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Mot de passe perdu? S'inscrire

22-08-2012 23:01

Bometon Javier Bometon Javier

Fructificaciones de aproximadamente 10mm,  un poc

22-08-2012 15:52

Alain GARDIENNET Alain GARDIENNET

Bonjour, Dans des tiges de Typha angustifolia, au

22-08-2012 21:00

Marja Pennanen

Hi,I did not manage to determine these hairy cups

20-08-2012 21:57

Michel Hairaud Michel Hairaud

Bonsoir . Hi to all. Pourriez vous me confirmer q

21-08-2012 09:53

Alessio Pierotti Alessio Pierotti

The genus Polystigma was erected by Persoon in 181

16-08-2012 12:27

Stefan Blaser

Hi allI'm looking for Literature to identify Cosmo

15-08-2012 11:56

Piet BORMANS Piet BORMANS

It looks a little bit like Acanthophiobolus helico

17-08-2012 12:40

Alessio Pierotti Alessio Pierotti

Mi sono imbattuto nell'ordine Phyllachorales...dov

16-08-2012 09:21

John Plischke John Plischke

Found growing in Pennsylvana, United States.  Gro

15-08-2012 13:46

Enrique Rubio Enrique Rubio

Hi to everybody:This Scutellinia grews on sandy so

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul