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05-08-2011 16:35

Hans-Otto Baral Hans-Otto Baral

Hiwe have here something we think is a Trichonectr

04-08-2011 04:52

Roland Labbé

Bonjour !Voici une planche d'un Disco carbonicole

04-08-2011 15:12

Marja Pennanen

Hello forum,when studying Perichaena I noticed som

03-08-2011 23:36

Alain GARDIENNET Alain GARDIENNET

Bonjour, Quelqu'un aurait-il une clé du genre Xen

02-08-2011 18:24

Beñat Jeannerot Beñat Jeannerot

Adio, Une Peziza. Pédehourat, 64, 01 Août 2011

03-08-2011 12:49

Yatsiuk Iryna Yatsiuk Iryna

Dear friends! I have 2 Peziza specimens for your

02-08-2011 20:24

Martin Bemmann Martin Bemmann

Dear forists,I still seek this paper:Svrcek, M., C

02-08-2011 18:16

Beñat Jeannerot Beñat Jeannerot

Adio, Une Peziza. Je n'y connais rien, donc je so

01-08-2011 16:22

Casares Pablo

Hello. I'm reading this forum for the first time a

31-07-2011 10:18

Michel Hairaud Michel Hairaud

Hi to everyone , Bonjour à tous, Trochila lauroce

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul