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Help with this one much appreciated, on rotting Fa
Marcelleina aff benkertii 211214 47
Miguel Ángel Ribes,
20-02-2015 00:25
Good night againThis dark-violet, 4-5 mm Marcelleina was growing on clayey soil, with some mosses in a mixed forest of Pinus canariensis and "fayal-brezal" (Myrica faya, Erica scoparia, Laurus novocanariensis, Ilex canariensis, etc.) at Tenerife (Canary Islands) at 1.300 masl.
Ectal excipulum with textura angularis, more globulosa towards the margin. Base plenty of long "hairs" or anchor hyphae, hyaline, septate and thin.
Medullar excipulum with textura cylindrical perpendicular to the hymenium to intricata.
Subhymenium with textura globulosa.
Asci 8-spored, inamyloid and with croziers.
Spores globose, hyaline, with a very big and mostly centered guttule, (9.4) 9.7 - 10.5 (10.9) x (9.3) 9.6 - 10.3 (10.6) µm; Q = (1.0) 1.01 - 1.0; N = 56; Me = 10.1 x 9.9 µm ; Qe = 1.0.
Paraphysis straight, septate, slightly broad at apex and with violet pigment.
Thank you for your help.
Miguel Á. Ribes
Hans-Otto Baral,
20-02-2015 06:34
Re : Marcelleina aff benkertii 211214 47
Dear Miguel-Angel
I canot help but I would like to see the living paraphyses with their violet contents, also the turgescent asci.
Zotto
I canot help but I would like to see the living paraphyses with their violet contents, also the turgescent asci.
Zotto
Miguel Ángel Ribes,
20-02-2015 08:56
Re : Marcelleina aff benkertii 211214 47
Here they are, Zotto.
Nicolas VAN VOOREN,
20-02-2015 10:09
Re : Marcelleina aff benkertii 211214 47
Hi.
Did you test the reaction of a small piece in HCl? It could be a good test for comparison with Smardaea species.
Did you test the reaction of a small piece in HCl? It could be a good test for comparison with Smardaea species.
Miguel Ángel Ribes,
20-02-2015 11:31
Re : Marcelleina aff benkertii 211214 47
No, sorry, Nicolas, but I don't know that reaction, could you explain it?, please.
Anyway, the only Smardea with globose spores in the papers I have is S. planchonis, but I see another guttules pattern in this species.
Thank you.
Anyway, the only Smardea with globose spores in the papers I have is S. planchonis, but I see another guttules pattern in this species.
Thank you.
Nicolas VAN VOOREN,
20-02-2015 11:41
Re : Marcelleina aff benkertii 211214 47
HCl dissolves pigments and this produces a red reaction of the flesh. I'm not sure that pigments present in Marcelleina species give the same reaction. This is just to test ;)
Miguel Ángel Ribes,
20-02-2015 12:31
Re : Marcelleina aff benkertii 211214 47
Only with fresh apos? Macro-reaction, without micro?
Thanks.
Thanks.
Miguel Ángel Ribes,
24-02-2015 17:24
Re : Marcelleina aff benkertii 211214 47
Sorry for the delay ... I hadn't HCl.
Completley negative reaction with HCl 35%, nor macro, nor micro.
Any other idea?
Thank you
Completley negative reaction with HCl 35%, nor macro, nor micro.
Any other idea?
Thank you
Nicolas VAN VOOREN,
25-02-2015 15:06
Re : Marcelleina aff benkertii 211214 47
Thanks for the test. So Smardaea is excluded in my opinion, and the hypothesis of M. benkertii seems good.
Another reference:
Di Meo A. & Lalli G. 1993. Marcelleina benkertii, un fungo a coppa a spore sferiche nuovo per l'Italia. Micologia e Vegetazione Mediterranea, 8 (1) : 3-8.
Another reference:
Di Meo A. & Lalli G. 1993. Marcelleina benkertii, un fungo a coppa a spore sferiche nuovo per l'Italia. Micologia e Vegetazione Mediterranea, 8 (1) : 3-8.
Miguel Ángel Ribes,
25-02-2015 21:04
Re : Marcelleina aff benkertii 211214 47
Your wellcome, Nicolas
I can't get the paper in the internet and I don't know anyone who has it, anyone have it?
Di Meo A. & Lalli G. 1993. Marcelleina benkertii, un fungo a coppa a spore sferiche nuovo per l'Italia. Micologia e Vegetazione Mediterranea, 8 (1) : 3-8.
Thank you.
I can't get the paper in the internet and I don't know anyone who has it, anyone have it?
Di Meo A. & Lalli G. 1993. Marcelleina benkertii, un fungo a coppa a spore sferiche nuovo per l'Italia. Micologia e Vegetazione Mediterranea, 8 (1) : 3-8.
Thank you.
Miguel Ángel Ribes,
11-03-2015 20:05
Re : Marcelleina aff benkertii 211214 47
Hi again friends
Finally I got the paper and re-study this species. Reading that paper and Moravec 1987 I had doubts with genus Greletia, and I have extract this differences between both:
Pigment
* Greletia: purplish in the asci, ascospores, paraphyses and excipulum. Soluble in KOH.
* Marcelleina: violet-purplish only in paraphyses. Not soluble in KOH.
Stipe
* Greletia: substipitate.
* Marcelleina: non stipitate.
Medullary excipulum
* Greletia: composed of a loose textura intricata well differentiated from the outer layer which consists of a textura globulosa.
* Marcelleina: composed of a textura intricata to textura prismatica or textura globulosa-angularis, the hyphae being densely arranged and inflated.
Ectal extipulum
* Greletia: textura globulosa with wall cells pigmented in purplish.
* Marcelleina: textura globulosa with wall cells pigmented in light brown.
Spores
* Greletia: contain purplish pigment and are sculptured (only visible with SEM).
* Marcelleina: hyalines and sculptured, with the exception of M. benkertii.
With this table I think there is no doubt this species belongs to Marcelleina genus, and the only possible species is M. benkertii, but I have a little doubt about the interpretation on the medullary excipulum, because I see a textura cylindrical perpendicular to the hymenium to intricata, more near to Greletia description than Marcelleina. In the attached pictures it is visible.
Thank you for your opinion.
Finally I got the paper and re-study this species. Reading that paper and Moravec 1987 I had doubts with genus Greletia, and I have extract this differences between both:
Pigment
* Greletia: purplish in the asci, ascospores, paraphyses and excipulum. Soluble in KOH.
* Marcelleina: violet-purplish only in paraphyses. Not soluble in KOH.
Stipe
* Greletia: substipitate.
* Marcelleina: non stipitate.
Medullary excipulum
* Greletia: composed of a loose textura intricata well differentiated from the outer layer which consists of a textura globulosa.
* Marcelleina: composed of a textura intricata to textura prismatica or textura globulosa-angularis, the hyphae being densely arranged and inflated.
Ectal extipulum
* Greletia: textura globulosa with wall cells pigmented in purplish.
* Marcelleina: textura globulosa with wall cells pigmented in light brown.
Spores
* Greletia: contain purplish pigment and are sculptured (only visible with SEM).
* Marcelleina: hyalines and sculptured, with the exception of M. benkertii.
With this table I think there is no doubt this species belongs to Marcelleina genus, and the only possible species is M. benkertii, but I have a little doubt about the interpretation on the medullary excipulum, because I see a textura cylindrical perpendicular to the hymenium to intricata, more near to Greletia description than Marcelleina. In the attached pictures it is visible.
Thank you for your opinion.
Hans-Otto Baral,
11-03-2015 20:28
Re : Marcelleina aff benkertii 211214 47
My info is that Greletia is a synonym of Smardaea, but I really am not familiar with these fungi :-(
Zotto
Zotto
Miguel Ángel Ribes,
11-03-2015 20:44
Re : Marcelleina aff benkertii 211214 47
Yes, I think so too, perphaps I must wrote Smardaea instead Greletia, sorry, now I see in MycoBank and IndexFungorum than the current name is Smardaea.
Thank you.
Thank you.