Accès membres

Mot de passe perdu? S'inscrire

24-04-2024 21:54

éric ROMERO éric ROMERO

Bonjour, J'ai trouvé ce Lasiobolus sur laissées

23-04-2024 15:18

Lothar Krieglsteiner Lothar Krieglsteiner

... but likely a basidiomycete. I hope it is o.k.

23-04-2024 13:17

Edouard Evangelisti Edouard Evangelisti

Bonjour à tous, Je viens de récolter ce que je

23-04-2024 21:49

Ethan Crenson

Hello all, A friend recently found this orange as

22-04-2024 11:52

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello,I made a loan of a collection of Microstoma

11-01-2022 16:36

Jason Karakehian Jason Karakehian

Hi does anyone have a digital copy of Raitviir A (

22-04-2024 08:54

Rafael Cabral

Bonjour à toutes et tous, Quelqu'un pourrait-il

22-04-2024 20:38

Miguel Ãngel Ribes Miguel Ángel Ribes

Good afternoon.Does anyone know this anamorph?It g

21-04-2024 14:29

B Shelbourne B Shelbourne

• Genus Brunnipila: Distinct macro and habitat,

19-04-2024 14:28

B Shelbourne B Shelbourne

Cudoniella tenuispora: Distinctive macro and habit

« < 1 2 3 4 5 > »
Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul