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Ciliolarina? on Juniperus in Southern Germany
Lothar Krieglsteiner, 30-12-2016 22:06
Lothar KrieglsteinerCollected on 29th of December, 2016 – on a decorticated piece of wood (root-lower stem) of Juniperus communis on a steep slope of dry heathland on calcareous soil (Jura). – near Heubach („Scheuelberg") in the mountain area Schwäbische Alb in Baden-Württemberg (Germany)

The white, minute apothecia are short-stalked to subsessile and minutely hairy under the lens. The excipulum of textura (angularis)-prismatica gives rise to cylindrical, finely rough hairs with some exudate on them. The asci have croziers and the minute porus reacts in IKI – but I am not really sure about the colour. I would call ist greyish-red, but my wife says it is more blue. The spores are small and have two larger oil drops.


Who  can tell me if I am right with Ciliolarina – and perhaps more?


Best regards from Lothar

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Stip Helleman, 31-12-2016 01:13
Stip Helleman
Re : Ciliolarina? on Juniperus in Southern Germany
Hi Lothar,
I think Cililarina is the most approprietive genus for your collection too, perhaps in the laetifica/neglecta group, did you observe any VBs in the paraphyses? They can turn Violet for a short while when IKI is added, this feature is observed in  neglecta and in ligniseda in a lesser part.

Stip
Hans-Otto Baral, 31-12-2016 09:46
Hans-Otto Baral
Re : Ciliolarina? on Juniperus in Southern Germany
Hi Lothar & Stip
I think it fits C. laricina quite well (see my folder). You can also try with Cresyl blue for the spore surface (pale lilc). Typical in C. laricina seems to be an rr/rb reaction of the ascus apical ring. Yours is surely dirty red, but maybe Katharina saw other asci with more blue rings under the microscope?
Zotto
Lothar Krieglsteiner, 31-12-2016 09:49
Lothar Krieglsteiner
Re : Ciliolarina? on Juniperus in Southern Germany

Hello Stip,


thanks for your answer!


I did not notice a violet reaction in the paraphyses but I wasn`t aware of this character, I must admit. I think the paraphyses have - quite poorly refractive - vacuolar bodies. I add another foto ...


The specimen is quite small and dry now - but I will take another apothecium for preparation in some days.


Thanks again and best wishes for 2017


Lothar

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Hans-Otto Baral, 31-12-2016 09:51
Hans-Otto Baral
Re : Ciliolarina? on Juniperus in Southern Germany
If you did not use a heat drier you could look for the drought tolerance, perhaps when you rewetten an apo it will still be fully alive.
Lothar Krieglsteiner, 31-12-2016 09:54
Lothar Krieglsteiner
Re : Ciliolarina? on Juniperus in Southern Germany

Hi Zotto,


thank you very much - our posts must have crossed :-)


So I will try also with Cresyl Blue - if I still find enough suitable apothecia in the specimen ...


Best regards -


und "einen guten Rutsch in ein gesundes, rundum positives Jahr 2017" (this was German)


Lothar - und Katharina

Stip Helleman, 31-12-2016 19:37
Stip Helleman
Re : Ciliolarina? on Juniperus in Southern Germany
Hi Lothar and Zotto,
the sporesize is in my opinion really too small for C. laricina, your collection fits fairly good to the extended Stokland descriptions of laetifica in Raitviir & Huhtinen 2002

Ein guten Rutsch!

Stip
Hans-Otto Baral, 31-12-2016 20:15
Hans-Otto Baral
Re : Ciliolarina? on Juniperus in Southern Germany
Hast recht Stip, obwohl mein Ordner von laricina auch Funde mit Sporen von 5-8 enthält (HB 8075): *5.7-7.7 x (1.8-)2-2.5(-3) µm (M. Hairaud: not over 7 µm).

I wasn't aware that Seppo suddently included samples with amyloide asci. But I still see some correlation between amyloid asci and a lower oil content in the spores. This group should be sequenced whether Seppo was right.

I worked a bit on my folders and included Seppo's specimens.

Zotto
Lothar Krieglsteiner, 01-01-2017 15:45
Lothar Krieglsteiner
Re : Ciliolarina? on Juniperus in Southern Germany

Hi Stip,


thank you - then I will change the name to laetifica.


By the way - do you have a digital copy of the paper of Raitviir & Huhtinen 2002?


Happy new year :-) - and best regards from


Lothar

Hans-Otto Baral, 01-01-2017 16:25
Hans-Otto Baral
Re : Ciliolarina? on Juniperus in Southern Germany
just sent it
Lothar Krieglsteiner, 01-01-2017 16:34
Lothar Krieglsteiner
Re : Ciliolarina? on Juniperus in Southern Germany

vielen Dank!


LG von Lothar

Stip Helleman, 01-01-2017 16:34
Stip Helleman
Re : Ciliolarina? on Juniperus in Southern Germany
Me too

Stip
Lothar Krieglsteiner, 01-01-2017 16:37
Lothar Krieglsteiner
Re : Ciliolarina? on Juniperus in Southern Germany

thanks for you also, dear Stip :-)


Yours, Lothar