Dear Forummembers

I've a problem with an asco on riched soil (flowerpot), sclerotia not observed.

Apothecium: 2- 8 mm diam.; 1- 6 mm high, first turbinate then turbinate to discoid narrowed  into a tapering stipe.; disc with a depression; pink brown; margin even and a little outwards reflexed; outside flocculose, dark pink brown.

Section:
-medular excipulum: elongated cells,  tended to textura epidermoidea
-ectal excipulum: textura subglobosa to textura angularis : outside with yellowbrown elongated excipulumcells; broadened at the top
Margin: transition between paraphyses and elongated excipulumcells:-7 septate ; cilindric,  subclavate, subcapitate; yellowbrown

Spores in water : 16-20(22)x(9)9.5 10 µm : smooth (also in cottonblue); eggutulate but small polair zone with diffuse droplets
Asci: 250-300x14-16 µm operculate; ascuswall amyloid in Melzers reagens;(ascustop didn't shows the typical Peziza darkblue top).
Paraphyses: agglutinated,cilindric;  6µm ; top broadened to-8 µm; light brown vacuolair pigment; -5 septate

Discussion:
Peziza fimeti: typical dark blue ascustop is missing , also without oildrops but different in medular excipulum? Does there exist a Peziza fimeti complex as a friend of my give me that suggestion?
Pseudombrophila-like but this genus is without amyloid ascuswall and asci < 200 µm
Pachyella: different hair outgrowths, spores with two oildrops

Thanks for any reaction.

Hans-Otto Baral, 03-09-2017 17:44

Re : Ascuswall amyloid genus uncertain

Hi Lieve
I am surprised about the obviously multinucleate spores containing at least 4 nuclei. I remember this as a rare feature in Pezizales, occurring in Sarcoscyphinae and perhaps a few other groups. In Syllabus I wrote for both Ascobolaceae and Pezizaceae the the spores were uninucleate.
Maybe there occur exceptions....
Zotto

Nicolas VAN VOOREN, 03-09-2017 18:22

Re : Ascuswall amyloid genus uncertain

This fungus reminds me Lepidotia hispida (sn. Peziza quelepidotia) because of the spore shape and the spore content (and also the amyloid reaction of asci) and the presence of a stipe, but there are some differences:
- color of apothecia;
- size of ascospores;
- pattern of amyloid reaction;
- asci with croziers.

No idea of what it can be, maybe an exotic species...

Lieve Deceuninck, 04-09-2017 09:52

Re : Ascuswall amyloid genus uncertain

Hi Zotto and Nicolas
Thanks for your interesting addtions and info.

Lieve