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Khomenko Igor Khomenko Igor

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Lachnellula with long spores - L. pseudofarinacea L. ellisiana?
Ethan Crenson, 14-06-2021 01:16
Hello all,
About a week and a half ago in a cemetery in Brooklyn NY I found what I believe is a Lachnellula on the bark of Pinus flexilis. The apothecia are tiny, with a yellow hymenium clothed in white hairs.

The hairs are septate, tapering, smooth or minutely punctate. Paraphyses extend well beyond the asci and taper to an almost acute point. They appear to contain oil droplets.


Asci are 61-72 x 6-7µm, IKI+, croziers+ (please correct me if I am wrong).


Spores are long with acute ends, 15-43 x 2-4µm. The spores might range somewhat longer than my measurements—in looking back over my photos I see what may be broken spores that I ignored because I thought they were broken paraphyses. (see the last photo)


I will probably have to return to the place of collection and look again to get more details about the hairs and the true spore measurements. But maybe someone can point me in the right direction with the limited information I have presented here?


Thanks,
Ethan

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Juuso Äikäs, 14-06-2021 04:44
Re : Lachnellula with long spores - L. pseudofarinacea L. ellisiana?
I'm pretty sure this is not L. pseudofarinacea. That one has clearly longer and narrower spores and a hemiamyloid IKI reaction (red prior to KOH treatment).

Using Zotto's key


https://in-vivo-veritas.de/wp-content/uploads/2015/10/Lachnellula-Baral-20081.pdf


L. ellisiana seems to be the closest match.

Ethan Crenson, 14-06-2021 04:57
Re : Lachnellula with long spores - L. pseudofarinacea L. ellisiana?
Thank you Juuso.  I am very glad you pointed out that key as well!
Hans-Otto Baral, 14-06-2021 07:31
Hans-Otto Baral
Re : Lachnellula with long spores - L. pseudofarinacea L. ellisiana?
This is superb, yes, it is obviously L. ellisiana! It's the first time I see photos of this species. Mystudies relied on the type and a sample from 1978.

Croziers are clearly present, with a small perforation as typical in Lachnellula.

It would be great to gather more apothecia. Please take care also of living asci which should be greater than those you indicated which are narrower than the dead ones I measured.

Did you use a constant zoom in your photos? Coolpix under maximum zoom? It would be valuable to have a scale for them.

Also I would be glad to note the colletcion data as I have a long-lasting paper in preparation about this and some other Lachnellulas.

Not to forget that to say this species is so far unsequenced.

Zotto
Edouard Evangelisti, 14-06-2021 10:29
Edouard Evangelisti
Re : Lachnellula with long spores - L. pseudofarinacea L. ellisiana?
Hello,
I am happy to offer ITS sequencing for this species. If you are interested, please drop me a message.

Cheers,
Edouard
Ethan Crenson, 14-06-2021 16:52
Re : Lachnellula with long spores - L. pseudofarinacea L. ellisiana?
This is quite exciting.  Certainly a new species for New York City.  I will try to gather another sample.  My photos were taken with an iPhone 5.  Unfortunately I don't currently have a means of including the measurements in my microphotos.  The collection is from 2 June 2021, Lat: 40.652729 Lon: -73.992377 on bark of Pinus flexilis.  I may have an opportunity to sequence this if all of the stars align.