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25-01-2026 23:23

Tomaz Vucko Tomaz Vucko

Hello! I found this species that resembles Delitsc

06-02-2026 01:14

Tomaz Vucko Tomaz Vucko

Hello!How would you name this species? Most perith

04-08-2014 17:35

Leandro Sánchez Leandro Sánchez

Sur branche de feuillus, jusqu'à 22 mm diamètre,

05-02-2026 15:07

Vasileios Kaounas Vasileios Kaounas

Found on a fallen needle of Pinus halepensis, diam

05-02-2026 06:43

Stefan Blaser

Hello everybody, Any help on this one would be mu

18-08-2025 15:07

Lothar Krieglsteiner Lothar Krieglsteiner

.. 20.7.25, in subarctic habital. The liverwort i

02-02-2026 21:46

Margot en Geert Vullings

On a barkless poplar branch, we found hairy discs

02-02-2026 14:55

Andgelo Mombert Andgelo Mombert

Bonjour,Sur thalle de Lobaria pulmonaria.Conidiome

02-02-2026 14:33

Andgelo Mombert Andgelo Mombert

Bonjour,Sur le thalle de Peltigera praetextata, ne

31-01-2026 10:22

Michel Hairaud Michel Hairaud

Bonjour, Cette hypocreale parasite en nombre les

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Preservation of Ascobolaceae members
Suhaib Firdous Yatoo, 18-03-2020 19:03
Suhaib  Firdous YatooWhat is the best way to preserve Ascobolaceae members, mostly the smaller ones that are less than 1mm and how to preserve them for herbarium purposes?
How to rehydrate if I want to work on them again?
(as KOH dissolves the episporium).
Hans-Otto Baral, 18-03-2020 19:05
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
The by far very best way is to study the in the fresh state. Everything else leads to minor results and includes artifacts.
Suhaib Firdous Yatoo, 19-03-2020 10:49
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
How to preserve them for herbarium?
And can we preserve the smaller ones as slides mounted in Hoyer?
Hans-Otto Baral, 19-03-2020 10:55
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
I can only recomend you to read my paper from 1992 (Mycotaxon).

Preservation in Ascobolus is not different from any other ascomycete. Drying and placing in a tight plastic bag. Hoyer's liquid I remember a bit, it is killing the cells and perhaps also dissolves structures.
Suhaib Firdous Yatoo, 19-03-2020 11:07
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
Yes, and cells seem to swell a bit.
Thank you very very much sir.