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19-04-2024 18:32

Anna Klos

Good evening,I found this Ascobolus on wet soil an

18-04-2024 18:52

éric ROMERO éric ROMERO

Bonjour, J'ai beoin d'éclairage(s) pour ce Daldi

19-04-2024 14:28

B Shelbourne B Shelbourne

Cudoniella tenuispora: Distinctive macro and habit

13-04-2024 11:44

Riet van Oosten Riet van Oosten

Hello, Found by Laurens van der Linde, April 2024

16-04-2024 17:43

Giovanni ANTOLA Giovanni ANTOLA

Bonjour,Trouvé sous paille humide, autour d'un je

14-04-2024 20:04

Manak Roman

Hi all,I have two very similar finding last weeken

17-04-2024 10:44

Bernard CLESSE Bernard CLESSE

Juste à côté du cône avec "Hyphodiscus ayelii"

16-04-2024 22:53

Bernard CLESSE Bernard CLESSE

Bonsoir à toutes et tous,Pourriez-vous m'aider à

14-04-2024 22:58

B Shelbourne B Shelbourne

• Bactridium flavum (anamorph): Distinctive macr

15-04-2024 14:37

Eric Rousseau

Bonjour,Je sais que les cyphelles ne sont pas des

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Helotiale on Ligustrum leaves
Enrique Rubio, 04-01-2023 19:33
Enrique RubioThese apothecioid ascomata are gregarious, superficial but perhaps erumpent, and colonise the underside of overwintered Ligustrum ovalifolium leaves. They are very exiguous and measure 100-200 microns in diameter; sessile, initially very shortly cupulate, later flattened to become convex and pulvinate and possess abundant and conspicuous excipular hairs with several septa, the base of which arises from a pigmented vesicular cell and the ends with an hyaline and obtuse apex. Their walls are not smooth but slightly roughened, as if encrusted, but not truly warty like those of the genus Cistella. The excípulum has an angularis or prismatica texture with angular or more or less rectangular cells, with the walls provided with a yellowish-brown pigment.
The 8-spored asci are clavate, with a base without croziers and a probable apical pore with no reaction in IKI. The ascospores are hyaline and smooth, cylindrical, with a fine guttular content. I envisage two genera for this ascomycete: Cistella and Psilachnum, although I am more inclined to the latter as the hairs do not have distinctly warty walls.
What do yo think?
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Hans-Otto Baral, 04-01-2023 20:43
Hans-Otto Baral
Re : Helotiale on Ligustrum leaves
Hi Enrique

my first idea is a member of Chaetomellales. I still have it as a family in the Helotiales. Chaetomella oblonga (= Zoellneria rosarum) might be similar but has much longer brown seta-like hairs.

Could you please send me your pics in higher resolution? Thanks.

Zotto
Enrique Rubio, 04-01-2023 20:53
Enrique Rubio
Re : Helotiale on Ligustrum leaves
Of course.Thanks to you.
Hans-Otto Baral, 04-01-2023 21:07
Hans-Otto Baral
Re : Helotiale on Ligustrum leaves
It has similarities to Z. rosarum indeed. Maybe a new species of Chaetomella. An ITS sequence surely would clarify this.
Enrique Rubio, 04-01-2023 21:21
Enrique Rubio
Re : Helotiale on Ligustrum leaves
But, as I said in the message, the apothecia do not have those brownish seta-like setae typical of Zoellneria (Cahetomella) rosarum.
Due to its very small size, it will be impossible for me to sequence this material.
Thank you, Zotto.

Hans-Otto Baral, 04-01-2023 21:48
Hans-Otto Baral
Re : Helotiale on Ligustrum leaves
That's a pity. I did not say that this is Z. rosarum. The close genus Pilidium is without hairs, and it would be interesting to explore how the hairs correlate with the sequences.

I looked my database and there is nothing on Ligustrum leaves.
Viktorie Halasu, 04-01-2023 22:52
Viktorie Halasu
Re : Helotiale on Ligustrum leaves
Hello Enrique, 
the fruitbody size might not be a problem. Check this paper - we used two different approaches to sequence the material, one traditional, the other (informally called "throw the fungus straight into the reaction") might be better suitable for this collection: http://www.czechmycology.org/_cmo/CM72207.pdf
Viktorie 

Enrique Rubio, 05-01-2023 09:35
Enrique Rubio
Re : Helotiale on Ligustrum leaves
Thank you very much, Zotto.
Hi Viktorie
I can't get your link to work. Can you send it to me so I can open it?
Hans-Otto Baral, 05-01-2023 10:40
Hans-Otto Baral
Re : Helotiale on Ligustrum leaves
I could immediately download the pdf and wonder why I did not have it although I mentioned it in regard to C. rufofusca in my sequence archive. Your sequences appear not to exist in GenBank, nor can I find them in UNITE/PLuto. It is funny to publish an article on genetic results and not to make the data freely available.

The method of placing apothecia directly in the PCR was already practiced by Bin Liu during the first phase of our molecular part of the Orbilia monograph about in 2009. But I cannot remember how often this method was applied in published papers on other discomycetes.
Viktorie Halasu, 05-01-2023 12:42
Viktorie Halasu
Re : Helotiale on Ligustrum leaves
Which sequences did you not find? I just checked all the accession nos. published in the supplem. material and all of them are public on genbank. Perhaps search by herbarium no. might be less straighforward because those were entered with space between herbarium code and number, so one has to use advanced search (in titles).
Hans-Otto Baral, 05-01-2023 13:01
Hans-Otto Baral
Re : Helotiale on Ligustrum leaves
Yes that works, e.g. with "PRM 953071". I do not have the supplement.