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03-07-2020 17:47

Thomas Læssøe

see: https://svampe.databasen.org/observations/10

27-06-2020 13:21

Hardware Tony Hardware Tony

I wondered if anyone could help me with this ascom

04-07-2020 14:34

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello, I would like to ask you for help with idet

03-07-2020 21:06

Castillo Joseba Castillo Joseba

me mandan el material seco,, recolectado en plante

03-07-2020 20:54

Castillo Joseba Castillo Joseba

me mandan el material seco recolectado en rama de

03-07-2020 22:51

Viktorie Halasu Viktorie Halasu

Hello,I'd like to ask for help with a lichen. In w

02-07-2020 21:45

Castillo Joseba Castillo Joseba

me mandan el material seco, recolectado en  tallo

02-07-2020 07:56

Garcia Susana

Hola, Agradecería vuestra ayuda para la identifi

02-07-2020 13:33

Garcia Susana

Hola, He recogido estos apotecios que crecían en

02-07-2020 16:05

Thomas Læssøe

see: https://svampe.databasen.org/observations/10

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Cryptodiaporthe?
Enrique Rubio, 29-03-2013 13:22
Enrique RubioHi to all
This fungus makes isolated, imersed, blackish perithecia up to 0.4 mm in diameter with very inconspicuous ostioles on small branches of an indeterminate deciduous tree (maybe Salix).
I think in a Cryptodiaporthe species and my guest is C. salicella, but my ascospores and asci are greater than the Wehmeyer's description. On the other hand this species seems to be very variably described in the literature.
What do you think?  

Thanks again
Enrique
  • message #22732
  • message #22732
  • message #22732
Hans-Otto Baral, 29-03-2013 16:19
Hans-Otto Baral
Re : Cryptodiaporthe?
What is the staining reagent here, Congo Red and Cresyl blue?

Zotto
Alain GARDIENNET, 29-03-2013 16:31
Alain GARDIENNET
Re : Cryptodiaporthe?
Salut l'ami Enrique,
Very interesting.
Probably a Plagiostoma species. (You know perhaps  that several species like salicellum have been placed in Plagiostoma genus, Meijia & al.2011).
There are some species on Salix, most of them in North America.  But even if your record is closed to Plagiostoma salicella, the ascospores are too much long to recognize it here.
How is the neck ? Is there a white area around ostiole ?
Alain
Alain GARDIENNET, 29-03-2013 16:33
Alain GARDIENNET
Re : Cryptodiaporthe?
You cited Wehmeyer. In its workshop, the closest species to yours is Diaporthe megalospora , but it's on Sambucus and really different too.
Alain GARDIENNET, 29-03-2013 16:35
Alain GARDIENNET
Re : Cryptodiaporthe?
And how many perithecia for one stroma ?
Alain GARDIENNET, 29-03-2013 16:37
Alain GARDIENNET
Re : Cryptodiaporthe?
I think that you should contact Donald Walker, working actually on Plagiostoma genus :
walkerd@findlay.edu

China, America have new species on Salix. Why not Europe ?
Best regards,

Alain
Alain GARDIENNET, 29-03-2013 16:52
Alain GARDIENNET
Re : Cryptodiaporthe?
You will find the doc (the more actual on this genus for the moment), in an old post in this forum :

http://www.ascofrance.fr/search_forum/14961
Alain
Enrique Rubio, 29-03-2013 17:14
Enrique Rubio
Re : Cryptodiaporthe?
Thank you to all

Zotto, the staining reagent were congo red and nigrosine 


The 1-peritheciate fruit bodys are very scattered on the branch. No strue stroma I have seen. No ventral black line. No white line around the very inconspicuous ostiole. I cannot see well the neck.
Thanks!
Hans-Otto Baral, 29-03-2013 17:21
Hans-Otto Baral
Re : Cryptodiaporthe?
Enrique:

nigrosin I though has a black colour. And it provokes a blue ring?

I assume that nigrosin works like a basic dye:
"Nigrosin WS is used in tests for viability: living cells exclude the dye, but it enters dead cells"
(wikipedia)

Zotto
Enrique Rubio, 29-03-2013 18:42
Enrique Rubio
Re : Cryptodiaporthe?
Nigrosine has a blackish/bluish color under the microscope but perhaps the photoshop changes include more bluish hues to the apical apparatus.