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09-08-2022 20:06

Stephen Mifsud Stephen Mifsud

I have found an interesting aspergillus on flowers

06-08-2022 22:20

Marcus Yeo

I found this ascomycete today on dead leaves of Er

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Zetti Mario

Hi, I am doing some work with microfungi and durin

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Pierre-Yves Julien

Récolte le 31/07/2022 – Ozoir-la-Ferrière (77)

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Louis DENY

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Lothar Krieglsteiner Lothar Krieglsteiner

near Snasa, on mixed lawn with Nowellia curvifolia

05-08-2022 08:43

Josep Torres Josep Torres

Hola.Un líquen sobre la corteza de un tronco de p

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Stefan Jakobsson

A light-coloured Hymenoscyphus growing on a cone o

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Josep Torres Josep Torres

Hola.Un liquen cortícola fotografiado este pasado

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Lothar Krieglsteiner Lothar Krieglsteiner

on decorticated branch of surely Betula (as there

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Cryptodiaporthe?
Enrique Rubio, 29-03-2013 13:22
Enrique RubioHi to all
This fungus makes isolated, imersed, blackish perithecia up to 0.4 mm in diameter with very inconspicuous ostioles on small branches of an indeterminate deciduous tree (maybe Salix).
I think in a Cryptodiaporthe species and my guest is C. salicella, but my ascospores and asci are greater than the Wehmeyer's description. On the other hand this species seems to be very variably described in the literature.
What do you think?  

Thanks again
Enrique
  • message #22732
  • message #22732
  • message #22732
Hans-Otto Baral, 29-03-2013 16:19
Hans-Otto Baral
Re : Cryptodiaporthe?
What is the staining reagent here, Congo Red and Cresyl blue?

Zotto
Alain GARDIENNET, 29-03-2013 16:31
Alain GARDIENNET
Re : Cryptodiaporthe?
Salut l'ami Enrique,
Very interesting.
Probably a Plagiostoma species. (You know perhaps  that several species like salicellum have been placed in Plagiostoma genus, Meijia & al.2011).
There are some species on Salix, most of them in North America.  But even if your record is closed to Plagiostoma salicella, the ascospores are too much long to recognize it here.
How is the neck ? Is there a white area around ostiole ?
Alain
Alain GARDIENNET, 29-03-2013 16:33
Alain GARDIENNET
Re : Cryptodiaporthe?
You cited Wehmeyer. In its workshop, the closest species to yours is Diaporthe megalospora , but it's on Sambucus and really different too.
Alain GARDIENNET, 29-03-2013 16:35
Alain GARDIENNET
Re : Cryptodiaporthe?
And how many perithecia for one stroma ?
Alain GARDIENNET, 29-03-2013 16:37
Alain GARDIENNET
Re : Cryptodiaporthe?
I think that you should contact Donald Walker, working actually on Plagiostoma genus :
walkerd@findlay.edu

China, America have new species on Salix. Why not Europe ?
Best regards,

Alain
Alain GARDIENNET, 29-03-2013 16:52
Alain GARDIENNET
Re : Cryptodiaporthe?
You will find the doc (the more actual on this genus for the moment), in an old post in this forum :

http://www.ascofrance.fr/search_forum/14961
Alain
Enrique Rubio, 29-03-2013 17:14
Enrique Rubio
Re : Cryptodiaporthe?
Thank you to all

Zotto, the staining reagent were congo red and nigrosine 


The 1-peritheciate fruit bodys are very scattered on the branch. No strue stroma I have seen. No ventral black line. No white line around the very inconspicuous ostiole. I cannot see well the neck.
Thanks!
Hans-Otto Baral, 29-03-2013 17:21
Hans-Otto Baral
Re : Cryptodiaporthe?
Enrique:

nigrosin I though has a black colour. And it provokes a blue ring?

I assume that nigrosin works like a basic dye:
"Nigrosin WS is used in tests for viability: living cells exclude the dye, but it enters dead cells"
(wikipedia)

Zotto
Enrique Rubio, 29-03-2013 18:42
Enrique Rubio
Re : Cryptodiaporthe?
Nigrosine has a blackish/bluish color under the microscope but perhaps the photoshop changes include more bluish hues to the apical apparatus.