Small Mollisia - Forum ASCOFrance

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Small Mollisia

Juuso Äikäs, 14-05-2020 20:18

Mollisia is a difficult genus, but I decided to post this and see if there is any hope of knowing the species.

These grew on a fallen, decorticated hardwood branch. The size of the apos is much smaller than of the typical gray ones that I find often on moist wood. There was a faint yellow reaction with 40% KOH, but under the microscope in 3% KOH I didn't notice anything.

Spore measurements: (7.9) 8.9 - 10.7 (10.8) × (2.9) 2.93 - 3.1 (3.2) µm, N = 8.

Hans-Otto Baral, 14-05-2020 20:23

Re : Small Mollisia

Did you view under the mic when the KOH reached the fungus? Simply mounting in KOH does not permit to see a reaction. 3% KOH would be enough, even 1% I think.

To state the apo diameter would be helpful too.

Juuso Äikäs, 16-05-2020 12:43

Re : Small Mollisia

The diameter of the larger ones is about 1/3 mm. I took some pieces on a microscope glass and put a cover slip on and then a drop of 3% KOH on the side (1st pic) and did the same with water (2nd pic). Looks a bit yellower in kOH, but nothing too dramatic.

Hans-Otto Baral, 16-05-2020 16:41

Re : Small Mollisia

Yes it is more yellow, but important is to view the instantaneous reaction. To photograph it needs rapidity. A possibility could be M. albogrisea ined., Phaeomollisia picea is a synonym but cannot be transferred to Mollisia because of Mollisia piceae (pers.) Quel. This species reacts yellow in KOH.

Juuso Äikäs, 16-05-2020 17:15

Re : Small Mollisia

Alright, thanks again for the info. Here's one pic: before and immediately after applying KOH.

Hans-Otto Baral, 16-05-2020 18:12

Re : Small Mollisia

So this looks negative.

Juuso Äikäs, 16-05-2020 20:53

Re : Small Mollisia

Maybe I'll stay away from Mollisias for a while :).

Mirek Gryc, 16-05-2020 21:14

Re : Small Mollisia

Hi Juuso
This is not a good idea.
Mollisia is difficult but among them you can find a lot of interesting species.

Do not cover the preparation with a slide when checking the reaction to KOH. Put a drop of KOH on the slide slide and then dip a piece of ascocarp in it. If the fruiting body reacts to KOH, you will see a yellowish coating around it in a few seconds.
The second way is to apply a drop of KOH to the fruiting body hymenium. If the reaction is positive, the hymenium will change to +/- yellow.
The reaction is visible to the naked eye or after applying a magnifier.
To be sure, it is worth using both methods at the same time
Good luck
Mirek

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