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06-04-2015 17:25

Enrique Rubio Enrique Rubio

Hi to all Few days ago I have found these small

07-04-2015 14:26

Blasco Rafael Blasco Rafael

Hola, tengo esta Rosellinia recogida sobre rama ca

06-04-2015 19:23

Alain BRISSARD

Bonsoir à tous Votre avis me sera précieux au s

31-03-2015 22:07

Garcia Susana

Hello: I found a group of apothecia growing on th

05-04-2015 19:46

Jenny Seawright Jenny Seawright

Hello all, Black disco found on thin dead twigs of

03-04-2015 18:48

Steve Clements

Cistella , Arachnopeziza?Found March 24 2015 on a

05-04-2015 16:40

Bernard CLESSE Bernard CLESSE

Un facile, sur branchettes de Tilia platyphyllos,

05-04-2015 10:42

Castillo Joseba Castillo Joseba

Tengo unos ejemplares de Trichoglossum que estan

01-04-2015 21:39

Bernard CLESSE Bernard CLESSE

Sur tranche de coupe de tronc complètement pourri

04-04-2015 12:26

Bernard CLESSE Bernard CLESSE

Une forme imparfaite d'un asco ou d'un hétérobas

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Help in the identification.
Lepista Zacarias, 18-09-2014 22:32
I thought that this one was a lichen, but it lacks an alga. It was growing on a shrub with smooth bark, in a calcareous habitat, in the margins of a small streamlet without water in summer.

Asci with 8 spores each; The spores are 1-septate when young and then develop other septa, up to 8-septate.
Asci: up to 215 x 12 um;
Spores:
(26.7) 29.2 - 33.7 (34.5) x (6.2) 6.5 - 7.9 (8.1) µm
Q = (3.9) 4 - 4.76 (4.8) ; N = 20
Me = 31.2 x 7.2 µm ; Qe = 4.4,


I will appreciate any hint for the classification.
Thanks in advance,
zaca

  • message #31333
  • message #31333
  • message #31333
  • message #31333
Hans-Otto Baral, 18-09-2014 22:39
Hans-Otto Baral
Re : Help in the identification.
Did you test the iodine reaction? I think it is a lichen with a whitish thallus.
Lepista Zacarias, 18-09-2014 22:44
Re : Help in the identification.
Thanks, Zotto, for your message.
In fact, I thought this was a lichen, but I found no trace of an alga.
The last two sets of photos (except for the  one on the left hand of the last) were taken after join a drop of melzer to the slide. This permits to see clearly the septation of the spores, which was not possible without it.
Thanks again,
zaca
Hans-Otto Baral, 18-09-2014 22:53
Hans-Otto Baral
Re : Help in the identification.
o.k., this would be interesting to look more closely. With oil immersion I am sure you will see the septa, but different. It seems the cells are ocellate, and the septum gets thicker when killed by MLZ.

It is well possible that the asci are inamyloid. Anyhow I would try Lugol for a possible hemiamyloid reaction.

The spores ar very characteristic, I think a lichenologist knows them.
Lepista Zacarias, 18-09-2014 23:08
Re : Help in the identification.
Dear, Zotto,
I will follow your suggestions and try a new preparation, whenever possible. If I'll find anything different, it will be reported here.
Thanks,
zaca
Alain GARDIENNET, 19-09-2014 07:24
Alain GARDIENNET
Re : Help in the identification.

Hi Zaca,


Like Zotto, I think it could be a lichen, with leprose thallus.


You can test chemical reactions on the thallus : K, P, C, KC.


How are ascomatas ? Perithecias ? Apothecias ?


Alain

Lepista Zacarias, 19-09-2014 20:40
Re : Help in the identification.
Problem with solution in sight: Strigula stigmatella.

The ascomata are perithecia. I made the spot tests and all chemical reactions were negative. Moreover, did new microscopic observation and found that there seems to be a brown or golden-green alga (perhaps of the genus Trentepohlia). I checked the reference mentioned below and, taking into account the microscopic data, found two genera of pyrenocarpous to which my specimen can belong: Porina or Strigula. I found, in particular, a species _Strigula stigmatella_ with similar features.
Many Thanks to Zotto and to Alain, for their comments that lead me to the possible solution,
zaca


Ref:
Alan Orange, British and Other Pyrenocarpous Lichens, 2013.
(available at: https://www.museumwales.ac.uk/media/13849/Orange,-A.-(2013)-British-and-other-pyrenocarpous-lichens.pdf)