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31-10-2025 09:19

Lothar Krieglsteiner Lothar Krieglsteiner

Can somebody provide me with a file of:Rogerson CT

30-10-2025 03:53

Ethan Crenson

Hi all,  I would like an opinion on whether this

09-08-2025 13:13

Maria Plekkenpol Maria Plekkenpol

Hello,Yesterday I found these on burnt soil. Apoth

28-10-2025 19:33

Nicolas Suberbielle Nicolas Suberbielle

Bonjour à tous,Je voudrais votre avis sur cette r

29-10-2025 19:02

Castillo Joseba Castillo Joseba

De la pasada semana en rama posiblemente de hayaPi

25-11-2016 13:54

Stephen Martin Mifsud Stephen Martin Mifsud

Hi, I found numerous seeds of Washingtonia robusta

28-10-2025 22:22

Bernard Declercq Bernard Declercq

Hello.I'm searching for the following paper:Punith

27-10-2025 19:51

Peter Welt Peter Welt

Who has this article? Doveri, F. 2007. Sporormiel

28-10-2025 15:37

Carl Farmer

I'd be grateful for any suggestions for this strik

28-10-2025 11:29

Tanja Böhning Tanja Böhning

Hello, I found this very small (ca 0,5mm) yellow

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Unknown purple-black asco, long spores, brown epithecium
Ethan Crenson, 21-09-2025 14:49
Hi all,

I am at a foray in central New York state.  Yesterday afternoon I found an intersting asco on hardwood in a dry stream bed growing amidst a group of orange ascos (probably Helotiales). They are purple black, smaller than 1/2 mm in diameter, barrel shaped.

The spores are long and multiseptate. They measure about 83-93 x 2.8-3.3µm.  Asci are thick walled, IKI-.  Paraphyses are filiform, septate, branching.

There is a epithecium which, in water, appears to consist of a brown irregular debris in the mount.

Can anyone place this in a genus?  I am uncertain.

Ethan
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Hans-Otto Baral, 21-09-2025 16:55
Hans-Otto Baral
Re : Unknown purple-black asco, long spores, brown epithecium
Hi Ethan,

Uff, this is very interesting. I guess it could be Claussenomyces tympanoides. That species releases a purplish-red pigment when KOH is added to a water mount.

We urgently need a sequence of this species since we have a similar European taxon which seems to be a new species.

How abundant is the sample? If you want to try the KOH reaction (inonomidotic) please use a small apo in order to safe material for DNA.

Zotto 
Ethan Crenson, 22-09-2025 03:17
Re : Unknown purple-black asco, long spores, brown epithecium
Hello Zotto, Thank you for your reply. I don't have very much of the material.  I just returned home and am drying one apothecia for my own attempt at sequencing.  I will try the KOH reaction tomorrow. If you would like the rest of the specimen I can send it. 

Ethan
Hans-Otto Baral, 22-09-2025 09:13
Hans-Otto Baral
Re : Unknown purple-black asco, long spores, brown epithecium
Let's wait for Adam and Luis, Maybe Adam wants it. If you think you can generate an ITS sequence, this would give us a great hint. Adam's presumed new species is in GenBank with ITS and LSU. It falls very apart from any other described species. I assume your lab uses ITS1F as primer for ITS, because Adam's species possesses the intron in the SSU.
Adam Polhorský, 23-09-2025 08:29
Re : Unknown purple-black asco, long spores, brown epithecium
Wow incredible, first you have found sister species to I. uncinata, now possibly the type of the genus. From yours aff. uncinata we have a nice culture. I can attempt the same here. I will welcome any material. Luis examined the type of C. tympanoides, and it was too old to sequence,  your sample would be a great help.

Thank you, 

Adam.
Ethan Crenson, 23-09-2025 17:18
Re : Unknown purple-black asco, long spores, brown epithecium
Here is a photo of the ionomidotic reaction. It was quite strong initially, but it took me a moment to get my camera (it was still packed). 
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