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Hymenoscyphus?
Josep Torres, 07-09-2025 08:19
Josep TorresHello.
Tiny pinkish discomycetes, photographed and collected on August 17, sprouting scattered on the surface of a fallen spruce (Abies alba) trunk.
Apothecia attached to the substrate only by the central part, without a distinct stem, with a diameter between 1.17 and 1.75 mm, whitish with pinkish tinges.
The medullary exciple is filamentous, with an intricate texture, and the ectal exciple has a globose-angular texture.
Short, septate marginal hairs with rounded ends.
Octosporic asci, in some cases with uncinules.
The paraphyses are septate, filiform, unbranched, and protrude very slightly above the level of the asci.
No significant reaction of the asci with Melzer's reagent has been observed.
Cylindrical ascospores, with one or two large lipid droplets inside and other smaller ones scattered throughout. Between one and three septa can be discerned in some of these ascospores, with measurements in water of:
(11.5) 12.5 - 15.2 (15.6) × (3.8) 4 - 4.8 (5) µm
Q = (2.4) 2.9 - 3.6 (4) ; N = 30
Me = 13.9 × 4.3 µm ; Qe = 3.2

These specimens were found very close to what appears to be Hymenoscyphus imbrebis (there were three specimens on a nearby trunk), so at first I thought they were probably the same, although the microscopy doesn't match at all. Based on its microscopic characteristics, I'm thinking of Calycellina, and within Calycellina, the best fit is Calycellina subtrabinella, but there are too many conflicting characteristics, such as the host, which I haven't been able to find information on whether it can grow on coniferous wood, and the lack of reaction to Melzer's, as well as the spore width, don't agree.
Any feedback from you would be welcome.
Thank you in advance.
Best regards.

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Josep Torres, 07-09-2025 08:21
Josep Torres
Re : Hymenoscyphus?
The rest of the images:
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Hans-Otto Baral, 07-09-2025 09:01
Hans-Otto Baral
Re : Calycellina?
The elements are mostly not alive, so it is not easy. VBs are present, although I saw them in only one paraphysis (the yellowish-reddish colour is due to oxidation of these VBs).

Melzer is a bad reagent for Helotiales asci, so I am not sure if the asci are inamyloid. Maybe.

My guess is a member of the Hymenoscyphus imberbis group.
Josep Torres, 08-09-2025 14:41
Josep Torres
Re : Hymenoscyphus?
Thanks, Zotto.
I mentioned in my thread that these apothecia sprouted right next to what I considered the typical Hymenoscyphus imberbis.
I'm adding a few images from my study of what I believe to be Hymenoscyphus imberbis, growing right next to it. In this case, a faint amyloid reaction of the apical apparatus is visible, along with many paraphyses with contents, and the uncinules at the base of the asci.
In my study, the spores measured 13.1 - 16.05 (16.1) × (5.1) 5.15 - 6.3 µm, with a width well above the previous ones (3.8) 4 - 4.8 (5) µm. It is because of this data that I have come to doubt that it was the same thing.
I'll leave both studies as Hymenoscyphus imberbis sl.
Thank you again for your opinion.
Best regards,
Josep.
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Josep Torres, 08-09-2025 14:43
Josep Torres
Re : Hymenoscyphus?
The rest of the images:
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Hans-Otto Baral, 08-09-2025 15:24
Hans-Otto Baral
Re : Calycellina?
A spore width of up to 6 µm would be unusual for H. imberbis, also for H. kathiae which I otherwise would apply to both of your docus. The large LBs in the spores exclude H. imberbis.
Josep Torres, 09-09-2025 08:03
Josep Torres
Re : Hymenoscyphus?
Thanks, Zotto.
So, if it's not Hymenoscyphus imberbis or Hymenoscyphus kathiae, is there another option within Hymenoscyphus that fits my proposals? Or should I just file it as Hymenoscyphus sp.?
Best regards.
Hans-Otto Baral, 09-09-2025 09:10
Hans-Otto Baral
Re : Hymenoscyphus?
Your first posting seems to me H. kathiae. Your secodn needs free spores, best with a scale, to make up my mind.
Josep Torres, 09-09-2025 14:46
Josep Torres
Re : Hymenoscyphus?
Thanks, Zotto.
I'll try to recover the exsiccata and gather new data. My mistake was that, since it grew on practically the same trunk, I assumed it was probably the same thing, at different stages of development.
Best regards.
Josep Torres, 22-10-2025 08:39
Josep Torres
Re : Hymenoscyphus?
Hello Zotto.
Sorry for the delay. Since I couldn't obtain spores, I sent one of the specimens for sequencing. The ITS gene result determined that, just as I suspected, it was a 100% match for Hymenoscyphus kathiae.
Best regards.
Hans-Otto Baral, 22-10-2025 11:46
Hans-Otto Baral
Re : Hymenoscyphus?
Great! There are several European sequences of H. kathiae which are fully identical (most of them so far not in GenBank). But since some anamorph sequences appear with 98.3% similarity in Blast search, your sequence would be valuable to publish. Would you mind sending it to me, together with the collection data? If you got the ab1 file I would check that too.

From which group of apothecia does the sequence comes? From the first with the normal spore width or from the second wth unusually broad spores?

Zotto
Josep Torres, 23-10-2025 07:53
Josep Torres
Re : Hymenoscyphus?
Hello Zotto.
You already have everything in your email.
Best regards.