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Orbilia/Hyalorbilia on "pyrenomycete"
Edvin Johannesen,
24-05-2021 21:41

Hi!
This tiny (0.2-0.3 mm), orange one is growing on a stromatic sordariomycete (overgrown by something alike Echinosphaeria) on Salix caprea. I have no ID on the sordario yet. Southern Norway in humid ravine.
The Orbilia/Hyalorbilia has broadly ellipsoid spores with one distinct SB at each pole. Measure ca. 2.5-3 x 5 micr. Spores appear more subglobose in water and more ellipsoid with slightly pointed ends in CB (see photos). Dead asci (I think) do not look truncate. Paraphyses broadly cylindrical.
Suggestions based on this limited information?
Hans-Otto Baral,
24-05-2021 22:24

Re : Orbilia/Hyalorbilia on "pyrenomycete"
This is not orbiliaceous, perhaps a Hyaloscypha. Iodine reaction of asci is negative?
Edvin Johannesen,
24-05-2021 22:36

Re : Orbilia/Hyalorbilia on "pyrenomycete"
I will check IKI. (Why is is it not orbiliaceous?)
Thanks.
Hans-Otto Baral,
24-05-2021 23:01

Re : Orbilia/Hyalorbilia on "pyrenomycete"
The conical ascus apex would be very unusual, also there are no spore bodies I think.
Edvin Johannesen,
25-05-2021 16:28

Re : Orbilia/Hyalorbilia on "pyrenomycete"
You were right, of course, Zotto. The asci apices are hemiamyloid (brownish in Lugol, haven't checked KOH pretreatment). I initially thought there were two species growing side-by-side on the pyrenomycete, but now I realise that the Cistella-like whitish ones and the more reddish, smooth ones are the same. I attach a few photos og the paler, Cistella-like apothecia. I suspect I have the same as Enrique found back in 2014: http://ascofrance.fr/search_forum/27839
I think I have seen gelatinised hyphae. But the marginal "hairs" I have not spotted in the microscope. Has the Hyphodiscus "Cistella" been further evaluated?
Hans-Otto Baral,
25-05-2021 17:53

Re : Orbilia/Hyalorbilia on "pyrenomycete"
The limits to Hyphodiscus are not yet clear and there are no news about that.
Edvin Johannesen,
25-05-2021 18:06

Re : Orbilia/Hyalorbilia on "pyrenomycete"
OK, thanks!