Accès membres

Mot de passe perdu? S'inscrire

12-11-2025 21:47

sp en restos de poda
ruiz Jose

Hola a todos, me envían esta colección en resto

12-11-2025 09:25

Pseudombrophila on soil
Viktorie Halasu Viktorie Halasu

Hello, I need help with a pale terrestric Pseudom

11-11-2025 20:16

yellow discs on a Quercus robur leaf
Bohan Jia

Hi, lastly I have found these tiny yellow decayin

09-11-2025 13:20

Ascomycete on Rubus.
Josep Torres Josep Torres

Hello.A tiny ascomycete, appearing as erupting gra

08-11-2025 12:10

Phacidium
Elisabeth Stöckli

Bonjour, Trouvé sur tiges mortes de Rubus (ronce

08-11-2025 00:29

Allophylaria subliciformis?
Francois Guay Francois Guay

I found this species in Quebec, Canada, on herbace

04-11-2025 14:53

Leptosphaeria?
Josep Torres Josep Torres

Hello.Very small, globose, mucronate perithecia, b

08-11-2025 09:15

Mollisia sur Pinus
Michel Hairaud Michel Hairaud

Bonjour, Pouvez vous m'aider à identifier ce Mol

06-11-2025 16:50

Hymenoscyphus sp?
Rot Bojan

Hello! Yesterday I found a fungus on or near a nee

05-11-2025 11:33

Cladosporium ?
Pierre Repellin

Bonjpur,J'ai trouvé, sur une hampe florale d'Alli

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Chris
Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.