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13-06-2025 16:34

Andgelo Mombert Andgelo Mombert

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13-06-2025 19:57

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12-06-2025 19:36

Rodríguez Borja Rodríguez Borja

Hi, I have got this collection hoping you can giv

13-06-2025 09:41

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Angel Pintos Angel Pintos

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11-06-2025 13:44

Nicolas Suberbielle Nicolas Suberbielle

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Peter Welt Peter Welt

An Ascobolus species for which I can't find a name

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Lachnum sp. on Larix sp. cones
Rodríguez Borja, 12-06-2025 19:36
Rodríguez Borja
Hi,

I have got this collection hoping you can give me advise..

Habitat: Apex of several Larix sp. cones, after one week in a moist chamber. Monte Xalo, A Coruña, Galicia. 01-06-2025.

Description:

Cupulate ascocarps up to 1.1 mm in diameter and up to 1.25 mm tall, including the stipe. Pure white in color, covered with abundant water droplets (guttules) on both the stipe and the apothecial margin. Guttules are also visible in the center of the disc. Under 40× magnification, hairs can be observed both along the margin of the apothecium and along the stipe.

Microscopically, it is characterized by narrow, ellipsoid spores measuring (6.8) 7.4–8.2 × (1.7) 2.0–2.3 µm; Q = (3.4) 3.7–4.2; N = 32; C = 68%; Me = 7.5 × 2.0 µm; Qe = 3.8. The spores generally have acute ends, and in some cases are slightly curved, boomerang-shaped. Asci are up to 50 × 6–8 µm, amyloid (IKI+), 8-spored, with croziers at the base. Paraphyses are lanceolate, up to 80 µm long and 10 µm wide, with a basal and an apical septum, and no visible refractive vacuolar bodies (VBs). The apothecium is covered by an outer layer of cylindrical hyphae arranged in a palisade parenchyma; the terminal cells of these hyphae differentiate into filiform hairs: capitate, hyaline, with 2–3 septa, slightly thick-walled, up to 70 × 6–8 µm. These hairs are shorter on the stipe, where they reach up to 50 µm in length.

Observations:

Preliminary identification was attempted using Baral's Key to European species of Lachnum (XXX), but this collection doesn't quite match any of the described species, the closest may be Lachnum subvirgineum. I have seen the collection described by my friend M.A. Ribes (2009) in Contribución al conocimiento de la micobiota de las Islas Canarias (España) I (Bol. Soc. Micol. Madrid 33: 201–223) on this species although in his collection the spores were broader. I also reviewed Suková's (2005) work (A revision of selected material of lignicolous Lachnum species from the Czech Republic with a note on graminicolous material of the Lachnum pygmaeum complex, Czech Mycol. 57(3–4): 183–213), but couldn't find any species that matches this one either. At one point in her paper I considered Lachnum pygmaeum, described from Picea, but that species typically darkens when dry, which is not the case here. Moreover, the spore dimensions and apothecial features don't fully align.

At this point, I suspect this could belong to the Lachnum subvirgineum complex.

**Note: The study was done on living material yesterday, and water was used for the observations. Scale bar 10 microns. I have additional material available if anyone would like to continue the research...


 


What do you think? Thank you in advance!!


 


Best regards,


Borja

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Rodríguez Borja, 12-06-2025 19:41
Rodríguez Borja
Re : Lachnum sp. on Larix sp. cones
Forgot something.. :)
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Hans-Otto Baral, 12-06-2025 22:16
Hans-Otto Baral
Re : Lachnum sp. on Larix sp. cones
You are fully right with your detailed presentation, and it may be the best to speak of a complex as long as we do not know it better. In a recent paper in Boletus, with an English version downloadable:


you can find a phylotree in which L. virgineum and L. cf. subvirgineum are identified as different well supported clades, the latter with a cf. because morphological docus were only available for one of these sequences.

I think we need a thorough DNA study on this complex to clarify how to circumscribe L. subvirgineum. 

L. pygmaeum is similarly unclear.
Rodríguez Borja, 13-06-2025 16:32
Rodríguez Borja
Re : Lachnum sp. on Larix sp. cones
Dear Zotto,

Thank you very much for your insightful comment, and for sharing your publication, I wasn't aware of your work on this genus, interesting!


As you rightly pointed out, morphological data alone may not be sufficient...


I've preserved material for future studies in case someone wants to work on this complex in the future.


Herbarium:BR 540. Lachnum subvirgineum complex
Collected on the apex of Larix sp. cones
Monte Xalo, A Coruña, Spain
Coordinates: 43.2329444, -8.4092222
Date: 1 June 2025
Leg: B. Rodríguez-de-Francisco
iNaturalist: https://www.inaturalist.org/observations/289265283
AscoFrance: http://www.ascofrance.com/forum?page=1&id=82905


 


Best regards,


Borja