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Didymella applanata??
hannie wijers,
26-03-2013 12:54
I hope anyone can help me with my "problem"
On the branches of a blackberry bush I found this little black pimples. Under the microscope, I got the next results. Could this be a Didymella applanata? The spores were 12-16x6 uM at large magnification of 1000x
Thanks
Hannie
Björn Wergen,
26-03-2013 13:09
Re : Didymella applanata??
Hi,
no, this is a member of Amphisphaeria, please check ascus apical structures for reaction with Lugol.
regards,
björn
no, this is a member of Amphisphaeria, please check ascus apical structures for reaction with Lugol.
regards,
björn
hannie wijers,
26-03-2013 14:53
Björn Wergen,
26-03-2013 19:49
Re : Didymella applanata??
Hi,
correct, I meant this. But I cannot see wether it is positive or negative. There are several (3-4) species of Amphisphaeria with negative Lugol/Melzer reaction, like Amphisphaeria vibratilis or A. paedida.
Did the spore colour chance from brown to green in melzer?
regards,
björn
correct, I meant this. But I cannot see wether it is positive or negative. There are several (3-4) species of Amphisphaeria with negative Lugol/Melzer reaction, like Amphisphaeria vibratilis or A. paedida.
Did the spore colour chance from brown to green in melzer?
regards,
björn
Jaklitsch Walter,
26-03-2013 22:57
Re : Didymella applanata??
I would suggest Didymosphaeria oblitescens, if the spores have a foveate ornamentation (not clear from the images), or D. futilis.
Regards, Walter
Regards, Walter
Alain GARDIENNET,
27-03-2013 07:31
Re : Didymella applanata??
I also thought at the genus Didymosphaeria (oblitesecens with its foveolate ascospores is fairly common on Rubus), I agree with Walter.
There is a key in Studies Mycology 37.
We can guess ornementation on the photo of asci. How exactly is it ?
Alain
There is a key in Studies Mycology 37.
We can guess ornementation on the photo of asci. How exactly is it ?
Alain
hannie wijers,
27-03-2013 12:13
Re : Didymella applanata??
Good morning,
I downloaded the key of Didymosdphaeria. The spores didn't colour green an as Ik looked again this mornig it's still J- and the spores have a foveate ornamentation.
Looking at the key I suggest that it is D. oblitescens? I think 1b, 2a, 3a =D.oblitescens.
Ascospores less than three times as long as wide.
I hope you correct me if I'm wrong.
I thank all of you for the help, in this way I still learn a lot.
regards
Hannie
Björn Wergen,
27-03-2013 13:09
Re : Didymella applanata??
I still not agree with Didymosphaeria oblitescens, because I remember an own finding of exatly this species on Rubus fruticosus agg. in 2011 (first 2 pictures).
The last 2 pics show the real Didymosphaeria oblitescens growing on dead Cornus alba stems, which has been determined by several specialists in this forum.
I feel that there is something wrong...
regards,
björn
The last 2 pics show the real Didymosphaeria oblitescens growing on dead Cornus alba stems, which has been determined by several specialists in this forum.
I feel that there is something wrong...
regards,
björn
hannie wijers,
27-03-2013 14:23
Re : Didymella applanata??
Hello björn,
It's ok to me if you think I'm not right, in this way I learn more of it. When I looked at your pictures the first two looks more like my pictures. Maybe I was wrong, the spores didn't have foveate ornamentation but warty spores. I don't have an expensive microscoop.
regards Hannie
It's ok to me if you think I'm not right, in this way I learn more of it. When I looked at your pictures the first two looks more like my pictures. Maybe I was wrong, the spores didn't have foveate ornamentation but warty spores. I don't have an expensive microscoop.
regards Hannie
Yannick Mourgues,
27-03-2013 19:32
Re : Didymella applanata??
Bonjour.
Qui peut me traduire le mot "foveate ornamentation?" ?
Merci.
Yannick
Qui peut me traduire le mot "foveate ornamentation?" ?
Merci.
Yannick
hannie wijers,
27-03-2013 19:36
Re : Didymella applanata??
En ce qui concerne ce que j'ai compris de la verrue littérature, mais pas d'épines.Peut-être que je me trompe
Hannie
Hannie
Alain GARDIENNET,
27-03-2013 20:39
Re : Didymella applanata??
Salut Y,
Les fovéoles sont de petites dépressions (concavités) alors que les verrues sont
des convexités.
Alain
Les fovéoles sont de petites dépressions (concavités) alors que les verrues sont
des convexités.
Alain
Jaklitsch Walter,
27-03-2013 21:04
Re : Didymella applanata??
exactly; foveolate is also just used for "warted below the perispore", i.e. the warts do not protrude
Alain GARDIENNET,
28-03-2013 21:07
Re : Didymella applanata??
In conclusion, we can say that the records of Rubus (from Hannie and Björn) should be oblitescens and the one on Cornus (Björn's one) should be more according with futilis.
A positive discussion.
Alain
A positive discussion.
Alain
Björn Wergen,
28-03-2013 21:26
Re : Didymella applanata??
Yes that's it :)
hannie wijers,
29-03-2013 09:29
Re : Didymella applanata??
Thank you Björn and Alain. I didn't expect that it caused so much "trouble" for the right name. Now I could tell my companions the rigtht name. Thanks again.
Hannie
Hannie
Hiran Hiran Anjana Ariyawansa,
21-04-2013 08:44
Re : Didymella applanata??
Hii
I also belive this sp. is belong to Didymosphaeria
Eeventhough this genus is well established the familial placement of the genera is uncertain we need fresh collections to sort out this problem
Could please loan me the material to check if so it would be greatful where i try to do some molecular work on it
Thank you and look forward to hearing from you
HA
I also belive this sp. is belong to Didymosphaeria
Eeventhough this genus is well established the familial placement of the genera is uncertain we need fresh collections to sort out this problem
Could please loan me the material to check if so it would be greatful where i try to do some molecular work on it
Thank you and look forward to hearing from you
HA
hannie wijers,
21-04-2013 12:04
Re : Didymella applanata??
HA
If I read it correct you wanted some material send? I found it on Rubus. I still have the twiggs. If you want something of it if to examen I have to take a look if I can find it. But this will be in a few days.
Regards Hannie
If I read it correct you wanted some material send? I found it on Rubus. I still have the twiggs. If you want something of it if to examen I have to take a look if I can find it. But this will be in a few days.
Regards Hannie
Hiran Hiran Anjana Ariyawansa,
21-04-2013 12:48
Re : Didymella applanata??
Dear Hannie
Yes please can you send me the material
So i can try work on the molecular on the species
ariyawansa44@gmail.com is my e mail you can contact me via on that
hope we can have intersting finding on this
Thank you
HA
Yes please can you send me the material
So i can try work on the molecular on the species
ariyawansa44@gmail.com is my e mail you can contact me via on that
hope we can have intersting finding on this
Thank you
HA