Dear Joop,

the wood was quite wet, I presume? This is a Pseudohalonectria and the large ones are the spores. What you show as the fruitbody is only the neck. The main fruitbody is immersed in the wood. If you dig them out or make section through the wood you will find asci as well.

Regards
Martin

Thanks Martin,

 

The wood was indeed very wet due to the rain I will look for the fruitbody to check for the asci.

Reading the article of Shearer it probably is P. lignicola

 

Joop

Hi Joop,

I agree with Martin. At first, it could be useful to show an acomata, through a vertical section. Then, we can look at hamathecium.

I don't recognize for the moment Pseudohalonectria, but it's a possibilty.

Alain

Anamorfo. Volutella sp.?

I see what I can do Alain.

Joop

Here is a collection by Enrique:
http://www.asturnatura.com/especie/pseudohalonectria-lutea.html
http://www.asturnatura.com/fotografia/setas-hongos/pseudohalonectria-lutea-shearer/7294.html

Let's see what's comming up by Joop...

Regards
Martin

Found the fruitbody and the asci.
Fruitbody: 340 um in diameter; neck 266x84 um 

It looks like the asci are sticking out of the fruitbody like needles
 
Asci: 115x7.5 um; containing 8 spores.
Spores: 37x4 um; with 5 septa

I have found a second species but was not able to recover the fruitbody.

Neck: 680x98 um.
Spores: 34x3.5 um; it seems that some of the spores expand in size (photos 4&5)

Is this sufficient enough for you Martin or do you need more information?

 

Joop

Hi Joop,
this one is Fusarium

Regards,
Christian

Dear Joop,

Alains's comment made me unsure and I did wait for a further comment of him, since this is much more his world then mine. But he asked for a section of the ascocarp to check a hamathecium.
Anyway, the spores in your find do look much more acuminate as the published spores of Pseudohalonectria, where the ends are more rounded.  Yours are similar to a collection by me years ago (attached). This was not submersed but very wet wood as in your case I think.

Maybe it is something else in the Magnaporthaceae or elsewhere?

best regards
Martin

Hi all,

Last photos of Joop show two kinds of spores : first one acuminate which can't be Pseudohalonectria's one, you're right Martin, it was my idea too, they are conidiospores and Christian identifies them. But we can see second ones :  the ascospores, hyaline, multiseptate, fusiform. To describe more asci would be useful. Perhaps these two kinds of spores are independent.

Your macrophoto shows well ascomata, good photo Joop. Now I have no idea and no time but perhaps someone could recognize it.

Alain

Thanks very much all for your information.
I did put in another species I found today in a new topic maybe that one is better to identify.

I will have a look if I can find a new one for more info.

 

 

Joop

Thanks to you Joop. A such research is interesting for everybody.

Alain

Hmm,

I am not convinced with Fusarium. At least the spores of my collection were born from Asci.

Regards
Martin

Hello Martin,

I do think Martin the 2 spores are different, today I found 3 more species and the length and width of the ascospores do have a different range. But it is also possible that these drops of the ascospores merge to one within the boundary of their septa.
Spores near the neck: 31.5-37x3.5-4.0 um; with 4 large oil drops and 5 septa.
Ascospores: 41.5-45x3.5-4.5 um; with 9 large oil drops the number of septa could not be observed.

Photo-3&4 shows a young dark brown ascomata with neck attached to the neck of the bigger one, no spores found

Asci still have 8 spores that are clustered in 2 bundles of 4 ascospores. The bundles are shown in photo-9.

The asci are normally shorter than shown, what shows is the ascus with the bundles photo-8. On photo-09 tops of these asci are sticking out of the bottom of the ascomata. Size of these asci: 120-130x10.5-11.5 um.
When pressure on the deck glass is increased the asci extend in length as shown in photo-10. The size of this ascus is 160x11.5 um and the top bundle is still there.

The ascomata of this species is white in contrast with the other species with a long neck I found having a black ascomata and totally different ascospores.

Hi,

this is Conioscyphascus varius.

regards,
björn

Septation only visible in IKI (Melzer)

Did you check if there is a dead Eutypa nearby?

Hi Björn,

the description of Réblová&Seifert of Conioscyphascus varius doesn't make me finally happy. The neck of the ascocarps is very short and the lenght-width ratio of the spores seems to be wider. But this seems to be the right corner to look after our fungi in question. Thanks!

Regards
Martin

Not to my knowledge Björn.

Thanks for the info, do you have an idea what the name is of the species I found in the topic of 27/11. Has the same kind of neck but a black ascomata and totally different ascospores.

 

Joop

Hi Martin,

I have the luck to had studied several collections from both Conioscyphascus-species from The Netherlands (a collaboration with Eduard Osiek). Both species seem to be variable in spore size, as also in the shape of ascomata (perithecial neck, even perithecia size).

We came to the conclusion that all the collections < 40µm belong to C. gracilis, and all others to C. varius, which has probably two appearances (C. varius with short spores around 38-46µm, and a C. varius with long spores around 45-52µm).


If the average length of the spores is over 40µm, I am quite sure that this collection here is C. varius.

regards,
björn

Ok Björn,

in both of our collections spore length is below 38 µm. So it is C. gracilis?

Regards
Martin

I do not know which spore length is the correct one, as there are given several sizes, one also over 40micrometer. If the length is constantly not over 40, then it is gracilis.

C. gracilis is even more short necked (according to the description by Réblová&Seifert). I did not measure many spores, but those were 34-37x3.3-3.7µm. The perithecia should be mostly superficial as well. I would exclude gracilis and think I missed to find longer spores....

My résumé is: next time I find this, I know what to look upon... ;-)

Best regards

Martin

Hello Martin and Björn,

I will look if I can find another species today and measure a lot of spores.

 

Joop

Hi Joop. I would be happy if you can send me a collection of your Conioscyphascus.

Regards. Björn

Hi Björn,

after Melbourne it is now Conioscypha:
http://www.sciencedirect.com/science/article/pii/S1340354014000898
since the anamorph has the older name.

regards
Martin

Oh no, not again arrr thanks Martin

I see what I can do for you Björn, today I found them again together with two other species with ascomata embedded in the dung. Maybe I can provide you with all three species.
Spore measurement for Conioscypha:
42,5-48x3.0-4.0 um. According to the article Martin mentioned it can only be C. varius because of its long neck and spore size >35 um according to the key.
One of the other species also has a long neck but different kind of spores I posted the topic on the 27th of november. No response so no name yet.
For the second one I will start a new topic it has a very small neck.

 

Number of septa in ascospores is 5.
The last two photos show that the ascomata has a more oval shape (401x301 um)

 

Joop