portugal
Hello Lothar,

I cannot be certain from your photographs, but I think your fungus on Pistacia might be Dichlaena lentisci Durieu & Mont. It was originally descrtibed from Algeria and is only rarely reported. According to Malloch & Cain (https://www.nrcresearchpress.com/doi/abs/10.1139/b72-335) it has an Aspergillus anamorph. If you find more and can send it to me I believe I can get it into culture and arranged to have it sequenced.

Check out the illustration on its MycoBank page. It must be from the original 1849 paper.

David
Lothar Krieglsteiner, 14-05-2019 19:02
Lothar Krieglsteiner
Re : anamorph on Pistacia-leaves in Portugal

Hello David


thank you very much for your answer and your suggestion. Yes, Dichlaena lentisci looks very similar - as far as I see in the small illustration on mycobank.


Unfortunately, I have no access to the paper you showed me with your other link (your paper).


I am back home since some weeks, and I only can send dried material. If you are interested in this, pleas tell me. I will go to the Algarve again, in future, and then I will try to find more.


Best regards and thanks, again,


Lothar

Martin Bemmann, 14-05-2019 19:13
Martin Bemmann
Re : anamorph on Pistacia-leaves in Portugal
Paper sent.

Grüße

Martin
Lothar Krieglsteiner, 14-05-2019 19:20
Lothar Krieglsteiner
Re : anamorph on Pistacia-leaves in Portugal

Hello Martin,


thank you very much for the paper - I received it well.


@David:


I will re-examine my material in the next days and will write again.


Thanks again, and best regards,


Lothar

David Malloch, 14-05-2019 19:30
David Malloch
Re : anamorph on Pistacia-leaves in Portugal
Hello Lothar,

It would probably be possible to obtain a culture from the dried material. I think it is quite resistant to drying.

I have four pictures of this fungus taken long ago that I put on a web page at http://website.nbm-mnb.ca/mycologywebpages/NaturalHistoryOfFungi/Eurotiales.html. The pictures are not very good, but they might help confirm your collection.

David
Lothar Krieglsteiner, 16-05-2019 14:22
Lothar Krieglsteiner
Re : anamorph on Pistacia-leaves in Portugal

Hello David and other readers,


today I re-examined the specimen.


First I mounted with Cotton Blue. No circular band seen, nothing else more.


Then I mounted in Carbol Fuchsine. No circular band. In my second attempt I found asci - this was great. So - the fungus should be related to Dichlaena lentisci.


But - although I have a lot of fantasy - I was not able to see a pale band on the spores.


What do you think?


If I should send you a specimen - to what address?


Best regards, Lothar

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David Malloch, 16-05-2019 16:27
David Malloch
Re : anamorph on Pistacia-leaves in Portugal
Hello Lothar,

Good photos! Now we see asci. Promising.

If you can send me some material I will attempt to grow it in culture. If the ascomata are not too old the ascospores should still be viable. Germination will reveal if there is a germ slit. If there is one, the spores should open like a clam when they germinate.

My address is:

Dr. David Malloch

New Brunswick Museum

277 Douglas Avenue

Saint John, New Brunswick, Canada

E2K 1E5

Regards,

David