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18-05-2024 10:47

Crocicreas cyathoideum?

Anna Klos

Goodmorning,We found this tiny ascomycete (max. 1m

17-05-2024 15:17

Ã©ric ROMERO

éric ROMERO

Bonjour, Un besoin d'aide pour ce Podospora (...?

17-05-2024 16:25

Erioscyphella lunata

Pavel Jiracek

Erioscyphella lunata, found on a fallen needle of

29-01-2022 21:44

Tiny yellow apothecia on soil crust

Jan Eckstein

Good evening, apothecia small, yellowish, 150-300

16-05-2024 11:19

Dasyscyphella claviculata ?

Sylvie Le Goff

Bonjour, j'ai récolté sur une branchette de feui

14-05-2024 09:19

Presentation about Orbilia

Hans-Otto Baral

Hans-Otto Baral

Hi, I want to announce for next Sunday 17.00 middl

16-05-2024 11:49

Una para ID 3818

Castillo Joseba

Castillo Joseba

De ayer en rama de abetoNo se por donde mirarA ver

14-05-2024 09:50

Yannick Mourgues

Yannick Mourgues

Hi. Found on decorticated wod of Populus cf. alba

16-05-2024 09:02

unknown 16-05-24

Vasileios Kaounas

Vasileios Kaounas

Found 15-05-24 in forest with Abies cephalonica, i

15-05-2024 17:26

Vasileios Kaounas

Vasileios Kaounas

First time I find this season and in this habitat,

Stictis radiata sur Fallopia japonica

Bernard CLESSE, 10-01-2016 22:31

Bernard CLESSE

Bonsoir à tous,

Suite et bientôt fin de mes récoltes sur renouée du Japon. Ici je pense à Stictis radiata, d'abord parce que les apothécies sont nettement plus petites (max. 0,3 mm de diamètre) que chez Stictis stellata (diamètre parfois dépassant le mm) montré tout récemment sur Fallopia japonica toujours et que d'autre part, les spores sont ici larges de 2-2,5 mu.

Qu'en pensez-vous ?

Bernard

Hans-Otto Baral, 11-01-2016 09:42

Hans-Otto Baral

Re : Stictis radiata sur Fallopia japonica

I recommend to make images in vital state though I am quite sure that the oil content in the spores is low. Also a section showing the periphyses at the inner face of the margin would be helpful, I remember Sherwood makes a difference based on their measurements. Did you notice any iodine reaction?

Bernard CLESSE, 11-01-2016 11:00

Bernard CLESSE

Re : Stictis radiata sur Fallopia japonica

Hi Zotto,

Thanks for your message !

I will try to make a cut of apothecium but I admit that I do not know what are the periphyses. Would you possibly a drawing or a microscopic picture so that I know what I have to observe? I will try to redo pictures spores alive.
Regarding the iodine reaction, I just noticed a very slight blue color and diffuses into the ascus (especially upper half) with lugol but not a color "deep blue" suitable for this species.

Bernard

Hans-Otto Baral, 11-01-2016 11:35

Hans-Otto Baral

Re : Stictis radiata sur Fallopia japonica

If you pretreat with KOh you will get a distinct blue of the whole hymenium, without KOH the reaction is first blue the red-brown, and only at the top the hymenium is bluish.

What is meant with periphysoids (not periphyses, sorry) you can see here.

Sherwoods distinction is perhaps a bit weird, and her opinion about amyloidity may be wrong as she appears not to have understood the influence of KOH and Melzer's.

But I must admit I have no clear concept of thse two species.

Bernard CLESSE, 11-01-2016 20:56

Bernard CLESSE

Re : Stictis radiata sur Fallopia japonica

Hi Zotto,

Thank you for your explanations !

Here are some new pictures :

a) hymenium+KOH+lugol : well blue !
b) ascus+lugol : dextrinoid; ascus cap : 3,5 mu thick
c) spores guttulated

What do you think about that ?

Bernard

Hans-Otto Baral, 11-01-2016 22:30

Hans-Otto Baral

Re : Stictis radiata sur Fallopia japonica

Oh look, these guttulate spores are exceptional, I don't know that from radiata or stellata. But I don't know these species well or can separate them.

A red reaction inside the ascus could be due to glycogen and would then be called dextriniod, but then it should be seen also after KOH, or in Melzer without KOH. What reacts blue after KOH (I think the ascus wall surface) must be red in IKI without KOH (hemiamyloid). See my homepage:
http://invivoveritas.de/articles/iodine-reaction-in-ascomycetes-why-is-lugols-solution-superior-to-melzers-reagent/
Fig. 2 and 5.