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Dead and dry inoperculate hemiamyloid 100318
Miguel Ángel Ribes,
28-08-2019 14:38
Hi friendsI studied this dry and dead inoperculate and hemiamyloid cup-shape fung, about 1 cm of diameter. Collected on 10/3/2018, at floor with needles of Pinus.
Asci cylindrical, 8-spored, hemiamyloid and with croziers.
Spores cylindrical to slightly alantoid, without LBs (dead state), (7,9) 8,7 - 10,6 (11,3) × (2,8) 3,1 - 3,7 (3,9) µm; Q = (2,2) 2,5 - 3,1 (3,8); N = 39; Me = 9,5 × 3,4 µm; Qe = 2,8. Spore edge looks like dextrinoid, it is slightly red, similar to spores of Ombrophila hemiamiloidea.
Paraphysis cylindrical without VBs.
Ectal excipulum with textura gelatinosa. Medullar excipulum with a textura mixed between angularis in the upper and prismatical in the lower part.
Thank you.
Hans-Otto Baral,
28-08-2019 15:30
Re : Dead and dry inoperculate hemiamyloid 100318
Hi Miguel-Angel
I assume the spores are without oil drops, but did you check that in KOH?
To be sure that VBs are absent seems difficult to me when only a dead specimen is at hand.
My only idea is a Discinella.
I assume it is from Spain?
I assume the spores are without oil drops, but did you check that in KOH?
To be sure that VBs are absent seems difficult to me when only a dead specimen is at hand.
My only idea is a Discinella.
I assume it is from Spain?
Miguel Ángel Ribes,
28-08-2019 16:58
Re : Dead and dry inoperculate hemiamyloid 100318
Hi Zotto
No, I didn't check the spores with KOH, because all the study is made with dead material. The table spores are with water and IKI. Do you think we can see any different character with KOH?
Sorry, it is from Canary Islands, Las Palmas de Gran Canaria.
Discinella? Perhaps a Phaeohelotium too?
Thanks a lot
No, I didn't check the spores with KOH, because all the study is made with dead material. The table spores are with water and IKI. Do you think we can see any different character with KOH?
Sorry, it is from Canary Islands, Las Palmas de Gran Canaria.
Discinella? Perhaps a Phaeohelotium too?
Thanks a lot
Hans-Otto Baral,
28-08-2019 17:26
Re : Dead and dry inoperculate hemiamyloid 100318
KOH is the medium for herbarium material :-)
In fact, oil drops are often masked in dead spores by the plasma but appear in full strength when mounting in KOH.
How old is the sample so that the spores are not further alive?
Phaeohelotium does not have such gelatinised excipulum.
In fact, oil drops are often masked in dead spores by the plasma but appear in full strength when mounting in KOH.
How old is the sample so that the spores are not further alive?
Phaeohelotium does not have such gelatinised excipulum.
Miguel Ángel Ribes,
28-08-2019 18:12
Re : Dead and dry inoperculate hemiamyloid 100318
Ok, I will try with KOH later, now I have another interesting asco alive.
It was collected at 10/3/2018.
Thanks.
It was collected at 10/3/2018.
Thanks.
Miguel Ángel Ribes,
02-09-2019 20:55
Re : Dead and dry inoperculate hemiamyloid 100318
Hi again
You were right, Zotto, the spores with KOH have oil drops, so Discinella sp. (Picture attached)
Thanks a lot.
You were right, Zotto, the spores with KOH have oil drops, so Discinella sp. (Picture attached)
Thanks a lot.
Hans-Otto Baral,
02-09-2019 21:22
Re : Dead and dry inoperculate hemiamyloid 100318
Indeed, now the genus is clear (species concepts are difficult and appear to require DNA).
Miguel Ángel Ribes,
02-09-2019 21:50
Re : Dead and dry inoperculate hemiamyloid 100318
Yes, will sequenced It.
Thanks again
Thanks again
Hans-Otto Baral,
02-09-2019 22:31
Re : Dead and dry inoperculate hemiamyloid 100318
Tell me the result, please.
Miguel Ángel Ribes,
02-09-2019 22:46
Re : Dead and dry inoperculate hemiamyloid 100318
Ok