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Mot de passe perdu? S'inscrire

16-04-2024 17:43

Peziza vesiculosa ???
Giovanni ANTOLA Giovanni ANTOLA

Bonjour,Trouvé sous paille humide, autour d'un je

14-04-2024 20:04

Gyromitra parma?
Manak Roman

Hi all,I have two very similar finding last weeken

17-04-2024 10:44

Lachnum virgineum sur cône de pin sylvestre à confirmer
Bernard CLESSE Bernard CLESSE

Juste à côté du cône avec "Hyphodiscus ayelii"

16-04-2024 22:53

Hyaloscyphaceae sur cône de pin sylvestre (Pinus sylvestris)
Bernard CLESSE Bernard CLESSE

Bonsoir à toutes et tous,Pourriez-vous m'aider à

14-04-2024 22:58

Bactridium flavum
B Shelbourne B Shelbourne

• Bactridium flavum (anamorph): Distinctive macr

15-04-2024 14:37

Cyphelle sp ?
Eric Rousseau

Bonjour,Je sais que les cyphelles ne sont pas des

13-04-2024 21:10

Cistella on dicot
B Shelbourne B Shelbourne

• Genus Cistella on dicots: Habitat, macro, hair

08-04-2024 19:57

Espèce inconnue
Elisabeth Stöckli

Bonsoir,Récolté au bord du chemin, apothécie 0.

15-04-2024 16:09

Pseudombrophila
Sylvie BIANCARDINI

Bonjour,Trouvé cet ascomycète sur vieille bouse

07-04-2024 20:49

Calloria urticae
B Shelbourne B Shelbourne

Another species that appears easy to identify from

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Chris
Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.