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Åge Oterhals

I found this pyrenomycetous fungi in pine forest o

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Hyaloscypha herbarum?
Zuzana Sochorová (Egertová), 09-11-2021 15:06
Zuzana Sochorová (Egertová)Hello,
I have here an ascomycete collected in Moravia, in elevation 220 m a.s.l., on strongly decayed stem of a broad-leaved tree (dominant species in the place are Populus tremula, Betula pendula and Alnus glutinosa). 
Ascospores hyaline, sometimes septate, (7.6) 9.7 - 12.1 (13.7) × (2.4) 2.7 - 3.1 (3.4) µm
Q = (2.5) 3.2 - 4.3 (5.4, Me = 10.7 × 2.9 µm; Qe = 3.7
Asci without croziers, IKI+, MLZ+, (49.3) 53.5 - 64.3 (73.7) × (7.1) 7.7 - 8.5 (8.9) µm
Q = (6.3) 6.33 - 8.4 (9.5), Me = 59 × 8.1 µm; Qe = 7.3.
Hairs are smooth.

Using Seppo Huhtinen´s key, I came to Hyaloscypha herbarum, although the asci are longer. Also the pattern of lipid bodies is different than I can see in Enrique Rubio´s photos.
Can somebody confirm H. herbarum or suggest a better option?
Thank you, Zuzana
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Zuzana Sochorová (Egertová), 09-11-2021 15:08
Zuzana Sochorová (Egertová)
Re : Hyaloscypha herbarum
more
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Hans-Otto Baral, 09-11-2021 20:28
Hans-Otto Baral
Re : Hyaloscypha herbarum?
Hi Zuzana

could you please provide a photo of the hairs? What I see in section looks more like a Cistella, but it is too small.

Zotto
Zuzana Sochorová (Egertová), 09-11-2021 21:33
Zuzana Sochorová (Egertová)
Re : Hyaloscypha herbarum?
Hi Zotto,
thanks, I cannot take photos today, hopefully tomorrow.
I must admit that I haven´t observed many hairs, they are rather inconspicuous.
But I can see in Raitviir 2004 that almost all species of Cistella have asci arising from croziers.
The only one with simple septa is C. grumosa (on fallen leaves of Acer) + in a few species it´s not indicated whether they have croziers. In my collection I am sure they are lacking.
Zuzana
Hans-Otto Baral, 09-11-2021 21:55
Hans-Otto Baral
Re : Hyaloscypha herbarum?
I agree, actually all on wood that I know have croziers. Anyway it would be good to see the shape and size of the hairs.
Zuzana Sochorová (Egertová), 10-11-2021 19:24
Zuzana Sochorová (Egertová)
Re : Hyaloscypha herbarum?
Now I have checked another part of the collection and the margin of apothecia look smooth. The margin is formed by elongated, smooth cells.
In the part from yesterday, there is another microscopic fungus growing on the wood between apothecia and also on them, and what I considered hairs were apparently belonged to this fungus.
Therefore, I must be in a wrong genus. Attached photos of paraphyses (hyaline, without VBs, not broadened at apex) and the margin.
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Hans-Otto Baral, 10-11-2021 21:14
Hans-Otto Baral
Re : Hyaloscypha herbarum?
Indeed, this makes it really difficult. Although Hyaloscypha may occur without hairs (former Parorbiliopsis), such even a little clavate hairs remind me more of Cistella.

Intriguing I find the allantoid spores. But as you say, Cistella without croziers seems impossible.

Zotto
Zuzana Sochorová (Egertová), 11-11-2021 19:53
Zuzana Sochorová (Egertová)
Re : Hyaloscypha herbarum?
Hmm, I felt already in the field I should better pretend I cannot see this fungus :-) Anyway, thank you for your help.