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18-07-2025 23:03

Josep Torres Josep Torres

Hello.Fruitings between 51 and 130 microns in tota

17-07-2025 11:55

Castillo Joseba Castillo Joseba

De ayer en bosque de hayas y abetos, en tieraEjemp

16-07-2025 17:34

Bernard Declercq Bernard Declercq

Hello,I have trouble distinguishing above mention

14-07-2025 11:20

Michel Hairaud Michel Hairaud

Bonjour, Voici une espèce de  (?) Hyaloscyphace

15-07-2025 13:27

Angel Pintos Angel Pintos

Hello, does anyone have access to the following ar

16-01-2023 21:31

Riet van Oosten Riet van Oosten

Hello, Nearby the find of Calycina claroflava on

14-07-2025 17:55

Yanick BOULANGER

BonjourAutre dossier laissé en suspendJe viens de

14-07-2025 11:17

Yanick BOULANGER

BonjourJ'ai un dossier Jackrogersella qui est rest

14-07-2025 15:52

Gernot Friebes

Hi,I wanted to share this collection on Rubus idae

14-07-2025 13:37

Gernot Friebes

Hi,do you think this collection could be R. ulmari

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Hymenoscyphus sp.
William de Jong, 13-11-2020 14:14
Hi, 
I'm quite new in working with a microscope. Can anyone help me with the identification of this Hymenoscyphus-species? Location: The Netherlands. Substrate a decaying herbacious stem, maybe Rubus stem, but certainly not woody. Asci: 90-115 micrometer long. Spores: 16,6-20,5 x 4,6-5,6 micrometer, measured 6 spores, average: 18,8 x 5,2 micrometer.
Thanks in advance, kind regards,
William
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Hans-Otto Baral, 13-11-2020 16:28
Hans-Otto Baral
Re : Hymenoscyphus sp.
Hi William

it is not an easy way to learn microscopy, mainly making good preparations. And it is another difficulty to study living cells. Your collection is perhaps overmature and largely dead, it seems to me.

Hymenoscyphus seems right but the magnification (oil immersion?) could be better.

Start looking for Hymenoscyphus scutula, which is a variable species or aggregate, including e.g. H. vitellinus.

Do you use my folders?


Go to Ascomycetes illustrations, 7f Helotiales, 5a Helotiaceae

Zotto
William de Jong, 13-11-2020 19:51
Re : Hymenoscyphus sp.
Thank you very much for your reply, Hans-Otto / Zotto! I found this Hymenoscyphus in my garden, so I wanted to give it a try. This material was indeed already 5 days old after I got the chance to watch it under the microscope... That's probably why the asci are so wrinkled?? 

I worked the last weeks with rust fungi, for which it doesn't matter to dry out. So for Hymenoscyphus (and probably most fungi?) I have to use fresh material. Probably there is still some material in my garden, I will have a look.

But maybe I should work with more easy stuff first. Any suggestions?

The photo's are taken with a smartphone, a microscope camera is on it's way.

Thank you for showing me your folders, that's a lot of very interesting info/pictures!!
Hans-Otto Baral, 13-11-2020 20:32
Hans-Otto Baral
Re : Hymenoscyphus sp.
Did you let it dry during these 5 days? That would explain it.

Hymenoscyphus is not a bad fungus to start. But the excipulum is a bit tough, so it is not easy to carefully squash which is necessary to keep the cells alive. Any stronger pressure kills them. Good is to try a section with a razor blade but whith such thin-stalked apos this is difficult. Try with a needle to take out a small amount of hymenium for a water preparation and do not apply any pressure on the coverslip.

Rusts surely also belong to the living fungi :-)  Similar as lichens. But their spores probably survive drying for a reasonable time.

Characters of the living cells have been neglected in all groups of fungi.
William de Jong, 16-11-2020 13:06
Re : Hymenoscyphus sp.
Unfortunately, I couldn't find any of this Hymenoscyphus anymore (in my garden). But good to know how to handle it next time. Thank you very much!