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Bonjour à tous,Je voudrais votre avis sur cette r

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De la pasada semana en rama posiblemente de hayaPi

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On Knopper gall, Moellerodiscus lentus?
Marian Jagers, 13-05-2020 22:59
Marian JagersCultured on a Knopper gall (Knopper gall found in a forest nearby Utrecht, The Netherlands).
I started a moist chamber the second week of February. After about 3-4 weeks, very small, hairy stalks emerged from the substrate. They slowely grew into long stalked apothecia.
With the exception of Mycena rhenana/M. Cecediophila, which I actually hoped to culture, I cannot find information about fungi growing on Knopper gall.

I think this is Moellerodiscus lentus, but the spores are bigger and the substrate is unknown. So I am still in doubt of this name is correct. Anyone want to help me?


Greetings Marian


Apothecia 0.5-3,2 mm in diam, 0.5- 14 mm high, long stipitate, arising scattered, completely hairy, beige (darker after rehydrating or after superficial damaging).
Receptacle young barely wider than the stipe, later wider and cup-shaped, flattening by age, beige to brown, margin beige.
Hymenium yellowish brown, brown to reddish brown.
Stipe 0,5 to 13 mm long, in the middle ± 0.5-1,2 mm wide, young more or less cylindrical, older broader above and tapering toward the base, more often curved, beige, sometimes brown blackish at the base.


Microscopic characters:
Apothecium without ionomidotic reaction.
Spores: smooth, hyaline, aseptate, 8-10(-11)x(3,5-)3,7x4,5 µm.
Asci: 8-spored, uniseriate or les often biseriate, 95-108x 7-8 µm, weakly J+ in Lugol, strongly J+ after treated with KOH, croziers present.
Paraphyses: equal to or slightly exceeding the asci, filiform, septate, branching, apex 2 -3 µm wide.
Subhymenium: consisting of vertically oriented, loosely interwoven hyphae, with light brown intracellulair pigment.
Medullary excipulum: consisting of interwoven hyphae, hyaline or with light brown intracellulair pigment.
Excipulum: mostly textura globulosa, consisting of thickwalled roundish to oval, sometimes angular cells, outer cells 6-13x6-12 µm, scattered chain-like and oriented perpendicularly to the surface.
Margin apothecium composed of clavate terminal cells, hyaline or with light brown intracellulair pigment, 25x5 µm.
Stipe outer cells textura globulosa (simular to the receptacle).


Literature with a key to the genus I used: Yan-Jie ZHAO. 2013. Taxonomic Study of Lambertella (Rutstroemiaceae, Helotiales) and Allied Substratal Stroma Forming Fungi from Japan. Dissertation to the Graduate School of Life and Environmental Sciences, the University of Tsukuba.
Literature with a key to the species and a description: Dumont, K.P. 1976. Sclerotiniaceae XI. On Moellerodiscus (= Ciboriopsis). Mycologia 68(2): 233-267.

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Hans-Otto Baral, 14-05-2020 09:44
Hans-Otto Baral
Re : On Knopper gall, Moellerodiscus lentus?
Hello Marian

what a detailed report! This is a Moellerodiscus and probably M. lentus.

Zotto
Raúl Tena Lahoz, 14-05-2020 16:33
Raúl Tena Lahoz
Re : On Knopper gall, Moellerodiscus lentus?
Hi Marian & Zotto
Nice description! But I feel it is not related to Moellerodiscus lentus (spores are not so wide), but to the species that grows on Eucalyptus. Of course, it is not the same species because it lacks of those beatiful yellowish VBs. Please compare to Enrique´s microphotos at Zotto´s Moellerodiscus folder "eucalypti H+ chlorin. Vbs".
Cheers,
Raúl
Hans-Otto Baral, 14-05-2020 16:55
Hans-Otto Baral
Re : On Knopper gall, Moellerodiscus lentus?
Hi Raul & Marian

you are both right, and excuse me Marian that I did not cautiously read your text - a time problem. I overlooked your statement about too wide spores. Indeed, they are too wide, but they would fit a sample of Moellerodiscus sp. on Quercus cupule which was in my main folder of the genus. I now created a Quercus folder for these two.

Zotto
Marian Jagers, 15-05-2020 10:29
Marian Jagers
Re : On Knopper gall, Moellerodiscus lentus?
Hallo Raúl and Zotto, thanks to both of you.

In between I contacted Stip Helleman. He will extract DNA from this collection.

Kind regards,

Marian
Hans-Otto Baral, 15-05-2020 10:35
Hans-Otto Baral
Re : On Knopper gall, Moellerodiscus lentus?
Ah this is good. I assume he does not do it himself :-)