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Pilobolus kleinii and P. crystallinus
Riet van Oosten, 06-09-2020 17:03
Riet van Oosten
Hello,

On 02-09-2020 I took home some cow dung (Black Angus cows)

Today the Pilobolus appeared :-)

Is this Pilobolus kleinii?

I used the key:

Sporangia not umbonate - Sporangiophore under 5 mm, trophocyst within the substratum - Spores ellipsoid - Spores 7.5-19.5 - Spores pale yellow = P. crystallinus - Spores deeper yellow/orange = P. kleinii

Are the spores yellow enough for P. kleinii?

Spore sizes: Me=10.2 x 6.4 µm,  N=10

Greetings, Riet
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Hans de Jong, 06-09-2020 22:12
Hans de Jong
Re : Pilobolus kleinii
Hallo Riet,Wat gaaf dat je opnieuw Pilobolus hebt gevonden. Toevallig hebben Mark en ik gisteren ook P. kleinii gevonden. Ik vind het verschil tussen 'pale yellow' en 'yellow/orange' moeilijk. Op basis van de sporenmaat zou ik eerder kiezen voor P. crystallinus? Ben heel benieuwd wat Malcolm Greaves (de maker van de sleutel) hiervan vindt. Hieronder de foto van de sporen die Malcolm Greaves voor mij heeft bevestigd. 
Groet Hans
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Malcolm Greaves, 07-09-2020 01:15
Malcolm  Greaves
Re : Pilobolus kleinii
Hello Riet
You quote the spores as 7.5 -19.5 but looking at your micrograph they all look to be in the 7.5-9.5 range. Which is right? The colour does tend to vary but your spores do look quite pale for klenii so I think P crystalinus is the most likely. (unless the spores do reach 19.5)
Mal
Riet van Oosten, 07-09-2020 08:13
Riet van Oosten
Re : Pilobolus kleinii
Hello Malcolm,
"Spores 7.5 - 19.5" is a quotation from the key.
The spore sizes I measured:  Me: 10.2 x 6.4 µm, N = 10
So I will name this find P. cristallinus!
Thank you very much for your help!
Riet


To be sure ...
This afternoon I will measure the spores ejected to the lid of the plastic box.


To be continued Hans :-)  Dank je voor jouw reactie!

Malcolm Greaves, 07-09-2020 09:54
Malcolm  Greaves
Re : Pilobolus kleini? No = P. cristallinus
Keep watching the sample I recently had three species develop over a short period.
Riet van Oosten, 07-09-2020 17:30
Riet van Oosten
Re : Pilobolus kleinii and P. crystallinus
Thank you so much for your advice Malcolm!
The sporangia on the lid showed already two kinds of spores.
So nice (and very educational) to see the difference!

The larger and deeper yellow/orange spores are: Me 15.2 x 9.6 µm N=10 (= P. kleinii)

Key:
Spores 7.5-11 x 5-7.5 µm, pale yellow ...................................................P. crystallinus
Spores 10-19.5 x 5.5-9.5 µm, usually deeper yellow/orange....................P. kleinii




It was very hard to find a fruitbody of P. kleinii, but in the end I succeeded :-)

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Hans de Jong, 08-09-2020 10:31
Hans de Jong
Re : Pilobolus kleinii and P. crystallinus
Kijk! De aanhouder wint. 
Mooi succes. Groet
Hans
Riet van Oosten, 08-09-2020 12:36
Riet van Oosten
Re : Pilobolus kleinii and P. crystallinus
Dank je wel Hans! Zo leuk deze "ontdekkingen"!
Groetjes, Riet
Joop van der Lee, 17-09-2020 08:34
Joop van der Lee
Re : Pilobolus kleinii and P. crystallinus
Hallo Riet,
I do have the following documentation if you are interested:

Sul genere Pilobolus Tode 1874. Contributo allo studio dei funghi fimicoli XXIX.

The biology and taxonomy of Pilobolus. Researches on fungi volume VI.

Species of Pilobolus and Pilaira from India.

Taxa of Pilaira (Mucorales, Zygomycota) from China


Joop

Riet van Oosten, 17-09-2020 12:17
Riet van Oosten
Re : Pilobolus kleinii and P. crystallinus
Very nice to receive Joop!
Greetings, Riet