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Apothecia yellow, glassy-transparent, 80 - 120 ymS

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Rubus fruticosus disco
Chris Yeates, 14-11-2021 15:47
Chris Yeates
Bonjour tous

I have been doing some collecting on Rubus fruticosus (agg.) recently, and this is one of several Helotiales I have encountered.

Apothecia subsessile up to 300µm diameter, minutely downy, starting creamy and turning rusty-red, basal cells thick-walled, globose, running into elongated rather glassy hyphae, the terminal excipular cells rather thick-walled and ending in cells with refractive contents as can be seen in the images.

Asci 8-spored, in IKI deep blue, I could not see any croziers. Paraphyses often with extensive continuous to interrupted vacuolar contents. Ascospores (7.2)8.1-8.9 x 2.7-3.2µm, guttules as seen in the images. Enrique Rubio commented recently that many spores of Helotiales, when freshly ejected show a sort of "sheath"; in this instance a number of spores showed a sort of "halo" at one end - see image.

Associated with this apothecium were a number of two-celled hyaline bodies which appear fungal; these can be seen in the final photograph but also in #2.

I was wondering about Pezizellaceae? As ever, any comments would be appreciated.

Amitiés

Chris
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Hans-Otto Baral, 14-11-2021 17:03
Hans-Otto Baral
Re : Rubus fruticosus disco
Hi Chris

this reminded me of Calycellina separabilis, but the spores are much too short. Also, that species is strictly on R. idaeus as it seems, and it has croziers. With yours I am not sure with the absence of them.

You could test CRB if the spore sheath setectively stains bright lilac. Look at my photos Calycellina separabilis, HB 8584a-6, CRB

Did you change your E-mail address? I twice could not reach you.

Zotto
Chris Yeates, 14-11-2021 18:34
Chris Yeates
Re : Rubus fruticosus disco
Thanks Zotto

I agree, that species has a lot in common with my find. I will do some more work on it.

Spore length is clearly a problem, though in the protologue Karsten does say 8-15µm long. This site has a very large stand of Rubus idaeus immediately by where I found this and the two grow mixed together at the edge of it - indeed Orbilia rubrovacuolata seems to have "jumped" from fruticosus to idaeus here (I know I have mentioned this to you before). The substrate on one of the images in your C. separabilis folder looks rather like R. fruticosus.

My email address is csvy.myco@btinternet.com - I did change it, but quite a long while ago as I could only access the old one on my phone, and had to forward to the PC.

Best wishes, Chris
Hans-Otto Baral, 14-11-2021 22:12
Hans-Otto Baral
Re : Rubus fruticosus disco
I now saw I used a wrong address. My addressbock is not available on my present computer, no time to install ist.

If you mean C. separabilis 8.IX.2007, it is by Luc Baily. Indeed these spines look unlike R. idaeus, but Luc indicated that host anyway.