J'ai récolté dernièrement des Scutellinia à spores rondes que je pense être S. minor ou S. barlae. Les spores subglobuleuses et l'ornementation quelque peu hétérogène me font pencher pour S. minor. Qu'en pensez-vous ?

L'aspect général est typique du genre Scutellinia. Brièvement, apothécie < 1 cm, d'un bel orange uniforme, à marge un peu plus claire. Surface externe couverte de poils courts.

Spores subglobuleuses (Q = 1.1), (15) 17 +/- 0.87 (19) x (14) 15 +/- 1.1 (18) µm [(min) moyenne +/- écart-type (max), N = 14, mesures effectuées dans le bleu coton lactique, ornementation exclue], à verrues arrondies grossièrement homogènes (quelques-unes plus grosses) de (1.2) 1.5 +/- 0.1 (1.6) µm. Asques octosporés, inertes dans l'iode. Paraphyses cylindriques, cloisonnées, légèrement renflées au sommet, remplies d'un pigment orange qui se colore en violet dans le réactif de Melzer. Boucles présentes. Poils à paroi épaisse, jusqu'à 300 µm, à base simple ou bifurquée (jamais multiple).

Lieu de récolte : Baisse de la Plate, 2600 m, Alpes-Maritimes (France), en terrain schisteux. Zone humide, au milieu des mousses.

Cordialement,
Edouard

Miguel Ángel Ribes, 05-08-2020 22:01

Re : Scutellinia cf. minor

Hi Edouard

Look at this link

https://www.dropbox.com/s/vtwrztbeeg3jkit/Contribucion%20al%20conocimiento%20de%20la%20micobiota%20del%20Parque%20Nacional%20de%20Ordesa%20I%20-%20Ribes%202016.pdf?dl=0

Edouard Evangelisti, 06-08-2020 19:54

Re : Scutellinia cf. minor

Hi Miguel,

Thanks for the link. It helps a lot! I summarize as follows:

- S. barlae has globose spores, while those of S. minor are subglobose.
- The boreoalpine distribution of S. minor is compatible with my collection.
- One discrepancy though: my collection does not have many bifurcate hairs.

I will consider getting an ITS sequence for this collection.

Best wishes,
Edouard

Viktorie Halasu, 06-08-2020 20:56

Re : Scutellinia cf. minor

Hello Edouard,

regarding the supposed boreoalpine distribution of S. minor - I'm aware that both Schumacher and Matocec listed it as such. However, both Sphaerospora minor and its synonym Sc. subglobispora were described from rather warm areas of the Czech Republic and certainly not higher then some 500-600 m a.s.l. I have even collected it in 220 m a.s.l., ash-poplar-oak forest near Olomouc. Also the collections known in neighbour Slovakia are mostly from colline and submontane level. In these two countries at least it doesn't seem to be restricted to high altitudes, unlike e.g. S. pilatii or S. mirabilis.
Might be interesting to compare sequences of colline vs. alpine collections. But I don't know which genes are useful in this genus.

Viktorie

Edouard Evangelisti, 07-08-2020 16:09

Re : Scutellinia cf. minor

Hi Viktorie,

Thanks for the information. I should mention mine was high in the mountains, but not cold at all. We are experiencing heat waves and the temperature, even at this altitude, was almost 30°C! I guess fungi could cope due to the small brook nearby and the reasonably cool nights.

I will check for the sequences most suited for barcoding and process the samples.

Edouard

Edouard Evangelisti, 28-07-2021 16:32

Re : Scutellinia cf. minor

Hello,

I just got a chance to obtain the ITS sequence of the one-year-old specimen mentioned above, which was either S. minor or S. barlae. My sequence is identical to JF908714 from the Venice Herbarium Project (collection from E. Campo, 1997, reference: this PloS One manuscript) . Thus, for people searching the forum, my collection should be considered Scutellinia barlae.

Cheers,

Edouard