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éric ROMERO éric ROMERO

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Hi!We observed this hyphomycete growing between le

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Bonsoir à toutes et tous,J'ai trouvé ce matin ce

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Mollisiaceae sp. ?
Nicolas VAN VOOREN, 22-07-2008 00:37
Nicolas VAN VOORENJe sèche sur une espèce récoltée ce week-end dans la tourbière du Grand Leyat (Savoie), alt. 1660 m, sur branche morte d'Alnus viridis.
J'ai un peu cherché du côté des Mollisiaceae mais sans succès...

Macroscopical description: apothecia sessile, pulvinulate, at first yellowish then pale cream to whitish; outer surface furfuraceous, ochraceous. Ø 0.3–1.5 mm
No subiculum. Reaction to KOH 4%: none.
  • message #5186
Nicolas VAN VOOREN, 22-07-2008 00:47
Nicolas VAN VOOREN
Re:Mollisiaceae sp. ?
Microscopical features: asci 82-87 x 7-9 µm, with crozier, containing 8 sp. (± biseriate) ; pars sporifera as long as the ascus length; apical ring IKI bb.
Paraphyses sublanceolate, large to 3.2-4 µm, hyalin, septate, with a great VB in the upper part.
Spores fusiform elongated, 25-33 x (2.7) 3-3.2 µm, hyalin, with a lot of LB, and sometimes with a septum ("very mature" spores) but never in ascus.
Excipulum at the base of textura globulosa, with brown cells, Ø 8-20 µm, becoming more elongated on the lateral part.

Any suggestion?
  • message #5187
René Dougoud, 22-07-2008 07:07
Re:Mollisiaceae sp. ?
Bien Cher,

Je te réponds depuis le job et donc de mémoire, pour te dire que cela me fait penser à Mollisia ramealis !

Amitiés

René
Hans-Otto Baral, 22-07-2008 12:34
Hans-Otto Baral
Re:Mollisiaceae sp. ?
yes, I fully agree. Here images from Luzern, Sittlisalp, 1650 m, Alnus viridis, Aug. 2006.

In my opinion Dibeloniella citrinella is the same species. Figured in Schmid.Heckel 1 fig. 6

Zotto
  • message #5189
Hans-Otto Baral, 22-07-2008 12:34
Hans-Otto Baral
Re:Mollisiaceae sp. ?
Free living spores
  • message #5190
Hans-Otto Baral, 22-07-2008 12:36
Hans-Otto Baral
Re:Mollisiaceae sp. ?
margin in median section
  • message #5191
Nicolas VAN VOOREN, 22-07-2008 21:44
Nicolas VAN VOOREN
Re:Mollisiaceae sp. ?
Merci beaucoup.
Raúl Tena Lahoz, 24-07-2008 11:02
Raúl Tena Lahoz
Re:Mollisiaceae sp. ?
Spores of this species can be seen as these of my photo? Rest of features of my collection fit with those of Nicolas (except for colour, always yellowish). The explanation could be a fusion of the oil drops? Also spores like those Zotto sends are seen. Medium: distilled water.
Thanks!
  • message #5195
Hans-Otto Baral, 24-07-2008 12:16
Hans-Otto Baral
Re:Mollisiaceae sp. ?
Hi Raul

yes, in the free spores the LBs have fused and these spores are dead. There is a central small area which is unclear whether it is a septum or perhaps the nucleus.

Zotto
Raúl Tena Lahoz, 24-07-2008 12:38
Raúl Tena Lahoz
Re:Mollisiaceae sp. ?
Hi Zotto
I think that, in this case, it´s the nucleus. So better to use tap water? I don´t use it because I live in a very calcareous place, so I think it´s hard water. Maybe water of springs in acid soils could be better?
Thaks a lot!
¡Muchas gracias!
Hans-Otto Baral, 24-07-2008 12:43
Hans-Otto Baral
Re:Mollisiaceae sp. ?
I am sure it makes no difference to use distilled water, or hard tap water. Visible osmotic effects need higher concentrations. To see living spores depends on various matters: the fungus must be in good condition, it must be kept moist if it is xerointolerant as in the Mollisia here, you may only slightly press on the cover slip, and you may not apply heat or lethal chemicals.

Zotto
Raúl Tena Lahoz, 24-07-2008 12:52
Raúl Tena Lahoz
Re:Mollisiaceae sp. ?
Ok, Zotto. I wasn´t sure about that. Thank you for your advices.