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19-08-2025 20:58

Ethan Crenson

Hi all, Here is what I believe to be a Hymenoscyp

12-08-2025 19:44

Enrique Rubio Enrique Rubio

Could someone send me a pdf copy of this article?S

18-08-2025 23:15

Zoe Vélez Zoe Vélez

Hola foro, gracias por aceptar la creación de mi

18-08-2025 15:17

Lothar Krieglsteiner Lothar Krieglsteiner

... on 6.7.25 in a subarctic mire near a small lak

18-08-2025 15:07

Lothar Krieglsteiner Lothar Krieglsteiner

.. 20.7.25, in subarctic habital. The liverwort i

19-08-2025 16:27

Paul Cannon

Hello all I have spent some time trying to work o

18-08-2025 22:59

Yanick BOULANGER

BonsoirVoici un asco récolté le 08/08/2025Comme

18-08-2025 16:01

Lothar Krieglsteiner Lothar Krieglsteiner

.. on water-soaked Betula wood lying in a small st

18-08-2025 15:35

Lothar Krieglsteiner Lothar Krieglsteiner

.. in subarctic forest at side of small stream, ac

18-08-2025 17:52

Yanick BOULANGER

BonjourAu fil des conversations, j'ai lu que Hyalo

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Aquatic ascomycete.
Josep Torres, 13-06-2025 09:41
Josep TorresHello.
A cerebriform ascomycete sprouting scattered on the surface of a trunk submerged in a clear stream.
Ascomata measuring up to 4 mm long and 2 to 2.5 mm wide, I was particularly struck by how tough and tenacious these ascomata were.
Very thin conidiophores arranged parallel in a palisade-like pattern produce globose conidia with a large lipid droplet inside that occupies three-quarters of the spore.
An amyloid reaction is observed from the base to the middle of the hymenium with Melzer's Reagent.
No apparent reaction to KOH.
Some of the images show clusters of these supposed conidia, which I wouldn't completely rule out asci.
These presumed conidia measured in water:
(3.6) 3.8 - 4.4 (4.7) × (2.9) 3.2 - 3.8 (4) µm
Q = 1 - 1.3 (1.4) ; N = 40
Me = 4.1 × 3.6 µm ; Qe = 1.1
I admit I don't even know where the air is coming from.
Any opinions would be welcome.
Thank you very much in advance.
Best regards.
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Josep Torres, 17-06-2025 14:09
Josep Torres
Re : Aquatic ascomycete.
Any suggestions.......
Josep Torres, 20-07-2025 14:34
Josep Torres
Re : Aquatic ascomycete.
Hello.
In case anyone is interested, the results of the molecular analysis of the ITS gene have determined that it is an anamorph of Ascocoryne sp. Based on the current data available in Genbank, we cannot be more precise.
I'm adding the phylogenetic tree.
Best regards.
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Francois Guay, 22-07-2025 18:39
Francois Guay
Re : Aquatic ascomycete.
Very interesting, thank you for the follow-up!
Hans-Otto Baral, 26-07-2025 09:29
Hans-Otto Baral
Re : Aquatic ascomycete.
Hi Josep
by accident I looked again in your thread. What a shame that I did not realize this being an Ascocoryne! Now the things are clear thanks to your DNA analysis.
I annotated your tree below but could not name all clades because GenBank is presently out of order.
As your sample is identical with OP023244, I conclude that it is also identical with a Polish sample on Salix not in GenBank (BRA CR32406), which had both conidiomata and apothecia, the latter regrettably overmature. It is so far the only case with apothecia within this "laurisilvae-clade".
A. laurisilvae is only known from its anamorph. It looks very much like yours, despite the genetic distance (see below).
Do you have any idea about the substrate? It looks like a deciduous tree. Do you have the wood to check its anatomy?
I would be pleased to receive the collection data, also the sequence and its ab1 file. And it would be great if we could use your photos in our work. Do you have a scale for your pics?

Very strange is the amyloid reaction, we did not see that in any anamorph of Ascocoryne!

Zotto
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Josep Torres, 26-07-2025 17:18
Josep Torres
Re : Aquatic ascomycete.
Hello Zotto.
Thanks for your prompt response.
You already have everything in your email.
Best regards.