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Nicolas VAN VOOREN
Hello, bonjour.Here is the paper I'm searching for
Small white discomycete
Francois Guay,
15-04-2025 15:06
I found this small white disco on decaying deciduous wood in Québec, Canada.•Asci: 40.8–45.1 × 4.1–5.4 µm, IKI+
•Some paraphyses seem to be IKI+; they turn all blue
•Spores: 5.3–7.3 × 1.9–2.7 µm
Here is the iNat link for photos: https://www.inaturalist.ca/observations/270017818
Any ideas on what it could be?
Thank you
François
Thank you
François
Hans-Otto Baral,
15-04-2025 16:24
Re : Small white discomycete
Interesting! Such amyloid vacuoles exist, for instence in Rutstroemia alnobetulae as I remember. But in such a hyaloscyphoid species I am surprized. Perhaps the paraphyses must be alive in order to react? Is the reaction also in MLZ?
Francois Guay,
15-04-2025 19:01
Re : Small white discomycete
Thank you, Zotto. Yes, the specimens were alive. I have not tried MLZ, but I will do so today and keep you updated. I will also send some specimens for sequencing.
Francois Guay,
15-04-2025 22:31
Re : Small white discomycete
Hi Zotto,
I did the microscopy in MLZ and found no amyloid reaction in the paraphyses. I tried again with IKI and got some positive reaction; some paraphyses are turning blue, though not all of them.
Looking at the specimens again macroscopically, I noticed that some have a small pseudostipe with a blackish coloration. Do you have any idea what genus this could belong to?
Thanks
François
Thanks
François
Hans-Otto Baral,
16-04-2025 07:59
Re : Small white discomycete
I tend to assume a Pezizellaceae because of the VBs though only faintly refractive. Lack of hairs contradict a Ciliolarina. Macroscopically I also thought a Leptodontidium which is another family (Leptodontidiaceae).
Francois Guay,
17-04-2025 00:04
Re : Small white discomycete
Very interesting, Thank you Zotto, I will update this post when I get the DNA sequence!