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01-06-2025 09:37

Charles Aron Charles Aron

Hi All, I found this Octospora growing with liver

06-07-2025 19:36

Castillo Joseba Castillo Joseba

me mandan el material de Galicia (España) recolec

05-07-2025 12:38

Åge Oterhals

I found this pyrenomycetous fungi in pine forest o

04-07-2025 20:12

Josep Torres Josep Torres

Hello.A fungus growing on the surface of a trunk o

20-06-2025 08:33

Josep Torres Josep Torres

Hello.Small, blackish, mucronated surface grains s

28-06-2025 16:00

Josep Torres Josep Torres

Hello.A tiny fungus shaped like globose black grai

04-07-2025 12:43

Castillo Joseba Castillo Joseba

me mandan el material seco de Galicia (España) 

03-07-2025 18:40

Castillo Joseba Castillo Joseba

me mandas el material seco de Galicia (España) re

03-07-2025 20:08

Francois Guay Francois Guay

I found this interesting yellowish asco growing on

01-07-2025 23:37

Josep Torres Josep Torres

Hello.A Pleosporal symbiotic organism located and

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Chris
Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.