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03-07-2025 20:08

Francois Guay Francois Guay

I found this interesting yellowish asco growing on

28-06-2025 16:00

Josep Torres Josep Torres

Hello.A tiny fungus shaped like globose black grai

20-06-2025 08:33

Josep Torres Josep Torres

Hello.Small, blackish, mucronated surface grains s

01-07-2025 23:37

Josep Torres Josep Torres

Hello.A Pleosporal symbiotic organism located and

02-07-2025 17:26

Yanick BOULANGER

BonjourRécolté sur une brindille au fond d'un fo

03-07-2025 18:40

Castillo Joseba Castillo Joseba

me mandas el material seco de Galicia (España) re

02-07-2025 18:45

Elisabeth Stöckli

Bonsoir,Sur feuilles d'Osmunda regalis (Saulaie),

02-07-2025 09:32

Nicolas VAN VOOREN Nicolas VAN VOOREN

Hello, bonjour.Here is the paper I'm searching for

30-06-2025 16:56

Lydia Koelmans

Please can anyone tell me the species name of the

30-06-2025 12:09

Edvin Johannesen Edvin Johannesen

This tiny, rather "rough" erumpent asco was found

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Ayuda con un Helotial
Josep Torres, 12-09-2019 16:57
Josep TorresHola.
Un Helotial del pasado 8 de Septiembre sobre una piña muy degradada de Pinus sylvestris, semisumergida en un arroyo de agua limpia.
Apotecios de color blanco de entre 1,3 a 1,5 mm. en forma de disco con un corto y rudimentario pié.
Hifas del excípulo ectal de textura angularis de entre 8,3 a 16,4 µm.
Hifas del excípulo medular de estructura intricada, con tabiques simples y con una anchura de entre 3,2 a 4,4 µm.
Contorno con unas hifas terminales a modo de rudimentarios pelos con unas medidas de entre 29 a 37 µm. de largo por 3,2 a 3,9 µm. de ancho.
Ascas octospóricas de entre 66 a 77 µm. por 8,2 a 10,6 µm., con reacción amiloide del aparato apical al IKI (+) y al parecer sin uncínulos (croziers -) en su base.
Paráfisis con contenido VO, y con una anchura de entre 3,2 a 4,7 µm.
No se observa reacción amiloide en la base (-)
Ascosporas entre elipsoides y fusiformes, con un extremo mas apuntado y pequeñas gotas en su interior, con unas medidas de:
(9.2) 10 - 12.5 (13) × (4.7) 4.9 - 5.3 (5.7) µm
Q = (1.8) 2.1 - 2.5 (2.6) ; N = 30
Me = 11.2 × 5.1 µm ; Qe = 2.2
Intuyo que podría tratarse quizás de Phaeohelotoium, aunque no termina de encajarme con ninguno, sobre todo por el hecho de aparecer sobre una `piña de conífera y la no reacción de la base del mismo al yodo.
Cualquier sugerencia será bien recibida.
Muchas gracias de antemano.
Saludos cordiales.
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Mirek Gryc, 12-09-2019 17:05
Re : Ayuda con un Helotial
Hymenoscyphus kathiae?
Hans-Otto Baral, 12-09-2019 17:19
Hans-Otto Baral
Re : Ayuda con un Helotial
Yes, but the low oil content in the spores points to the very similar H. imberbis.
Zotto
Josep Torres, 12-09-2019 17:31
Josep Torres
Re : Ayuda con un Helotial
Muchas gracias a los dos.
Ya había pensado en Hymenoscyphus imberbis = Phaeohelotium imberbe, aunque en este caso ¿No debería reacciónar la base al IKI?. y además sobre cono de conífera.
de nuevo MUCHAS GRACIAS
Saludos cordiales.
Hans-Otto Baral, 12-09-2019 17:35
Hans-Otto Baral
Re : Ayuda con un Helotial
Substrate is rather unimportant and the blue reaction at the apo base not constistent as far as I remember. Another idea I do not have. Spore size seems to fit, though at the upper range in width.
Josep Torres, 12-09-2019 17:58
Josep Torres
Re : Ayuda con un Helotial
Gracias Zotto, queda pues como posible Hymenoscyphus imberbis
Saludos cordiales
Mirek Gryc, 12-09-2019 20:38
Re : Ayuda con un Helotial
Hi Zotto! Hi Josep!
I wasn't sure?
Oil drops in the spores indicate Hymenoscyphus imberbis.
Spore dimensions are more suitable for H. katiae?

I forgot about my 2018 collection.
Spores slightly smaller but I described it as H. imberbis :)
best regards
Mirek

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Hans-Otto Baral, 12-09-2019 20:52
Hans-Otto Baral
Re : Ayuda con un Helotial
Ah, on the same substrate! Not sure how much H. imberbis varies in spore size, but I would say that H. kathiae has just the same spore size as H. imberbis.

Mirek Gryc, 12-09-2019 21:03
Re : Ayuda con un Helotial
In the collections I found so far in almost all collections, the spore length was:
H. katiae> 10 um
H. imberbis <10 um
Mirek Gryc, 12-09-2019 22:06
Re : Ayuda con un Helotial
Hymenoscyphus kathiae.
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Mirek Gryc, 12-09-2019 22:09
Re : Ayuda con un Helotial
Hymenoscyphus imberbis
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Hans-Otto Baral, 12-09-2019 22:23
Hans-Otto Baral
Re : Ayuda con un Helotial
H. imberbis is genetically not easy as I remember, and it may be that we are talking of different taxa. My first samples of H. imberbis had spores *11-12.5 x 4.5-5.5 or *9-12 x 3.5-4 (HB 1003, 1007), and various later ones concurred with this. But I see also a short-spored one on Reynoutria (HB 6308). In the dead state the spores are smaller, 8-9.5 µm.
Mirek Gryc, 13-09-2019 10:50
Re : Ayuda con un Helotial
Oh yes! I meet a lot of collections whose features do not quite correspond to this species.
That's why I put photos of spores only those collections whose other features are 100% compatible with this species.

I used to send you pictures of fruiting bodies in which spores were about 1 um narrower. Other features were consistent with this species.


For me, H. imberbis, H. katiae and H. vernus are common species, but from the collections collected about 70-80% correspond exactly to these species. In others, there are features that are not typical of them?


Spain has a completely different atmosphere, so there may be slight differences in construction? :)


I only study fresh fruiting bodies!


That's right! - your collections have longer spores but other features are compatible with my :)
I have stopped microscoping them but the topic seems interesting enough to download a few more collections and compare their features more accurately!
best regards
Mirek

Hans-Otto Baral, 13-09-2019 10:57
Hans-Otto Baral
Re : Ayuda con un Helotial
Different climates may indeed play a role, but my data come mainly from Germany, and Poland does  not much differ in the plant communities. This group needs DNA work, also because anguillospora-like anamorphs are connected to the teleomorphs and will have priority in case no older teleomorph epithet exists.