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13-06-2025 09:41

Josep Torres Josep Torres

Hello.A cerebriform ascomycete sprouting scattered

13-06-2025 16:34

Andgelo Mombert Andgelo Mombert

Bonjour,Un petit discomycète qui me résiste. Il

24-07-2025 19:02

Castillo Joseba Castillo Joseba

De esta mañana en tronco caido de Haya A ver si

21-07-2025 19:22

Ethan Crenson

Hello all,  Here is an Orbilia found by a friend

14-07-2025 11:20

Michel Hairaud Michel Hairaud

Bonjour, Voici une espèce de  (?) Hyaloscyphace

18-07-2025 23:03

Josep Torres Josep Torres

Hello.Fruitings between 51 and 130 microns in tota

17-07-2025 11:55

Castillo Joseba Castillo Joseba

De ayer en bosque de hayas y abetos, en tieraEjemp

16-07-2025 17:34

Bernard Declercq Bernard Declercq

Hello,I have trouble distinguishing above mention

15-07-2025 13:27

Angel Pintos Angel Pintos

Hello, does anyone have access to the following ar

16-01-2023 21:31

Riet van Oosten Riet van Oosten

Hello, Nearby the find of Calycina claroflava on

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Tiny yellow apothecia on Juncus trifidus in alpine habitat
Edvin Johannesen, 17-08-2020 14:56
Edvin Johannesen
Hi!

This tiny (1-1.5 mm diam.) inoperculate discomycete was growing on old culms of Juncus trifidus ("probably", according to the collector who is botanically inclined) 1230 m a.s.l., which is alpine zone above the timberline in Central Norway. The habitat is described as bog/mire.

Spores are initially non-septate, but eventually develop one septum. They measure (16-)17-18(-20) x 5-6 microns and contain several guttules of varying size (live spores in water).  Paraphyses are guttulate and contain yellow pigment.  The apothecia are soft and do not appear to be gelatinized. I have not tested IKI reaction, doubting that it would be of major significance.

I was (mis?)lead in the direction of Calycina scolochloae, but I think that is a wrong track.

Any suggestion?

Thanks!

Edvin
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Hans-Otto Baral, 17-08-2020 16:00
Hans-Otto Baral
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
This actually requires the iodine reaction (Lugol). Shape of the ring in a turgorless ascus is necessary to see, also the colour. Croziers present?
Edvin Johannesen, 17-08-2020 16:11
Edvin Johannesen
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
Thanks, Zotto. I guess a turgorless ascus is one that has discharged the spores? I will try also to show the ascus basis. KOH, washing and then Lugol (or Baral's - I have both)?
Edvin Johannesen, 18-08-2020 12:47
Edvin Johannesen
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
This is after KOH treatment, twice washing with water, and then Lugol. Immature asci are stained orange, less so upon maturation, it seems. I am uncertain about ascus apparatus staining - possibly a very thin ring (blue).
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Edvin Johannesen, 18-08-2020 12:50
Edvin Johannesen
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
This is with Lugol only.
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Edvin Johannesen, 18-08-2020 12:52
Edvin Johannesen
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
And I believe there are croziers.
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Hans-Otto Baral, 18-08-2020 22:04
Hans-Otto Baral
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
So it is clearly with croziers, and the asci are clearly inamyloid in my estimation. I have no clear idea, the only being Phaeohelotium monticola on an unusual substrate, with unusually long apothecial stipes.
Edvin Johannesen, 19-08-2020 10:50
Edvin Johannesen
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
Thanks, Zotto. I suspect I might have the same as Maren Kamke found back in 2015: >



Although the spores are slightly larger in our collection. Not sure in what medium Karen measured, though.
Hans-Otto Baral, 19-08-2020 11:01
Hans-Otto Baral
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
Indeed! Maren surely also used water. Your spores actually are a bit too large for the species.
Edvin Johannesen, 19-08-2020 11:15
Edvin Johannesen
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
Thanks!
Edvin Johannesen, 20-08-2020 12:56
Edvin Johannesen
Re : Tiny yellow apothecia on Juncus trifidus in alpine habitat
I have now re-measured the spores and updated the numbers in the original post. They measure mostly 17-18 x 5-6 microns in water. Rarely shorter (16), and never narrower. A few spores 19-20 long.  Still slightly larger than Maren Kamkes collection, but I feel more comfortable now, that they are the same.