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Castillo Joseba
me mandan el material seco de Galicia (España)
Phaeohelotium fagineum ?
Thierry Blondelle,
04-06-2024 21:26
Bonjour,Je vous soumets cette récolte sur faîne (cupules of Fagus) que j'ai déterminée comme étant Paeohelotium fagineum d'après la clé de Declercq (est-ce la dernier version pour les Hymenoscyphus ?)
J'ai tout de même quelques interrogations sur mon interprétation de la micro:
- Je distingue 3 couches succesives sous-hyméniales (une couche d'excipulum hymenial de type intricata, une couche intermédiaire de type porrecta, une couche d'excipulum ectal basale de type globulosa-angularis.
- Un OCI voisin de 2 plutôt que de 0-1
- des spores à 1-2 cellules. Est-ce un critère récurrent ?
Mes observations sont-elles suffisantes et confirment-elles l'espèce ?
Merci pour vos avis.
Salutations
Thierry
Hans-Otto Baral,
04-06-2024 23:14
Re : Phaeohelotium fagineum ?
P. fagineum is a species with a short stout stipe. The living paraphyses do not contain VBs in your case which excludes a lot of Hymenoscyphi. Important would be to clarfy whether the spores are ejected when non-septate or when septate. If you find living mature asci you will see that. Also iodine reaction and croziers are required.
Thierry Blondelle,
05-06-2024 07:28
Re : Phaeohelotium fagineum ?
Thanks Zotto for these new ways of research.
Thierry Blondelle,
05-06-2024 23:12
Re : Phaeohelotium fagineum ?
Here are new élements of observation
- Spores are two-cells septated in both living asci and after expulsion.
- IKI+on asci
- For the croziers, i'm not sure. To confirm on the picture.
Can we always consider Hymenoscyphi ?
- Spores are two-cells septated in both living asci and after expulsion.
- IKI+on asci
- For the croziers, i'm not sure. To confirm on the picture.
Can we always consider Hymenoscyphi ?
Hans-Otto Baral,
06-06-2024 08:50
Re : Phaeohelotium fagineum ?
The apical ring looks Hymenoscyphus-like, but that type occurs also elsewhere. I see no living asci, we cannot be sure about the expulsion. And for croziers I am similarly uncertain, one needs the cel below, unruptured from the ascus.
My first idea was H. varicosporoides, but I remembered that species only with a short stalk. Now when looking in my folder there are several with long stalks (e.g. 18.II.2015, J. Balda). Also the original description refers to a long-stalked fungus. So I think this is a good option. The fungus has an interesting anamorph adapted to running or standing water.
Thierry Blondelle,
06-06-2024 13:52
Re : Phaeohelotium fagineum ?
Thanks a lot. The fungus rised on beechnuts fallen to the edge of a little stream. This confirm it is a species of damp places.
I am going to try another micros. How to identify living asci ?
I am going to try another micros. How to identify living asci ?
Hans-Otto Baral,
06-06-2024 17:17
Re : Phaeohelotium fagineum ?
Well, sorry, I wrote this 100x in this forum. Please go to my old homepage, the front page illustrates without words.