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30-04-2025 01:29

Dactylellina candida/candidum?
David Chapados David Chapados

Hi, I found Dactylellina candida/candidum, recent

29-04-2025 09:13

Est-ce que Mollisia faginea existe?
Louis DENY

Bonjour forumVosges du sud, ballon d'Alsace altitu

28-04-2025 12:51

Pyrenopeziza atrata on Angelica silvestris
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Substrate: Angelica sylvesrisSpore mass: 8.4 - 11.

12-05-2013 13:31

Lophodermium on Picea abies
Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Dear mycologists,could someone give me an advice a

18-04-2025 23:16

Mollisia
Robin Pétermann Robin Pétermann

Bonjour, Voici une probable Mollisia, genre que j

26-04-2025 07:15

Paranectria?
Dragiša Savic

Hello everyone,the lichen is Candelariella aurella

27-04-2025 15:54

literature search
Martin Bemmann Martin Bemmann

Can somebody provide this article from a Leningrad

14-04-2025 15:11

Need help with 6 coprophilous species
Lennert Gees

Greetings!For my master's dissertation I work on c

26-04-2025 16:01

Michel Delpont
Jacques Fournier Jacques Fournier

J'ai le regret d'informer les membres du forum qui

26-04-2025 19:32

Arachnopeziza sp.
Francois Guay Francois Guay

I found these small Arachnopeziza sp. growing on d

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Chris
Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.