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Peziza sp.
Luc Lenaerts, 17-05-2023 14:59
Luc LenaertsHello,
On the 11th of May 2023 I found in Hengelhoef, Houthalen (Belgium), in forest, this Peziza:
Length about 5-6 cm, width 3-4 cm, hymenium according to Standard Soil Color Charts 7.5 YR 4/6 brown and 5/6 bright brown; exterior much paler and soon appearing grey-white; no juice present when injured; growing near beech and oak, on the soil or on small twigs; spores with small warts, < 1 µm high, not apiculate, in water with 2 large oil drops; spores in water: (16.0-) 16.8-18.4 (-19.2) x (8.2-) 8.9-10.3 (-11.0) µm, Q= (1.6-) 1.7-1.9 (-2.0), spores in coton blue: (15.5-) 16.4-18.2 (-19.1) x (8.3-) 8.8-9.8 (-10.3) µm, Q= (1.7-) 1.8-2.0 (-2.1); asci Melzer +, paraphyses simple, with small club-shaped thickening on top, with colourless content, not moniliform; excipulum: layered with different types of layers (hyphae, round cells, some angular cells.
Seems to be Peziza alvernensis, except the presence of 2 oil drops in all spores; an other possibility is Peziza domiciliana but I haven't any idea about the excipulum of this Peziza. The spores are too small for Peziza emileia and too big fot Peziza domiciliana.
Who can help?
Luc Lenaerts
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Nicolas VAN VOOREN, 17-05-2023 15:37
Nicolas VAN VOOREN
Re : Peziza sp.
These two "guttules" are strange indeed.
The spore cize and ornamentation fit to P. arvernensis, but the latter shows ascospores with bipolar spore granules (in livng state). Maybe your spores are dead and these BSG have merged into these large bodies... Can you check this point with mature ascospores present in asci or just ejected?

I have no experience of the true P. domiciliana, but this species should have smaller ascospores. But why not...
Peziza emileia is excluded here, it doesn't belong to Peziza s. str.
Luc Lenaerts, 17-05-2023 18:04
Luc Lenaerts
Re : Peziza sp.
Thanks for the logical explanation of dead spores.
The granules were not present in all spores.
Luc Lenaerts
Nicolas VAN VOOREN, 18-05-2023 11:52
Nicolas VAN VOOREN
Re : Peziza sp.
Do you think possible to show some asci containing living spores?
Luc Lenaerts, 19-05-2023 09:37
Luc Lenaerts
Re : Peziza sp.
Unfortunately, the material has dried.
I only have a few other pictures of the asci in Melzer.
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Mirek Gryc, 20-05-2023 08:55
Re : Peziza sp.
Hi

In my opinion, the spore content is suitable for Peziza arvernensis.

Mirek