11-05-2025 17:57
Louis DENYHello forumTrouvé dans les environs de Belfort,al
08-05-2025 20:50
Andreas JacobGood evening, due to contstant drought I started
11-05-2025 10:22
Karl Soler KinnerbäckFound on moist Betula wood dipped in freshwater st
11-05-2025 10:35
ruiz JoseHola, en excremento de jabali, tamaño de unos 2 m
05-05-2025 10:09
Nicolas Suberbielle
Re-bonjour,Cet ascomycète trouvé et étudié par
09-05-2025 16:01
Thomas FlammerI found a black cushon which might eventually be
09-05-2025 19:10
Enrique Rubio
Hi to everybody.This Orbilia (apos up to 0.5 mm),
06-05-2025 13:37
Thomas FlammerMunk, Anders (1953). The System of the Pyrenomycet
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie