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09-06-2025 16:18

Sylvie Le Goff

BonjourPourrais je avoir votre avis sur cet ascome

09-06-2025 12:50

Joaquin Martin

Hi,Last week I found this Hymenoscyphus on Rubus.S

09-06-2025 22:28

Edmond POINTE Edmond POINTE

Bonjour,Apothecie pulvinée, blanche 0.4 x 1mm. As

09-06-2025 10:32

Elisabeth Stöckli

Bonjour,Trouvé sur une branche morte et décortiq

09-06-2025 16:36

David Malloch David Malloch

As far as I know, this species has yet to be assig

08-06-2025 18:03

éric ROMERO éric ROMERO

Bonjour à tous, Une récolte alpestre discrète

07-06-2025 15:39

Edvin Johannesen Edvin Johannesen

A friend sent me a few Betula seeds with tiny blac

08-06-2025 14:55

Edvin Johannesen Edvin Johannesen

Ascomata only ca. 1 mm, erumpent on very thin Sali

06-06-2025 12:12

Hans-Otto Baral Hans-Otto Baral

Dear all I want to ask you if you have any recent

04-06-2025 15:10

Stefan Jakobsson

Hi forum, On a herbaceous stem, possibly Aegopodi

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Aff. Heterosphaeria on Populus tremula wood
Edvin Johannesen, 13-01-2021 19:14
Edvin JohannesenI though I'd just ask you about this tiny discomycete, which I struggle with. It is only about 0.2-0.4 mm in diameter. It was growing on a Populus tremula log, directly on the wood. The wood is hardly decomposed and very firm. It was collected last May, but I only recently received the dried specimen. It seems like the hymenium in many of the apothecia are now eaten by insects or have decomposed. Thus quite difficult to make nice, thin sections, so I apologise for that.

I am not even sure what family we're in. Initially, when I saw it in wet condition, I thought it was erumpent and was thinking Coccomyces or along those lines. Indeed some of the younger ascomata appear to be erumpent, but in the dried material I see that the dark-rimmed, somewhat dentate apothecia are sitting directly on the wood, anchored by a fringe of white hyphae (anchoring hyphae/subiculum). The hymenium is, when fresh whitish, when dry more greyish and somewhat pruinose.


The spores measure 12-15 x 5 microns, mostly 3-septate, hyaline, smooth. Some spores are smaller (7 x 4) and 1-septate. Many spores are germinating from one or both ends – I guess the specimen is over-mature. What I think are paraphyses are orange-tipped (irregularly flame-like). There are dark, transversely roughened hyphae in the excipulum (fringe) and some spherical cells in what may be subhymenium. The ascus tips are IKI+ (blue in Baral'sche Lösung with no KOH pre-treatment). Structures in the excipulum are stained greenish-blue with IKI, too, but I am not sure that it's due to amyloidity.


Here's a link to some photos. Photo names indicate the medium (KOH, IKI, PC (phase contrast)).


https://www.dropbox.com/sh/4prdmaszwi682ys/AABEAjm33Pf5d4b0lAUx0cnLa?dl=0


The best fit is in the genus Heterosphaeria, but I'm not sure if any species in that genus grows on wood.  Are we in Phacidiaceae?


Suggestions appreciated.  Thanks!  (I hope the link works)

Hans-Otto Baral, 13-01-2021 21:43
Hans-Otto Baral
Re : Aff. Heterosphaeria on Populus tremula wood
Too overmature to say much. Durella (Xylogramma) atrocyanea would be inamyloid. Any idea of the paraphyses?
Edvin Johannesen, 13-01-2021 22:13
Edvin Johannesen
Re : Aff. Heterosphaeria on Populus tremula wood
Difficult to find an intact hymenium. I thought maybe the three phase contrast images showed paraphyses with the golden structures apically. I will try again to see if I can find intact hymenium with paraphyses, but several attempts have failed so far.