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17-02-2016 22:49

Ueli Graf Ueli Graf

Hello, I'm looking for  Donadini J.C.  1981 Le g

14-08-2022 18:08

Edvin Johannesen Edvin Johannesen

Found on deciduous wood (probably Betula) in subal

14-08-2022 15:50

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello,this Orbilia grew on a strongly rotten branc

13-08-2022 17:18

Stephen Mifsud Stephen Mifsud

I assumed these are weak colonies of Neurospora on

10-08-2022 15:45

Pierre-Yves Julien

Récolte le 31/07/2022 – Ozoir-la-Ferrière (77)

04-07-2022 18:45

Enrique Rubio Enrique Rubio

I can find no suitable answer for this white Bryos

11-08-2022 00:45

Stefan Jakobsson

On a thin deciduous stick (Salix/Alnus/Betula) on

10-08-2022 18:45

François Bartholomeeusen

Good evening all, On 5 August 2022, a friend gave

11-08-2022 08:16

Josep Torres Josep Torres

Hola.Un muy diminuto Pirenomyceto sobre un tronco

10-08-2022 09:11

Josep Torres Josep Torres

Hola.Un Piremomyceto recogido este pasado domingo

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Chris
Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.