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Josep Torres Josep Torres

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Viktorie Halasu Viktorie Halasu

Hello, I keep finding something with spores like

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Geopyxis cf. delectans
Viktorie Halasu, 31-01-2017 06:51
Viktorie HalasuGood morning,
I was given exsiccate of this nice discomycete - could it be Geopyxis (Kotlabaea) delectans? Most characters agree, only the spores are a bit too small and no fireplace was noticed in the field.

A single apotecium about 1,5-2 cm diam., margin raised and a little brownish, no data about the outside in fresh state. Growing on the ground at the edge of a road, basic soil (on serpentinite), warm locality, no fireplace observed.
Microphoto here: http://tmp1.vize.name/myko/indet/ex439.jpg 


Excipulum two-layered, medúlla = t. intricata, ectal = t. angularis, about same thickness (up to 80-100 um). Surface cells sometimes covered with light yellow-brown exudate, surface looks like dotted. (The exudate is far too orange on the microphoto.)
Ascus base distinctly forked like in Pulvinula/Geopyxis spp.
Paraphyses filiform, straight, 2 um wide, almost not enlarged at the apex, sometimes branched in upper half, with distinct yellow granules inside even in exsiccate.
Spores smooth, ellipsoid with obtuse ends to almost cylindric, uninucleate, with a few little guttules when young, mature with granulose yellowish contents, (11.7)12–13.1(13.7) × (6.5)6.6–7.3(7.6) µm, Q = 1,7–1,9 (exs. in water).


Eguttulate spores exclude genus Acervus, non-capitate paraphyses and no distinct hairs exclude Pseudaleuria, Boubovia has smaller apo and curved paraphyses. Other Geopyxis spp should have bigger spores, but I saw only G. carbonaria so far.
Thank you in advance for your opinion.
Viktorie

  • message #46861
Uwe Lindemann, 31-01-2017 20:57
Uwe Lindemann
Re : Geopyxis cf. delectans
Hi Viktorie,
I would exclude G. carbonaria because these species is macros- and microscopically different.
G. delectans is an option althought the spores of your find are a bit too small. Also their form is a bit different (ellipsoid with sligthly tapered ends). Maybe the spores are immature? Mature spores of delectans are not thick-walled. Do you tested also a possible spore ornamentation with cotton blue in lactic acit? And do tested the reaction of the vacuoles in IKI? The reaction should be green.
The genus Geopyxis is difficult. As far as I know, in 2017 a new study about these genus will published, see here: https://www.ncbi.nlm.nih.gov/pubmed/27760850
Best, Uwe
Enrique Rubio, 31-01-2017 21:00
Enrique Rubio
Re : Geopyxis cf. delectans
Hi Uwe
Have you this article on Geopyxis? And in afirmative case, could you send me a pdf of it?
many thanks in advance
enrirubio@asturnatura.com
Uwe Lindemann, 31-01-2017 21:11
Uwe Lindemann
Re : Geopyxis cf. delectans
As far as I know is the paper not yet published.
Best, Uwe
Viktorie Halasu, 31-01-2017 21:25
Viktorie Halasu
Re : Geopyxis cf. delectans
Hello Uwe,
thank you for your answer. The spores were smooth in LACB, I'll check the IKI reaction later. 
About that article, I think I read something like that a preliminary version was already published, but the Mycologia website seems to be down for the moment. 

  • message #46870
Enrique Rubio, 31-01-2017 21:29
Enrique Rubio
Re : Geopyxis cf. delectans
Sorry, Uwe. You're are right!
Viktorie Halasu, 01-02-2017 22:36
Viktorie Halasu
Re : Geopyxis cf. delectans
Hello Uwe, 
I checked the iodine reaction of paraphyses, it's negative in both IKI and MLZ. But this collection is from 30.july 2016 and when I compared iodine reaction of Aleuria aurantia, collected in the beginning of august, it didn't react either. So I'm not sure, if it really excludes carotenoid presence. 

Nevertheless, what other genus could fit besides Geopyxis (and Pyropyxis)? No LBs, no ornamentation, no distinct hairs, dark orange color, and especially inamyloid asci with forked base, ... It's a bad luck there was just the one deformed apo, I hope they'll appear again this year.
  • message #46922