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Mot de passe perdu? S'inscrire

18-07-2025 23:03

Help with some conidia.
Josep Torres Josep Torres

Hello.Fruitings between 51 and 130 microns in tota

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Una para ID 833
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De ayer en bosque de hayas y abetos, en tieraEjemp

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Hello,I have trouble distinguishing above mention

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Bonjour, Voici une espèce de  (?) Hyaloscyphace

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literature request
Angel Pintos Angel Pintos

Hello, does anyone have access to the following ar

16-01-2023 21:31

Troposporella fumosum => Troposporella monilipes
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Hello, Nearby the find of Calycina claroflava on

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Yanick BOULANGER

BonjourAutre dossier laissé en suspendJe viens de

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BonjourJ'ai un dossier Jackrogersella qui est rest

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Olla aff. millepunctata (croziers +) on Rubus idaeus
Gernot Friebes

Hi,I wanted to share this collection on Rubus idae

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Rutstroemia ulmariae(?) on Saxifraga rotundifolia
Gernot Friebes

Hi,do you think this collection could be R. ulmari

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Chris
Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.