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Mot de passe perdu? S'inscrire

12-08-2010 13:28

Pyreno #2
Gernot Friebes

Hi again, here is the second find by my friend.

12-08-2010 12:35

Leptosphaeria eustoma?
Gernot Friebes

Hi, a friend of mine sent me two pyrenos which

10-08-2010 19:02

Psilachnum? on Polytrichum
Marja Pennanen

Hello, for a while I've been wondering this tiny

08-08-2010 15:34

On Potentilla
Marja Pennanen

Hello, once again I'm lost in the jungle of new s

07-08-2010 15:51

It's been hot and dry here
Marja Pennanen

So I've been forced to seek damp places. The botto

06-08-2010 22:22

Peziza saccardiana?
Jean Pierre Dechaume Jean Pierre Dechaume

Peziza de 2-3 cm, sur terre nue avec jeunes mousse

06-08-2010 00:51

Literature search... Can.J.Bot.
Martin Bemmann Martin Bemmann

Hi all, does someone have a copy of Redhead's p

03-08-2010 01:26

Preintroducing Hyaloscypha huhtinennii
Marja Pennanen

Hello folks, I found something on mosses (so I th

02-08-2010 23:49

Technical question regarding Bombardia
Martin Bemmann Martin Bemmann

Hi, did someone of you try to make a slide of B

31-07-2010 00:09

Can it be?
Marja Pennanen

I think, that I finally managed to determine one H

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Orange disks
Leif Goodwin, 14-10-2023 11:29
This summer I found some cup fungus on soil with acer and black poplar. I struggled to get a spore print so only got a few spores. The asci and paraphyses are shown in Melzers, the spores in Lacto Cotton Blue. The disks are up to 2 cm wide. 

Someone suggested a Purpureodiscus species e.g. P. isabellinus. 

Help appreciated.
  • message #77143
  • message #77143
  • message #77143
Nicolas VAN VOOREN, 22-10-2023 11:19
Nicolas VAN VOOREN
Re : Orange disks
Macroscopically it looks like Purpureodiscus subisabellinus, but you should show some mature ascospores (in water). They measure 18.5-23.5 × 11-13 µm and show bipolar spore granules. The amyloid reaction of asci is only visible on the wall (W type), sometimes difficult to see.
Leif Goodwin, 13-11-2023 18:43
Re : Orange disks
Thank you for taking the time to reply, and apologies for my late response. I have given the specimen to someone who will amplify the ITS region of the DNA and then have it sequenced. When he returns it, I will try and find some mature spores. Hopefully the DNA sequencing will give us an answer, but sadly DNA does not always amplify, possibly because the primers are not ideal for ascos.