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Louis DENY

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Hi, Could anyone help me with this Lophiostoma? 

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Ascocoryne cylichnium var cylichnium
Pérez del Amo Carlos Manuel, 16-12-2010 00:02
Pérez del Amo Carlos ManuelPongo datos de este Ascocoryne que encontramos hace unos días sobre madera de Pinus nigra.

Hemos seguido las claves de Baral y llegamos a A.cylichnium var cylichnium
Hans-Otto Baral, 16-12-2010 11:41
Hans-Otto Baral
Re:Ascocoryne cylichnium var cylichnium
Dear Carlos

Yes, it is typical A. cylichnium. Multiseptate overmature spores forming subglobose free conidia and crystals, and croziers are typical. The best character is only seen on a few of your images: vital spores are consistently multiguttulate, contrary to sarcoides and inflata.

you don't have it fresh?

Zotto
Pérez del Amo Carlos Manuel, 16-12-2010 19:08
Pérez del Amo Carlos Manuel
Re:Ascocoryne cylichnium var cylichnium
Hola y gracias por la ayuda Zotto

Ya no disponemos de material fresco, pero si de material deshidratado aunque no es muy abundante.

La fotos de microscopía si están hechas con material fresco.

Carlos
Hans-Otto Baral, 16-12-2010 21:33
Hans-Otto Baral
Re:Ascocoryne cylichnium var cylichnium
Hi Carlos

if you made the micros from fresh material you did not treat it well. :-/ The spore contents are strongly influenced by chemicals or whatever. Did you use pure water?

Zotto
Pérez del Amo Carlos Manuel, 16-12-2010 23:53
Pérez del Amo Carlos Manuel
Re:Ascocoryne cylichnium var cylichnium
Ya que la esporada no fué muy abundante están teñidas con Rojo Congo.
  • message #13727
Hans-Otto Baral, 17-12-2010 10:16
Hans-Otto Baral
Re:Ascocoryne cylichnium var cylichnium
That looks nice but does not represent the intact case. It totally obscures the difference to A. sarcoides (in Sarcoscypha you will have the same effect that the species cannot be distinguished). To study the mature living state you need living asci, and inside them you will see the multiguttulate non-septate spores. As soon as the spores get septa they are overmature. That you have large drops inside might come from the reagent, I do not known.

Zotto
Pérez del Amo Carlos Manuel, 17-12-2010 19:31
Pérez del Amo Carlos Manuel
Re:Ascocoryne cylichnium var cylichnium
Con agua
  • message #13729
Hans-Otto Baral, 17-12-2010 20:11
Hans-Otto Baral
Re:Ascocoryne cylichnium var cylichnium
Wuff!

From which part of Spain?

Zotto
Pérez del Amo Carlos Manuel, 17-12-2010 20:15
Pérez del Amo Carlos Manuel
Re:Ascocoryne cylichnium var cylichnium
Nalda
La Rioja
España