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Does anyone have the following paperMycocaliciacea

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Fusarium on Hibiscus
Martin Bemmann, 06-05-2012 23:34
Martin BemmannIn my garden two shrubs of Hibiscus (15 to 20 years old) did not survive the winter season. Today I observed lots of orange dots down to the roots that turned out to be conidiocarps of a Fusarium species. The last service I try to give to these beloved flowers  is to give their hangman a name. ;-)
I attache some pictures, but the host will be certainly more helpful for determination, for those who know....

Typical conidia show 3 septa but 0-4 septa were observed as well.

Best regards

Martin
  • message #18496
  • message #18496
  • message #18496
  • message #18496
Chris Yeates, 07-05-2012 01:54
Chris Yeates
Re : Fusarium on Hibiscus
hello Martin

others with greater knowledge may contradict me, but my experience is that Fusarium is a 'culture or sequence' genus - easy to recognise at the generic level but impossible to name on macroscopic and microscopic information of field characters alone, unfortunately

Chris
Hans-Otto Baral, 07-05-2012 07:43
Hans-Otto Baral
Re : Fusarium on Hibiscus
Hi Martin & Chris

I made the same experience :-(
http://www.ascofrance.fr/search_forum/11579?

But you have the opportunity to await the teleomorph if any exists. Or did you remove the shrubs?

We have here also Hibiscus shrubs in gardens which look rather ill. I will have a look.

Are these Fusarium species really polyphagous? Or does the host help in the identification?

Zotto


Christian Lechat, 07-05-2012 10:09
Christian Lechat
Re : Fusarium on Hibiscus
Hi Martin,
I would like to study and sequence your Fusarium, could you please to send me a part of your collection?

a lot of thanks
Christian
Martin Bemmann, 07-05-2012 10:23
Martin Bemmann
Re : Fusarium on Hibiscus
Hi Christian,

I have tons of it...

I will send you some by tomorrow.

Best regards,

Martin
Christian Lechat, 19-11-2012 21:33
Christian Lechat
Re : Fusarium on Hibiscus
Dear all,
here are molecular data of Fusarium on Hibiscus, no doubt, that is Gibberella pulicaris.

All my best

Chirstian

  • message #20460
Martin Bemmann, 20-11-2012 23:41
Martin Bemmann
Re : Fusarium on Hibiscus
Dear Christian,

thank you very much for your efforts!
I have but a problem with your result. When I run a BLAST at GenBank with your sequence CLL12012_ITS4 the best match showing up is  F. merismoides (not even fitting macroscopically to my find). 
Is this due to the fact you choose ITS4 (no entry for Fusarium at GenBank features it) I cant't comprehend your result? I have to learn very much in this field!

curiously,

Martin

?

Martin Bemmann, 21-11-2012 19:56
Martin Bemmann
Re : Fusarium on Hibiscus
Dear Christian,

attached an alternative tree. I added the well matching F. merismoides and the common F. oxysporum. The outgroup Microdochium nivale I choose following Watanabe et al. 2011.

Best regards

Martin
  • message #20487
Christian Lechat, 22-11-2012 14:36
Christian Lechat
Re : Fusarium on Hibiscus
Sorry dear Martin,
I made a mistake about sequence, the one I have posted is not from your specimen but it is a Dialonectriaa.
I attach the correct sequence.

excuse me,

Regards,
Christian
Martin Bemmann, 22-11-2012 21:19
Martin Bemmann
Re : Fusarium on Hibiscus
Thank you Christian,

that explains a lot! So the name of my Hibiscus killer will be Fusarium sambucinum... ;-)
No, I learned meanwhile that last winter many shrubs died due to frost and those Fusarium fungi just entered the dead or dying plants.

Regards

Martin