From Aragüés del Puerto, Huesca, this rare species of 1-2.5 mm growing in an old lake filled, in the alpine floor at 2,230 m, between undetermined mosses, in a very shadow and cold place, collected the day 1/10/2019.

Macro reminds me a Boudiera, Marcelleina, etc., but I think it has none, although I guess it will not be a Discinella or Phaeohelotium, at least I do not fit with any species of these genera.

Ectal excipulum with prismatic-angularis to subglobose texture in the margin. Margin with chains of 3-4 subglobose cells. Medullar excipulum has a double structure: a more internal layer with textura intricata of quite thick hyphae and a more external layer with textura prismatica of very long and thick hyphae. Asci with 8 biseriate spores, IKI -, I believe that without croziers (at least I have not been able to see any clear), of (79.6) 80.5 - 89.5 (95.7) × (7.8) 8, 3-10.8 (11.2) µm; N = 22; Me = 85.8 × 9.1 µm. Cylindrical paraphysis, usually with 1 large BV in the apical cell. Ellipsoidal-subfusiform spores, with 2 LBs at the ends, covered by a gel around its perimeter or only at one end, of (9.3) 9.5 - 10.9 (11.6) × (3.7) 3.9 - 4.5 (4.7) µm; Q = (2.2) 2.3-2.7 (2.9); N = 41; Me = 10.3 × 4.1 µm; Qe = 2.5

Thanks in advance.

Hans-Otto Baral, 12-11-2019 10:23

Re : Discinella, Phaeohelotium ?? 011019

Hi Miguel-Angel.

the crystals and VBs are important, they suggest an Ombrophila in my opinion, although the medulla is hardly gelatinised. But I cannot remember a species with these characters. 

The terrestrical O. limosella and "Discinella" badicolor are similar but spores and amyloid asci are different.

Zotto

Miguel Ángel Ribes, 12-11-2019 10:38

Re : Discinella, Phaeohelotium ?? 011019

Hi Zotto

Interesting, I will check Ombrophila species.

Thanks a lot.

Miguel Ángel Ribes, 12-11-2019 12:28

Re : Discinella, Phaeohelotium ?? 011019

Hi again

Latin is not my favorite language, but I think this collection fits more or less with "Discinella" badicolor, with IKI - asci. Spores are a little smaller, but I think it is the nearest species:

https://www.biodiversitylibrary.org/page/43592441#page/204/mode/1up

Thank you Zotto.

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Hans-Otto Baral, 12-11-2019 16:22

Re : Discinella, Phaeohelotium ?? 011019

You may be right, but Boudier's smaller measurements leave some doubts, and whether his observation of inamyloid asci is due to inconspicuous apical rings remains to be clarified from the type.

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Miguel Ángel Ribes, 12-11-2019 22:57

Re : Discinella, Phaeohelotium ?? 011019

Yes, spore size is a problem, because Boudier always studied all his collections in vivo, didn't it?

To check iodine reaction in the Boudier's typus is another problem.

Thanks Zotto.

Hans-Otto Baral, 13-11-2019 07:30

Re : Discinella, Phaeohelotium ?? 011019

Boudier always worked on fresh specimens and his preparation method was obviously careful, but sometimes he illustrated dead elements, for instance Orbilia spores. Bt not here, especially that he saw so well the VBs in the living paraphyses is remarkable.

Miguel Ángel Ribes, 13-11-2019 08:42

Re : Discinella, Phaeohelotium ?? 011019

Ok, thanks Zotto and Marcel

So, Discinella aff. badicolor.

See you.

Hans-Otto Baral, 13-11-2019 08:45

Re : Discinella, Phaeohelotium ?? 011019

Hi Marcel
I never saw a drawing of Boudier that clearly originates from an exsiccatum. Perhaps he avoided making drawings of them?
Zotto

Hans-Otto Baral, 14-11-2019 09:48

Re : Discinella, Phaeohelotium ?? 011019

I have Boudier's paper but only pl. VI while I miss plate V. I assume you have it?