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me mandas el material seco de Galicia (España) re
Albotricha or Lasiobelonium?
Edvin Johannesen,
14-04-2019 14:57
I am struggling a bit to identify this one. First I arrived at Albotricha laetior, but the paraphyses are not broadly lanceolate (they are narrowly laceolate to cylindrical). Then I probed Lasiobelonium variegatum, but the hairs don't quite fit, I think. Spores are in the range 10x3 my and become 1-septate at maturity. Hairs thick-walled and brown, except for the pointed tips. Some hairs have granules in water, and some have a rounded tip. Ascus pore reacts purplish brown in Baral's Lösung. Growing on bark of Populus tremula. Photos in water diluted Cotton Blue.
Edvin Johannesen,
14-04-2019 14:59
Re : Albotricha or Lasiobelonium?
In water (phase contrast (sorry)). Incrusted hairs and paraphyses.
Edvin Johannesen,
14-04-2019 14:59
Re : Albotricha or Lasiobelonium?
Ascus tip in Baral's.
Edvin Johannesen,
16-04-2019 15:18
Re : Albotricha or Lasiobelonium?
I have added better photos of fresh apothecia. I hope someone has an opinion.
Hans-Otto Baral,
16-04-2019 15:35
Re : Albotricha or Lasiobelonium?
This requires to know the oil content of the spores. Any photos in water? Important also whether the spores are ejected when 1-septate or non-septate. The large hyaline regions look like oil bodies (you can also mount in KOH if nothing is alive). Perhaps L. lonicerae?
Edvin Johannesen,
16-04-2019 15:53
Re : Albotricha or Lasiobelonium?
Thanks, Zotto. The spores are septate while still inside the asci. I will image spores in water to check the oil drops.
Hans-Otto Baral,
16-04-2019 16:15
Re : Albotricha or Lasiobelonium?
I saw this but this says little. As I am sermonizing since decades, spores may get overmature inside dead asci. You must view living asci, otherwise you cannot be sure.
Edvin Johannesen,
16-04-2019 16:22
Re : Albotricha or Lasiobelonium?
Ok. I am not sure how to decide whether asci are alive or not, but here are som pictures in water. Septa are formed within the asci. So are large oil drops, but it may seem like they are lost when spores are ejected. Not sure if this helps. Obviously I am looking at dried material (collected in March). By the way, the hairs look more obtuse now, pale brown, septate and apically somewhat incrusted.
Hans-Otto Baral,
16-04-2019 16:49
Re : Albotricha or Lasiobelonium?
This is the first prerequisite when studying ascos. Look on the frontpage of my old homepage:
http://www.gbif-mycology.de/HostedSites/Baral/
Yes, big oil drops. This excludes L. variegatum which in addition has undulating hairs on the underside.
The frequency of septate spores in the asci and the good-looking sample suggests L. lonicerae.
http://www.gbif-mycology.de/HostedSites/Baral/
Yes, big oil drops. This excludes L. variegatum which in addition has undulating hairs on the underside.
The frequency of septate spores in the asci and the good-looking sample suggests L. lonicerae.
Edvin Johannesen,
16-04-2019 17:05
Re : Albotricha or Lasiobelonium?
Thanks again. I once had the instructive front page of yours, but somehow it got lost. I know the importance of live vs. dead asci and spores, but just didn't have a good information source - until now :-)
I checked L. lonicerae in the meantime and it makes perfect sense. I also excluded L. variagatum in the first round due to a comment from you in a related topic here about the undulating hairs below.
Best regards and a happy Easter to all.