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03-09-2025 12:44

Enrique Rubio Enrique Rubio

Hi to somebody.I would like to know your opinion o

02-09-2025 10:28

Castillo Joseba Castillo Joseba

De ayer en madera muy podrida de haya (Fagus)A ver

31-08-2025 19:41

Enrique Rubio Enrique Rubio

Hi to someone.I need to download this issue of Sve

02-09-2025 11:34

Thomas Læssøe

https://svampe.databasen.org/observations/10527903

31-08-2025 17:32

Michel Hairaud Michel Hairaud

Bonjour, Pas d'identité trouvée pour cette réc

01-09-2025 08:42

François Corhay

Un ami, conservateur d'une RN en Belgique, m'a adr

31-08-2025 14:34

Thomas Flammer

I have found a Rutstroemia on abt. 2000 m on Alnus

29-08-2025 19:04

Thomas Flammer

Spores 21.2 - 26.2 x 8.3 - 11.3 µm - Q: 2.20 - 2.

30-08-2025 18:29

Joaquin Martin

Hi,I found this undetermined ascomycete on broadle

28-08-2025 17:24

Thomas Flammer

I know, that this is not the real topic of this fo

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Wall or Sheath
Romina Sánchez, 03-05-2010 18:10
Hello everyone, I whant to ask you if there is any technique or dye reagent that I can use to distinguish between a hyaline-wall and a sheath from ascospores.

Regards
Romina
Christian Lechat, 03-05-2010 18:24
Christian Lechat
Re:Wall or Sheath
You would have to use Black ink to see the sheath.
Christian
Romina Sánchez, 03-05-2010 19:00
Re:Wall or Sheath
Thank you Chirstian, I appreciate your quick reply!
Romina
Hans-Otto Baral, 09-05-2010 00:13
Hans-Otto Baral
Re:Wall or Sheath
I also recommend to use Cresyl blue (aqueous). Many gel sheaths stain bright lilac herein, but sometimes distinct sheaths are negative.

I also know the problem that the spore wall consists of several layers. F.ex. in Sarcoscypha the two layers are wall material, not gel sheath, but behave different: the outer layer is rigid and gets folded and separates when the inner elastic layer shrinks during turgor loss. The surface of the outer stains lilac in CRB which means a very thin gel on it.



Zotto
NC NC, 10-05-2010 17:56
Re:Wall or Sheath
thank you very much Zotto!
I have got ascospores with many layers and I whant to differentiate if the outter one is a sheat or not.
Your explanation is very useful, I will try with the cresyl blue.

Romina
Romina Sánchez, 10-05-2010 18:00
Re:Wall or Sheath
Thank you very much Zotto!
I have got ascospores with many layers and I want to differentiate if the outer one is a sheath or not.
Your explanation is very useful, I will try with the Cresyl Blue.

Warm regards
Romina
Jacques Fournier, 10-05-2010 19:11
Jacques Fournier
Re:Wall or Sheath
Hi Romina,
the sheaths of ascospores are of different chemical structure, which implies they react differently with stains. Aqueous nigrosin and chlorazol black also might be very useful, they sometimes also stain the appendages.
Cheers,
Jacques
Romina Sánchez, 10-05-2010 22:18
Re:Wall or Sheath
Thanks Jacques!
the people in this forum is wonderful!
And and you three (Christian, Zotto and Jacques), I saw that always respond the doubts of everybody. It's great that you share your knowledge.
Thank you again.

Romi