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 Lothar Krieglsteiner
                Lothar Krieglsteiner
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 Maria Plekkenpol
                Maria Plekkenpol
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 Nicolas Suberbielle
                Nicolas Suberbielle
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 Stephen Martin Mifsud
                Stephen Martin Mifsud
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 Bernard Declercq
                Bernard Declercq
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Carl FarmerI'd be grateful for any suggestions for this strik
 
                                    28-10-2025 11:29
 Tanja Böhning
                Tanja Böhning
                Hello, I found this very small (ca 0,5mm) yellow
Unknown purple-black asco, long spores, brown epithecium
    
                    Ethan Crenson,
                21-09-2025 14:49
    
    Hi all, 
I am at a foray in central New York state.  Yesterday afternoon I found an intersting asco on hardwood in a dry stream bed growing amidst a group of orange ascos (probably Helotiales). They are purple black, smaller than 1/2 mm in diameter, barrel shaped.
The spores are long and multiseptate. They measure about 83-93 x 2.8-3.3µm.  Asci are thick walled, IKI-.  Paraphyses are filiform, septate, branching. 
There is a epithecium which, in water, appears to consist of a brown irregular debris in the mount.
Can anyone place this in a genus?  I am uncertain.
Ethan
    
    
    
        
                                    Hans-Otto Baral,
                                21-09-2025 16:55            
             
                Re : Unknown purple-black asco, long spores, brown epithecium
                Hi Ethan,
Uff, this is very interesting. I guess it could be Claussenomyces tympanoides. That species releases a purplish-red pigment when KOH is added to a water mount.
We urgently need a sequence of this species since we have a similar European taxon which seems to be a new species.
How abundant is the sample? If you want to try the KOH reaction (inonomidotic) please use a small apo in order to safe material for DNA.
Zotto 
                                    Ethan Crenson,
                                22-09-2025 03:17            
            Re : Unknown purple-black asco, long spores, brown epithecium
                Hello Zotto, Thank you for your reply. I don't have very much of the material.  I just returned home and am drying one apothecia for my own attempt at sequencing.  I will try the KOH reaction tomorrow. If you would like the rest of the specimen I can send it. 
Ethan
                                    Hans-Otto Baral,
                                22-09-2025 09:13            
             
                Re : Unknown purple-black asco, long spores, brown epithecium
                Let's wait for Adam and Luis, Maybe Adam wants it. If you think you can generate an ITS sequence, this would give us a great hint. Adam's presumed new species is in GenBank with ITS and LSU. It falls very apart from any other described species. I assume your lab uses ITS1F as primer for ITS, because Adam's species possesses the intron in the SSU.
                
                
                
                
                
                            
                                    Adam Polhorský,
                                23-09-2025 08:29            
            Re : Unknown purple-black asco, long spores, brown epithecium
                Wow incredible, first you have found sister species to I. uncinata, now possibly the type of the genus. From yours aff. uncinata we have a nice culture. I can attempt the same here. I will welcome any material. Luis examined the type of C. tympanoides, and it was too old to sequence,  your sample would be a great help.
Thank you, 
Adam.
 
                









