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23-12-2025 08:27

Josep Torres Josep Torres

Hello.Small, yellowish ascomata, with very short a

07-03-2026 22:22

Josep Torres Josep Torres

Hello.Some very tiny conidiomes collected in the D

07-03-2026 13:06

éric ROMERO éric ROMERO

Bonjour tous, Sur cône d'épicea fortement imbu,

06-03-2026 17:51

William Slosse William Slosse

Good evening all,On March 4, 2026, I found the fol

05-03-2026 16:30

François Bartholomeeusen

Dear forum members, On the 2nd of February 2026,

06-03-2026 09:41

Alain GARDIENNET Alain GARDIENNET

Hi forum, I'm now looking for another reference c

28-02-2026 11:05

Yanick BOULANGER

Bonjour à tousLe 24/02/2026 à Montmacq, devant m

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Hulda Caroline Holte

Hello, I found and collected this species growing

05-03-2026 19:29

William Slosse William Slosse

Good evening everyone,On March 4, 2026, I found th

19-02-2026 17:49

Salvador Emilio Jose

Hola buenas tardes!! Necesito ayuda para la ident

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul