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29-03-2024 14:37

Michel Hairaud Michel Hairaud

Bonjour , Voici une nouvelle récolte de cette be

29-03-2024 09:21

Michel Hairaud Michel Hairaud

Bonjour, Je recherche la description du genre Mac

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A rhizodiscina-like collection
Michel Hairaud, 28-04-2022 23:49
Michel HairaudBonjour, 
Je viens de recevoir cette récolte aux apothécies noires , stipitées, larges de 3-5 mm. 

Asques aux parois amyloïdes, 95-100 x 7-8 µm. ectal de cellules rondes à parois brun foncé . Spores hyalines, septées, biguttulées, et non lisses (verrues très petites et peu visibles) 
11-13 x 4,5-5 µm
Paraphyses septées , élargies au sommet et recouvertes d une matière amorphe brune. 

Merci pour toute indication de piste de détermination. Amitiés
Michel
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Hans-Otto Baral, 29-04-2022 08:00
Hans-Otto Baral
Re : A rhizodiscina-like collection
Hi Michel

very strange thing! I am sure you mean 3-5 mm, so quite a large fungus. On what kind of wood did it grow? I assume on the moist ground?

To me this looks like a Patinella, I assume Luis Quijada would be interested.

Zotto
Michel Hairaud, 29-04-2022 08:23
Michel Hairaud
Re : A rhizodiscina-like collection
Thanks, Zotto, 
Yes of course 3-5 mm large apos. I will correct it

My Ref : MH 80422

I wanted to mention that none of the apos have a proeminet margin, which should be a usual character in Patellaria. 

Michel
Hans-Otto Baral, 29-04-2022 09:32
Hans-Otto Baral
Re : A rhizodiscina-like collection
I said Patinella, but I am wrong, Patinella is inamyloid. Compare Pseudosclerococcum golindoi. Sclerococcum is the new name for Dactylospora, and Pseudosclerococcum is a related genus in which the asci do not have the pronounced amyloid apical cap but react more diffusely along the entire ascus wall.

Tell ,me if you have access to it:

Olariaga, I.; Teres, J.; Martín, J.; Prieto, M.; Baral, H.O. (2019). Pseudosclerococcum golindoi gen. et sp. nov., a new taxon with apothecial ascomata and a Chalara-like anamorph within the Sclerococcales (Eurotiomycetes). Mycological Progress 18: 895–905. - https://doi.org/10.1007/s11557-019-01500-7
Michel Hairaud, 29-04-2022 13:29
Michel Hairaud
Re : A rhizodiscina-like collection
This is the good hint, Zotto ! 

The main difference (amyloid ascus walls ) may not be an obstacle as this reaction is very fleeting (hardly a few minutes ) though systematic and clear, and easier to observe on thicker preps. (and none on the apex or chamber) 
Then the hemiamyloid reaction is the rule. 
The ascus dehiscence is the very same as described in the mentioned paper. ... 
As to the ectal it is actually mainly globosa in my collection

I also think I can observe a chalara-like asexual morph on the substrate.
Michel
Hans-Otto Baral, 29-04-2022 16:38
Hans-Otto Baral
Re : A rhizodiscina-like collection
Did you also photograph the anamorph?

I compared spore size which fits very well. Surely it is this species.

Now I wish to know the substrate, any idea? And geographical origin?
Michel Hairaud, 01-05-2022 08:13
Michel Hairaud
Re : A rhizodiscina-like collection
Happy May day to all, 

The Chalara-like observed on the substrate does not look like the one in the quoted paper. (Obtained in culture as I understood) 
There is no certainty about the substrate . Could be oak or beech according to my correspondant. 
Please see cross section pics. 
The fleetin reaction is indeed systematic and lasts only a very few minutes. 

Collection date : France, Manche, (50) St Sauveur Le Vicomte, forêt de ST Sauveur. 23 04 2022 on a decaying log. 
Amitiés
Michel


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Hans-Otto Baral, 01-05-2022 20:48
Hans-Otto Baral
Re : A rhizodiscina-like collection
I would not say chalara-like to this, and who knows if it belongs to the species.

The wood is obviously no Quercus, but it is necessary to use a lower magnification to see the pores for at least one entire year of growth.

Yes, this IKI RB reaction is just what is described in the paper.
Michel Hairaud, 03-05-2022 15:09
Michel Hairaud
Re : A rhizodiscina-like collection
Hi Zotto, 
I agree, the asexual morph found on the substrate is not necessary linked with the Pseudosclerococcum presence.
As to the IKI reaction, I may have not been clear : the blue fleeting reaction has been observed whithout KOH pretreatment, which I do not see in the  princeps description. 

The piece of substrate received is unfortunately too thin and decayed to get a section showing at least  a year's growth . 

After the description, it seems that the species was collected several times on the same log . I will ask my correspondant to try new collections after long rainy periods. 
Amitiés. Michel
Hans-Otto Baral, 03-05-2022 21:34
Hans-Otto Baral
Re : A rhizodiscina-like collection
Ah I see, the protologue says IKI red, type RR, and you observed type RB!

Good! Maybe variation.
Bernard Declercq, 10-05-2022 12:52
Bernard Declercq
Re : A rhizodiscina-like collection
Hi Zotto,

I am interesting receiving mentioned paper. Thanks in advance.

Bernard
Michel Hairaud, 10-05-2022 14:01
Michel Hairaud
Re : A rhizodiscina-like collection
Sent to your mail box
Michel