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Eutypella (?) ... on Pinus.
Mirek Gryc, 29-08-2020 20:33
Hello all.
The fruiting bodies grew on a dead Pinus branch lying on the ground.
Spores: Me = 6.2 × 1.4 µm; Qe = 4.6
Reaction to iodine negative even after previous application of KOH.
Any suggestions are welcome.
best regards
Mirek
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Enrique Rubio, 30-08-2020 10:00
Enrique Rubio
Re : Eutypella (?) ... on Pinus.
Hi Mirek
I think you should look into the genus Valsa (Cytospora).
Mirek Gryc, 30-08-2020 17:13
Re : Eutypella (?) ... on Pinus.
Hello
Thank you so much to Enrique for the hint!
I suspected that it was not Eutypella, very short ascus for this genus.
The type of Valsa is completely unknown to me :(
I will definitely not be able to define the genre myself, so I am asking for some hints again.
I looked about it and found three species of the most common, occurring on pinus: V. pini; V. kunzei and V. friesii.
V. pini and V. frisii have larger spores.
But my spores and ascus dimensions match V. kunzei?
The width of ascus is about 4 µm so too narrow for V. pini and suitable for V. kunzei.
Could it be Valsa kunzei then ???
Best regards.
Mirek
Enrique Rubio, 30-08-2020 17:43
Enrique Rubio
Re : Eutypella (?) ... on Pinus.
Valsa is a very difficult genus that needs the knowledge of the asexual morph to try a correct determination.
You should have been able to see a conspicuous apical refractive apparatus in the asci. This detail, the absence of pigmentation in the spores and the abruptly cut base of the asci, leads you to the genus Valsa (Cytospora)
Vera Hayova is a good specialist who occasionally participates in this forum and who may be able to help you.
Good luck!
Eduard Osieck, 03-09-2020 10:07
Re : Eutypella (?) ... on Pinus.
Difficult group also because the literature is very scattered.

Many species are covered by the pyrenomycetes book of Munk (1957).

Leucostoma (Valsa) kunzei should have a black stromatic zone (or "conceptacle", see fig. 1g in Adams et al. 2005. Phylogenetic relationships and morphology of Cytospora species and related teleomorphs (Ascomycota, Diaporthales, Valsaceae) from Eucalyptus. Studies in Mycology. (52):1-146). 

Eduard
Enrique Rubio, 03-09-2020 12:16
Enrique Rubio
Re : Eutypella (?) ... on Pinus.
As you say, Leucostoma forms a blackish stromatized tissue (black line) surrounding the stromata and also a whitish tissue in the disc. Both things do not seem to be seen in your photos and therefore I do not think it is a Leucostoma.
But my knowledge of these mushrooms is very limited.
Eduard Osieck, 03-09-2020 13:01
Re : Eutypella (?) ... on Pinus.
Presumably, you are wright. Eduard
Peter Püwert, 03-09-2020 14:34
Peter Püwert
Re : Eutypella (?) ... on Pinus.
Hi, in my opinion is this Valsa pini. Unfortunately no microfotos.

Peter.
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Eduard Osieck, 03-09-2020 15:45
Re : Eutypella (?) ... on Pinus.
That could be wright, I wrongly assumed V. pini has larger ascospores.
Eduard