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02-08-2025 12:35

éric ROMERO éric ROMERO

Bonjour tous, J'ai trouvé sur pétiole de Fraxin

02-08-2025 17:21

Andgelo Mombert Andgelo Mombert

Bonjour,Je recherche la description et la planche

30-07-2025 20:52

Bohan Jia

Hi together,  I've been looking posts in AscoFra

30-07-2025 10:12

Bernard CLESSE Bernard CLESSE

Bonjour à toutes et tous,Pourriez-vous m'aider à

31-07-2025 16:32

Andreas Gminder Andreas Gminder

Dear collegues,today I found on a very fresh fire

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Stefan Jakobsson

On a decorticated twig of Alnus incana on moist so

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Castillo Joseba Castillo Joseba

Me mandan el  material seco de Galicia, España,

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Enrique Rubio Enrique Rubio

Hi to everybody.I would appreciate any ideas about

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Richard Droker

Growing on resin (over moss on Tsuga heterophyll)l

13-06-2025 09:41

Josep Torres Josep Torres

Hello.A cerebriform ascomycete sprouting scattered

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Eutypa lejoplaca?
Garcia Susana, 23-01-2014 14:32
Hi all

I found this Pyrenomycete, growing on wood of Acer campestre.
Perithecia size is 200-260um in diameter. Ostioles are 115-140um in diameter and have a cruciform shape.
Asci measured 55-68 × 5-7um, area spores 15-23 × 5-6.5um. With IKI, the apical apparatus becomes more apparent, but I'm not able to say if it is IKI +. in any case would be weakly positive.
Persistent paraphyses.

Following keys Rappaz, came to Eutypa Lejoplaca but I doubt if it could be another species, such as E. maura, or leptoplaca (if I consider the reaction to IKI is +).


Can anyone identify the species with the data provided?


Thank you,


Susana (Spain)

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Jacques Fournier, 23-01-2014 17:49
Jacques Fournier
Re : Eutypa lejoplaca?
Hola Susana,
you are right, your fungus is likely around E. leptoplaca and E. lejoplaca. Eutypa maura can be ruled out because it is distinctive in having more immersed perithecia and the wood markedly blackened beneath the surface between the necks.
The most reliable difference between the two species is the reaction of the apical apparatus to iodine.
I suggest you follow Rappaz's procedure with a short pretreatment of perithecial content in 3% KOH and mounting in Melzer's reagent. This leads to a more drastic retraction of the ascal content than IKI and makes the apical apparatus easier to make out. Remember it is in fact subapical, at the bottom of a shallow tubular structure. The thing is to work on a very small amount of material in order to make a very thin slide, otherwise interpreting the iodine reaction is most often tricky, especially in species where the apical apparatus is very small. This applies to all species of Diatrypaceae since the character of reaction to iodine is crucial.
Come back with a clear result, whatever how long it takes!
Cheers,
Jacques
Garcia Susana, 23-01-2014 21:50
Re : Eutypa lejoplaca?
Salut Jacques

¿C'est la réaction tout aussi intense avec KOH + Melzer, qui KOH + IKI?


J'ai fait quelques préparations KOH + IKI (je n'ai pas de Melzer) et pense que c'est positif, j'observe dans les asques un couple de très petits points que je dirais sont bleus.


Que pensez-vous?


Saludos


Susana

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Jacques Fournier, 24-01-2014 08:56
Jacques Fournier
Re : Eutypa lejoplaca?
Hola Susana,
ton excellente photo montre bien une réaction positive, amyloide. Tu peux donc écarter lejoplaca.
E. leptoplaca est beaucoup plus fréquente sur Fraxinus, tu devrais la trouver facilement sur des petites branches tombées récemment avec le vent et tu pourras ainsi lui comparer ta récolte.
Amitiés,
Jacques
Garcia Susana, 24-01-2014 11:04
Re : Eutypa lejoplaca?
Muchas gracias Jacques

Saludos.
Susana