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15-11-2020 18:32

Andgelo Mombert Andgelo Mombert

Bonsoir, Une bien jolie espèce découverte sous

07-11-2020 15:05

Andgelo Mombert Andgelo Mombert

Bonsoir, Sur branche morte de Acer campestre en s

12-11-2020 01:10

Yannick Mourgues Yannick Mourgues

Trouvé sur bois mort très dégradé d'Abies alba

12-11-2020 09:47

Hans-Otto Baral Hans-Otto Baral

Hello Since about a week the Orbilia monograph ca

14-11-2020 10:31

Castillo Joseba Castillo Joseba

Me mandan el marterial seco, recolectado en hoja d

13-11-2020 20:08

Pascal RIBOLLET

Bonsoir, Petits périthèces orange fixés sur un

14-11-2020 10:32

Castillo Joseba Castillo Joseba

Me mandan el marterial seco, recolectado en hoja d

14-11-2020 10:32

Castillo Joseba Castillo Joseba

Me mandan el marterial seco, recolectado en hoja d

14-11-2020 10:32

Castillo Joseba Castillo Joseba

Me mandan el marterial seco, recolectado en hoja d

14-11-2020 10:31

Castillo Joseba Castillo Joseba

Me mandan el marterial seco, recolectado en hoja d

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Asco on soil
Robin Isaksson, 19-10-2020 22:04
Robin IsakssonFound on soil sling with mosses.

Asci lugols negative. Spores 11-14x5-7 um
Have no clue where to look.
Somehe knows it?

Regards
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Hans-Otto Baral, 19-10-2020 22:17
Hans-Otto Baral
Re : Asco on soil
Uff, could you send more pics of the spores? And make a test: do the drops in the spores disappear in KOH?

This sounds like a Bryorbilia (Orbiliomycetes), a genus for which only one collection is known so far!

Is this a very sparse sample?
Robin Isaksson, 19-10-2020 22:54
Robin Isaksson
Re : Asco on soil
Hi!

Here come pictures of the spores. Sorry about quality.

It seems like the drops remains in koh 10%.
Last picture in koh.
Tested koh+lugols too now and it is no reaction in asci.

There is around 10 apotecia in the collection, i can collect moore because it was plenty of it in the slope.
But it has been cold now 3 days with minus degrees.


Regards
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Hans-Otto Baral, 20-10-2020 08:43
Hans-Otto Baral
Re : Asco on soil
Ah, this would be good if you collect further apos. Please dry them carefully and send them to someone who does sequencing, perhaps Guy Marson. From where is the sample, is the geology acidic?
Robin Isaksson, 20-10-2020 20:42
Robin Isaksson
Re : Asco on soil
Hi,

ok i will collect more on friday.
Its from Sweden, provins Småland. was found on a pile of earth but I have a hard time believing that there would be lime in the ground because everything around was silicate rocks sticking up. we have very little land with lime where I live. so yes its acidic.

I am happy to send a collection to someone who wants it for sequencing. do you have an email to some?


all the best
Hans-Otto Baral, 20-10-2020 20:55
Hans-Otto Baral
Re : Asco on soil
O.k., thanks! The only Bryorbilia collection so far known was also on silicate but from Asturias.

I hope I can tell you soon an address.
Hans-Otto Baral, 21-10-2020 11:00
Hans-Otto Baral
Re : Asco on soil
Yes, Guy would like to sequence it. Please let me know your e-mail then I send you his address.

Guy says: please not as registered mail - and the material should be dried beforehand on a "small heat source" (e.g. near the transformer of an electrical appliance or on a radiator).
Robin Isaksson, 21-10-2020 21:07
Robin Isaksson
Re : Asco on soil
hi,

Great!

robinisaksson69(at)gmail.com 

Hans-Otto Baral, 10-11-2020 20:58
Hans-Otto Baral
Re : Asco on soil
For all a good news: sequencing was successful and this species can be assigned to Bryorbilia arenicola, though with 8 nt deviation in the ITS1 but none in ITS2 and LSU D1-D3 (ITS distance 1-1.2%). Unnamed environmental strains of Bryorbilia spp. and other genera are very difficult to align in the ITS and differ by min. 8% in the LSU.
Robin Isaksson, 10-11-2020 22:25
Robin Isaksson
Re : Asco on soil
Thank you for the help with this one!


Regards,

Robin