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07-06-2025 15:39

Edvin Johannesen Edvin Johannesen

A friend sent me a few Betula seeds with tiny blac

08-06-2025 18:03

éric ROMERO éric ROMERO

Bonjour à tous, Une récolte alpestre discrète

08-06-2025 14:55

Edvin Johannesen Edvin Johannesen

Ascomata only ca. 1 mm, erumpent on very thin Sali

06-06-2025 12:12

Hans-Otto Baral Hans-Otto Baral

Dear all I want to ask you if you have any recent

04-06-2025 15:10

Stefan Jakobsson

Hi forum, On a herbaceous stem, possibly Aegopodi

03-06-2025 20:52

Stefan Blaser

Hello everybody, I didn't get anywhere with this

04-06-2025 14:02

Matthew Rozanoff

Good morning,I was wondering if anyone recognizes

01-06-2025 09:37

Charles Aron Charles Aron

Hi All, I found this Octospora growing with liver

30-05-2025 10:20

Elisabeth Stöckli

Bonjour, Sur branches mortes de Salix en place (m

20-05-2025 22:15

Francois Guay Francois Guay

I found this ascomycete at the base of a dead fern

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'discomycete' on Dryopteris dilatata
Chris Yeates, 16-10-2011 03:52
Chris YeatesCollected on a dead attached rachis of Dryopteris dilatata at 255 metres above sea level in the Yorkshire Pennines, England. The encrusted excipular margins and strongly dextrinoid paraphysis tips are very distinctive, but I have drawn a blank: this seems not to be among any of the Helotiales recorded on Dryopteris in the UK.

Apothecia flat with slightly raised rims, sessile, up to circa 400µm diameter; dull brown with darker margins.
Excipulum from basal hyaline textura globulosa/prismatica to a margin heavily encrusted with dark brown material.


Asci 8-spored 48-60 x 6-8µm, pore blueing in IKI;ascospores elliptic-fusiform 9.75-10.5 x 3µm.


Paraphyses with rather thick-walled apices which are dextrinoid and variously clavate/obpyriform/capitate in shape.


Any suggestions very much appreciated .


Chris

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Hans-Otto Baral, 16-10-2011 09:43
Hans-Otto Baral
Re : 'discomycete' on Dryopteris dilatata
Salut Chris

the paraphyses are not thick-walled but they contain a refractive vacuole in the inflated apex. You did not see them in water? It would be intersting whether they were hyaline or perhaps yellow.

This is clearly a "Crustomollisia", a genus which can either be talen as a synonym of Calycellina, or treated as Calloriella or Micropeziza p.pt. I presently prefer to say Calloriella.

I have here species on herbaceous stems (C. umbrinella  = Allophylaria soederholmii), Quercus leaves (c. castanea = Crustom. roburnea), Cyperaceae/Poaceae (Pseudonaevia caricina ?= Actinoscypha muelleri; Micropeziza cornea/karstenii), and some others, but none on ferns.

You see from this that it is a rather confused though very interesting group, with these brown clods of exudate on the exterior.

I attach here a photo of C. castanea with yellow VBs in the paraphyses.

If there is anything in the literature that fits to your fungus I will see in my database.

Zotto
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Hans-Otto Baral, 16-10-2011 09:48
Hans-Otto Baral
Re : 'discomycete' on Dryopteris dilatata
The only thing I got was Allophylaria filicum, but that is a hyaline fungus. Likewise is Pezizella campanuliformis.

If you could get some free spores (I assume it is not easy) then I could better see the contents. I think the spores are aseptate and contain larger and smaller oil drops.

The VBs you can only see in the living paraphyses. f you see them, you can try also Cresyl Blue for staining them. If there are any spores around, please check if the surface stains lilac in CRB.


Zotto
Chris Yeates, 17-10-2011 23:14
Chris Yeates
Re : 'discomycete' on Dryopteris dilatata
many thanks Zotto - I don't feel so bad about being confused by this one now!

I am keeping it damp for a day or so to ensure ripe spores, I am pretty certain they are non-septate though; I don't have cresyl blue, but Derek Shafer has put me on to a UK supplier who sells it in dry form as Brilliant Cresyl Blue; some places sell it as a solution - the formula being (this I understand is sold for diagnostic blood testing) 1% Cresyl Blue and 0.85% sodium chloride in water?

is the NaCl actually needed?

thanks

Chris
Hans-Otto Baral, 18-10-2011 18:11
Hans-Otto Baral
Re : 'discomycete' on Dryopteris dilatata
Yes, about 1% is good, but it is simply enough to create a blue solution which is added to a water moutn where it is diluted.

No NaCl necessary, this is, was you say, only for cells that do not have a cell wall. In fungi you would provoke a certain shrinkage of the cell because you take away the turgor.

Zotto

Chris Yeates, 21-10-2011 00:14
Chris Yeates
Re : 'discomycete' on Dryopteris dilatata
Hello Zotto (and others)

Yes, it has proved difficult to get free spores; see the accompanying image, the spores looked very similar to those in the asci being almost full of oil drops - I did however see a single spore (pictured - hidden beneath "PREV"!) which looks unequivocably septate; the VB's in the paraphysis tips are indeed pale yellowish (rather as in your image) . . . . all these are of living material in water
the excipulum is almost entirely covered in exudate as can be seen in the other photograph; this does not match at all well with a description of Pezizella roburnea in TBMS 67(3) page 481:
"margin formed of compacted slightly pigmented pseudo-parenchymatous cells"; I do not have access to Velenovsky's original description. Also the habitat is very different - am I correct in thinking there are not many ascomycete species which occur on both Pteridophyte and Spermatophyte plants? I know a few fern rusts alternate with Gymnosperms but that is a different matter

best wishes

Chris


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Hans-Otto Baral, 21-10-2011 08:28
Hans-Otto Baral
Re : 'discomycete' on Dryopteris dilatata
Hi Chris

I attach here the paper of Svrcek where you see the brown patsches on the excipulum. Don't know what Hawksworth & Sivanesan saw (on Castanea leaves).

The high lipid content in your spores clearly excludes Calloriella castanea = Crustomollisia roburnea. I have a collection on Vaccinium stems from lapland which resembles your fungus also in the spores, but it was not very wel developed and remained unidentified. Spores were ca. *14-15 x 5-5.5 µm?, so yours are smaller.

Did you measure the free spores? They have a sheath which is typical of Calloriella. The septum is probably only present at an overmature stage.

Maybe it is an undescribed species, I found nothing on ferns in my database.

Zotto