11-04-2026 15:45
Zuzana Sochorová (Egertová)
Please, could anyone send me this paper?Moyne G.,
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Artem PtukhaHello, I am seeking assistance with the identific
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Michel Hairaud
Chers amis d'Ascofrance , voici une très bonne no
11-04-2026 10:10
Michel Hairaud
Dear Ascofrance members, here is some very good ne
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Gernot FriebesHi,ascospores are 1- to 3-septate, approximately
10-04-2026 15:51
William Slosse
Hello everyone, On 08/04/26, I found a growth sit
09-04-2026 15:25
Jac GelderblomOn bare soil between mosses Ifound an asco I deter
09-04-2026 13:55
Thomas Læssøehttps://svampe.databasen.org/observations/10589176
09-04-2026 10:12
Thomas Læssøehttps://svampe.databasen.org/observations/10587061
Calycellina spiraeae spores
Chris Yeates,
21-10-2021 13:26
Bonjour tous
While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".
I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?
Cordialement, Chris
Hans-Otto Baral,
21-10-2021 17:13
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)
Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio,
21-10-2021 17:37
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates,
21-10-2021 20:23
Re : Calycellina spiraeae spores
Thank you both for these comments.
Chris
Hans-Otto Baral,
21-10-2021 20:51
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio,
21-10-2021 20:59
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral,
21-10-2021 21:55
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio,
24-10-2021 09:30
Re : Calycellina spiraeae spores
Thanks, Zotto.