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14-08-2016 23:15

Alex Akulov Alex Akulov

Dear friendsCan you help me to find the descriptio

04-06-2026 23:53

Stip Helleman Stip Helleman

Dear all, someone can get acces to this article f

05-06-2026 11:02

Thomas Læssøe

https://svampe.databasen.org/observations/10596691

04-06-2026 11:36

Gernot Friebes

Hi,found on Vaccinium myrtillus.Asci: IKI –, 8-s

05-06-2026 12:10

François Freléchoux François Freléchoux

Capitotricha sp. sur Lonicea caerulea Caractères

14-12-2014 18:31

Salvador Tello

Hola a todos. Tengo estos hongos que cogí ayer q

19-05-2026 10:27

Patrice TANCHAUD

Bonjour, récolte récente sur terre retournée i

04-06-2026 18:39

Gernot Friebes

Hi,I collected this species in two different locat

22-05-2026 13:29

Gernot Friebes

Hi,I am curious to hear your opinion on this mater

04-06-2026 15:28

Tony Cumberlidge

I have recently found the very helpful European Ke

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul