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Mot de passe perdu? S'inscrire

17-09-2025 19:43

Philippe PELLICIER

Sur branche morte de Mélèze. Les ascospores sphÃ

17-09-2025 16:14

Philippe PELLICIER

Apothécies enterrées, fermées au début puis s'

17-09-2025 10:50

Heather Merrylees

Hi there!I am hoping for any advice on the identif

11-09-2025 16:57

Jason Karakehian Jason Karakehian

Our revision of Marthamycetales (Leotiomycetes) is

16-09-2025 12:53

Philippe PELLICIER

Pézizes de 1-4 mm, brun grisâtres, sur les capsu

03-09-2025 12:44

Enrique Rubio Enrique Rubio

Hi to somebody.I would like to know your opinion o

15-09-2025 14:40

Nicolas VAN VOOREN Nicolas VAN VOOREN

Hello.I'm searching for a digital copy of the seco

14-09-2025 22:16

Philippe PELLICIER

Apothécies petites jusquà 3 mm, oranges, avec de

13-09-2025 14:01

Thomas Flammer

dark brown apothecia, splitIKI-Spores biguttulate

10-09-2025 17:18

Blasco Rafael Blasco Rafael

Hola, encontre este estiercol de vaca estos apotec

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Asco on soil
Robin Isaksson, 19-10-2020 22:04
Robin IsakssonFound on soil sling with mosses.

Asci lugols negative. Spores 11-14x5-7 um
Have no clue where to look.
Somehe knows it?

Regards
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Hans-Otto Baral, 19-10-2020 22:17
Hans-Otto Baral
Re : Asco on soil
Uff, could you send more pics of the spores? And make a test: do the drops in the spores disappear in KOH?

This sounds like a Bryorbilia (Orbiliomycetes), a genus for which only one collection is known so far!

Is this a very sparse sample?
Robin Isaksson, 19-10-2020 22:54
Robin Isaksson
Re : Asco on soil
Hi!

Here come pictures of the spores. Sorry about quality.

It seems like the drops remains in koh 10%.
Last picture in koh.
Tested koh+lugols too now and it is no reaction in asci.

There is around 10 apotecia in the collection, i can collect moore because it was plenty of it in the slope.
But it has been cold now 3 days with minus degrees.


Regards
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Hans-Otto Baral, 20-10-2020 08:43
Hans-Otto Baral
Re : Asco on soil
Ah, this would be good if you collect further apos. Please dry them carefully and send them to someone who does sequencing, perhaps Guy Marson. From where is the sample, is the geology acidic?
Robin Isaksson, 20-10-2020 20:42
Robin Isaksson
Re : Asco on soil
Hi,

ok i will collect more on friday.
Its from Sweden, provins Småland. was found on a pile of earth but I have a hard time believing that there would be lime in the ground because everything around was silicate rocks sticking up. we have very little land with lime where I live. so yes its acidic.

I am happy to send a collection to someone who wants it for sequencing. do you have an email to some?


all the best
Hans-Otto Baral, 20-10-2020 20:55
Hans-Otto Baral
Re : Asco on soil
O.k., thanks! The only Bryorbilia collection so far known was also on silicate but from Asturias.

I hope I can tell you soon an address.
Hans-Otto Baral, 21-10-2020 11:00
Hans-Otto Baral
Re : Asco on soil
Yes, Guy would like to sequence it. Please let me know your e-mail then I send you his address.

Guy says: please not as registered mail - and the material should be dried beforehand on a "small heat source" (e.g. near the transformer of an electrical appliance or on a radiator).
Robin Isaksson, 21-10-2020 21:07
Robin Isaksson
Re : Asco on soil
hi,

Great!

robinisaksson69(at)gmail.com 

Hans-Otto Baral, 10-11-2020 20:58
Hans-Otto Baral
Re : Asco on soil
For all a good news: sequencing was successful and this species can be assigned to Bryorbilia arenicola, though with 8 nt deviation in the ITS1 but none in ITS2 and LSU D1-D3 (ITS distance 1-1.2%). Unnamed environmental strains of Bryorbilia spp. and other genera are very difficult to align in the ITS and differ by min. 8% in the LSU.
Robin Isaksson, 10-11-2020 22:25
Robin Isaksson
Re : Asco on soil
Thank you for the help with this one!


Regards,

Robin