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22-05-2025 08:55

Josep Torres Josep Torres

Hello.A Pezizal sprouting scattered in small group

21-05-2025 17:28

éric ROMERO éric ROMERO

Bonjour Tous, Avec la bonne fiche cette fois (mer

19-05-2025 09:42

Josep Torres Josep Torres

Hello.An ascomycete photographed on April 20, spro

21-05-2025 08:31

Josep Torres Josep Torres

Reddish-brown, grain-like ascomata, measuring 0.7â

20-05-2025 09:37

Josep Torres Josep Torres

Hello, an anamorph photographed last April 20th, s

20-05-2025 22:15

Francois Guay Francois Guay

I found this ascomycete at the base of a dead fern

20-05-2025 21:36

Francois Guay Francois Guay

I found this Mollisia sp. on a submerged herbaceou

20-05-2025 10:31

Castillo Joseba Castillo Joseba

me mandan elmaterial seco de Galicia, (España) 

16-05-2025 05:47

Francois Guay Francois Guay

I found this super tiny hyaline asco on fir needle

11-05-2025 10:22

Karl Soler Kinnerbäck

Found on moist Betula wood dipped in freshwater st

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Maybe Mollisia lividofusca?
François Bartholomeeusen, 08-03-2016 08:17
Hi everyone,

Mollisia found on decorticated branch covered with algae. 

FRB: diameter 1.5 mm; gregarious in small clusters, hymenium light grey/blue, edge and bottom dark black brown,

Microscopy (all dimensions in water): of fresh material

Asci: cylindrical, hyaline, pointy top, amyloid; with croziers, 67-73 x 7.35-7.61 µm.
Spore: variable: cylindrical, amygdaloid to oblong with one septum; 7.58-12.08 x 2.62-3,68 µm; Q= 2,92 
Paraphyse: with swollen top and refractive vacuole
Texture:
- subhymenium texture intricata light brown hyphae
- ectal excipulum:dark brown, texture globulosa
- medullary excipulum: light brown, globulosa-angularis.

What is your opinion, can it be Mollisia lividofusca?

Thanks in advance!

Greetings,

François Bartholomeeusen 

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Andreas Gminder, 08-03-2016 08:24
Andreas Gminder
Re : Maybe Mollisia lividofusca?

Hello,


I'm not too certain. The colour of the subhymenium is not to see in your foto, and I'm not sure wether you may be mixed up the subhymenium and the excipulum? The loosely arranged medulla below the subhymenium would be a good hint towards M. lividofusca, but the macroscopy is unusual pale. In this wet stage M. lividofusca would either show distinct brownish tinges in the hymenium or be dark blueish-grey. Also the septate spores are very unusual for M. lividofusca and last the spore width also in unusual broad.


What cocnerns the spores that may be an abnormal developement due to the winter temperatures may be?
Did you check the KOH-reation (it is most likely negative, but it's always worth a try ...)
And are you really certain about the colour of the subhymenial hyphae?


best regards,
Andreas

François Bartholomeeusen, 08-03-2016 16:13
Re : Maybe Mollisia lividofusca?
Hi Andreas,

Thanks for your fast answer. You are right, due to the low temperature in the morning last week, the apothecia suffered probably from frostbite. I add a picture of spores with a more regular pattern which I measured again: 8,68-11,25 x 2,43-3 µm; Q= 4.05.
I also add two pictures of the frb, one by daylight and one through the binocular, and a picture of a new preparation. Would you be so kind te check the color of the hymenium and of the subhymenial hyphae. I am sorry to say that I am out of KOH.


Can this information shed some light on a determination?

Greetings,

François 

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Andreas Gminder, 08-03-2016 16:17
Andreas Gminder
Re : Maybe Mollisia lividofusca?

Hello,


the daylight foto looks very much like M. lividofusca.


The other fotos I can not see too clear. A pitty that you don't have KOH, because if you have a thin cut through the apothecium in KOH, you can see the coloured subhymenium far better because all those refractive vacuoles are dissolved and don't mask the true colour of the hyphae.


best regards,
Andreas

François Bartholomeeusen, 08-03-2016 16:38
Re : Maybe Mollisia lividofusca?
Thank you very much Andreas, I have to buy some KOH and follow your advice!

Until the next time,

François
François Bartholomeeusen, 09-03-2016 21:02
Re : Maybe Mollisia lividofusca?
Hello Andreas,

I have used KOH but not without problems! Cutting the apothecium was not easy, I couldn't get a nice sharp picture ... and I only saw a green discoloration. For me everything is still very unclear, what do you think of it?

Greetings,

François
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