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Minute apos on bark of oak

Stefan Jakobsson, 03-03-2023 20:13

On a branch of Quercus on the underside of loose bark and in bark cracks there were some minute apothecia, turbinate, 0,13-0,22 mm in diameter. The asci are 51-67 × 8.5-11.3 µm, IKI negative. The spores are 10.1-13.0 × 3.2-3.5, occasionally septate. Paraphyses cylindrical, 1-2 µm, thin-walled, septate, branched. Excipular hairs cylindrical, 1 µm thick, thin-walled, slightly amyloid in Melzer and a few marginal hairs seem to have some lumps turning black.

I would be grateful for suggestions on this one. Thanks!

Kosonen Timo, 03-03-2023 21:37

Kosonen Timo

Re : Minute apos on bark of oak

Hi,

Are they hairs for sure? I suppose they are then. Hyaloscypha daedalea would be my starting point. But the spores are rather large here. And H. quercicola has MLZ- asci. WOuld that be an option?

I dont know. If I get it right the dextrinoid part in the hairs is quite long. Howabout Hyaloscypha intacta? The hair layout would fit. H. intacta is often very anonymous macroscopically.

Timo

Stefan Jakobsson, 03-03-2023 21:57

Re : Minute apos on bark of oak

I got a better view of the hairs in CR - the shape could fit e.g. intacta. Actiually I found what I take to be H. intacta on the same branch but that is macroscopically very different, sessile and much more dextrinoid:

http://tun.fi/JX.1534214#3

Kosonen Timo, 03-03-2023 22:08

Kosonen Timo

Re : Minute apos on bark of oak

kind of large hairs for intacta. Are the asci simple septate? ...Then we have H. spiralis, simple septa, hairs and spores would fit. Excpular cells dextrinoid by any chance?

Stefan Jakobsson, 03-03-2023 22:20

Re : Minute apos on bark of oak

Just slightly dextrinoid. I have difficulties making up my mind on croziers.

Stefan Jakobsson, 03-03-2023 23:21

Re : Minute apos on bark of oak

No bluing whatsoever of the ascal plug even after KOH pretreatment. That leaves only H. intacta then, I presume.

H. intacta seems to be very common during the winter months, but I have never seen it like this. All intactas I have seen have been on branches recently fallen from the canopies - like this one. This fungus had a very light brownish or beige colour which I have never noticed before. My intactas have always been whitish or greyish hyaline. Likewise, the substipitate apos are new - I have always seen only sessile apos of intacta. Seppo says "...narrowly to broadly sessile".

Danke schön!

Kosonen Timo, 04-03-2023 08:53

Kosonen Timo

Re : Minute apos on bark of oak

I think there's always room for doubt with Hyaloscyphas. I am getting confused with intactas often. There's usually atleast one sample per year labeled "small hyaloscyphoid, no hairs" or something like that, only turning out to be a H. intacta once sequenced. Then, going back to scope, there's the single hair somewhere with dextrinoid apex. Usually dextrinoid reaction (excipulum) is more or less clear. Maybe that's the questionmark here?

Timo